Figure 7. Effect of preventive and therapeutic HIF-1α and HIF-2α ASO treatment on hepatic protein expression of inflammatory markers in DEN-induced HCC mice.

HCC was induced by weekly intraperitoneal DEN injection for 25 weeks. Control mice received weekly intraperitoneal 0.9% NaCl injection. DEN-treated mice were intraperitoneally injected with 20 mg/kg HIF-1α ASO, HIF-2α ASO or scrambled ASO twice per week, in either a preventive or a therapeutic setting. Control mice received scrambled ASO for the same duration of the experiment. Hepatic protein expression of several inflammatory markers following preventive and therapeutic treatment is shown. The upper and lower dashed lines represent mean ± SD of the control mice. Bars represent mean ± SD of different treatment groups of DEN-treated mice (n = 7–9 per treatment group). ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001 and ^**** p < 0.0001.