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. 2020 Dec 7;6(12):e05574. doi: 10.1016/j.heliyon.2020.e05574

Figure 4.

Figure 4

Monitoring of ERK activity pulses upon low doses of EGF. a. Median Cyto/Nucl ratio of ERK-SKARS in more than 400 single cells from a clonal HeLa cell population stimulated with low doses of EGF and monitored for more than 3 h b. Fraction of cells identified in the experiment from panel 4a that display three pulses or more in ERK activity. c. Heat map of the Cyto/Nucl ratio measured upon 0.1 ng/ml EGF treatment in HeLa cells. Only cells where three or more pulses were identified are displayed in this graph. d. Microscopy images of HeLa cell displaying pulses in ERK activation upon 0.1 ng/ml EGF stimulation. e. Various examples of single cell traces displaying fluctuations in ERK activation upon 0.1 ng/ml EGF treatment.