Corrigendum: Dynamic nature of SecA and Its associated proteins in Escherichia coli

In the original article, there was a mistake in Figure 2 as published. Panel J in the original figure was incorrectly labeled “SecA-GFP secA204.” Panel J in the corrected figure appearing below is now labeled “SecY-GFP secA204” in agreement with panels F to I presented in this section of the figure.

Figure 2.

Time-lapse images of GFP_uv4-fused proteins reveal the dynamic nature of chromosome partitioning proteins. Cells were grown in medium C and incubated with 0.1 mM IPTG for 1 h. Time-lapse images of fluorescence were taken by exposure for 100 ms. Both images and histograms of signals along long cell axes were obtained at 45-s intervals. The x-axes of histograms are subcellular positions and the y-axes of histograms are relative fluorescence units. Ninety-five Percent confidential SD (standard deviation) values of fluorescence signals along long cell axes in individual cells are also shown. (A) SecA-GFP_uv4 in wild-type cells (MQ318) growing at 30°C. (B) SecA-GFP_uv4 in secY39(Cs) mutant cells (MQ435) growing at the permissive temperature of 37°C. (C) SecA-GFP_uv4 in secY39(Cs) mutant cells (MQ435) growing at the non-permissive temperature of 22°C. (D) SecA-GFP_uv4 in acpPD38V(Ts) mutant cells (MQ456) growing at the permissive temperature of 30°C. (E) SecA-GFP_uv4 in acpPD38V(Ts) mutant cells (MQ456) growing at the non-permissive temperature of 42°C. (F) SecY-GFP_uv4 in wild-type cells (MQ319) growing at 30°C. (G) SecY-GFP_uv4 in secA51(Ts) mutant cells (MQ748) growing at the permissive temperature of 30°C. (H) SecY-GFP_uv4 in secA51(Ts) mutant cells (MQ748) growing at the non-permissive temperature of 42°C. (I) SecY-GFP_uv4 in wild-type cells (MQ319) with 1 mM sodium azide growing at 30°C. The incubation time with azide was approximately 5 min. (J) SecY-GFP_uv4 in secA204 azide-resistant mutant cells (MQ625) growing at 30°C (without sodium azide). (K) ParC-GFP_uv4 in wild-type cells (MQ537) growing at 30°C. (L) ParE-GFP_uv4 in wild-type cells (MQ539) growing at 30°C. (M) GyrA-GFP_uv4 in wild-type cells (MQ323) growing at 30°C. (N) GyrB-GFP_uv4 in wild-type cells (MQ324) growing at 30°C. (O) MukB-GFP_uv4 in wild-type cells (MQ529) growing at 30°C. (P) GFP_uv4 in wild-type cells (MQ668) growing at 30°C.

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.