Figure 3.

ABA induces G₀ cell cycle arrest in PCa cells. (A) A flow profile of the cell cycle phase; G_0, G₁, and S/G₂/M in PC3^VC cells. (B) % of G_0, G_1, or S/G₂/M cell cycle phase and (C) Ki67 expression in PC3^VC cells following the treatment of vehicle or ABA (50 µM) in 1% FBS or 10% FBS culture conditions at 72 h as quantified by FACS analyses. Data in B and C are representative of mean with s.d. (Student's t test). (D) Expression of p27, p21, and p16 by vehicle or ABA treatment as quantified by Western blot. (E) Live cell imaging of PC3^VC (G₀: yellowish-orange arrow, G₁: red arrow, and S/G₂/M: white arrow) following vehicle or ABA (50 µM) treatment by video for 24 h with 15 min interval by Deltavision Elite Microscope. Bar=25 µm. Insert: The color scheme indicates the cell cycle phase, either quiescenct /G₀ (orange)_,G₁ (red) and the combination of S, G2 and M phases (none). (F) Quantification of G₀ duration in single cells from E (n = 10/group). Data are representative of mean with SD (Student's t test).