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. 2020 Nov 24;9:e54501. doi: 10.7554/eLife.54501

Figure 2. Sexually dimorphic expression of ORs is consistent with a change in the number of OSNs expressing those ORs.

(A) Left: representative in situ mRNA hybridization pictures probing for the expression of Olfr910 in 43-week-old sex-separated male (top) and female (bottom) mice. Scale bars indicate 50 μm. Right: summary data showing mean and SEM of the density of OSNs expressing Olfr910 in 43-week-old male and female mice. An unpaired two-tailed t-test revealed statistical difference (****p < 0.0001) between males and females. Data are from n = 3 male and n = 3 female mice. (B) Representative in situ mRNA hybridization pictures probing for the expression of Olfr912 in 43-week-old sex-separated male (top) and female (bottom) mice. Right: summary data showing mean and SEM of the density of OSNs expressing Olfr912 in 43-week-old male and female mice. An unpaired two-tailed t-test revealed statistical difference (****p < 0 .0001) between males and females. (C) Left: representative in situ mRNA hybridization pictures probing for the expression of Olfr1295 in 43-week-old sex-separated male (top) and female (bottom) mice. Right: summary data showing mean and SEM of the density of OSNs expressing Olfr1295 in 43-week-old male and female mice. An unpaired two-tailed t-test revealed statistical difference(****p < 0 .0001) between males and females.

Figure 2—source data 1. Summary statistics for Figure 2.

Figure 2.

Figure 2—figure supplement 1. Architecture of reads uniquely mapping to female-enriched ORs.

Figure 2—figure supplement 1.

(A) Architecture of reads uniquely mapped to Olfr910 in male and female mice. Shown are the defined transcript variants, coding sequence (CDS), uniquely designed riboprobe, and 5′ to 3′ directionality (red arrows). The y-axis shows the number of mapped reads along genomic coordinates described on the x-axis. Reads crossing exon junctions are also quantified. (B) Architecture of reads uniquely mapped to Olfr912. (C) Architecture of reads uniquely mapped to Olfr1295.