Figure 5. OSN responses to semiochemicals are not sexually dimorphic between mature male and female mice.

(A) Comparison of responses of OSNs from 26-week-old male and female mice to various stimuli. One-way ANOVA with Tukey’s multiple comparisons test correction reveals only exposure to 0.1% (v/v) SBT leads to significant pS6 induction in OSNs expressing Olfr910 (***p < 0.001) with no significant differences between males and females (ns; p > 0.05). Data are from n = 3 male and n = 3 female mice. (B) One-way ANOVA with Tukey’s multiple comparisons test correction reveals only exposure to 0.1% (v/v) SBT leads to significant pS6 induction in OSNs expressing Olfr912 (**p < 0.01, ****p < 0.0001) with no significant differences between males and females (ns; p > 0.05). (C) One-way ANOVA with Tukey’s multiple comparisons test correction reveals only exposure to 10 mM MTMT leads to significant pS6 induction in OSNs expressing Olfr1295 (****p < 0.0001) with no significant differences between males and females (ns; p > 0.05).

Figure 5—figure supplement 1. Example in situ stainings showing responses to semiochemicals are not sexually dimorphic between mature male and female mice.

(A) Representative images from mature male and female mice showing in situ mRNA hybridizations probing for Olfr910 expression and pS6 immunostainings. Co-localization events are only seen following SBT exposure as indicated by arrowheads. Scale bars indicate 20 μm. (B) Representative images from mature male and female mice showing in situ mRNA hybridizations probing for Olfr912 expression and pS6 immunostainings. Co-localization events are only seen following SBT exposure. (C) Representative images from mature male and female mice showing in situ mRNA hybridizations probing for Olfr1295 expression and pS6 immunostainings. Co-localization events are only seen following MTMT exposure.