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ALY688 (100 and 300 nM) induced AMPK phosphorylation in epididymal (Epid, A) and subcutaneous inguinal (Sc Ing, B) adipocytes. AdipoRon (AR) was used as a positive control. In Sc Ing adipocytes, a higher dose of ALY688 also increased the phosphorylation of AMPK (c) and ACC (d). Different letters denote statistical significance (p < 0.05). One-way ANOVA for A-B and t-test for C-D, n = 3
