Figure 4.

Alignment of RdRp sequences from members of the (a) Betacoronavirus and (b) Flavivirus genus. The sequences alignment was carried out using the ClustalX version 2.1 [54] with the pairwise alignment algorithm, keeping the default color scheme for the amino acids, i.e., blue = hydrophobic; red = positive charge; magenta = negative charge; green = polar; pink = cysteine; orange = glycine; yellow = proline; cyan = aromatic; white = unconserved or gaps. The black bar below the alignment corresponds to the level of conservation: the higher the bar, the higher the conservation at every position. * indicates the amino acids that are conserved in the aligned sequences.