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. 2021 Jan 1;11(2):861–877. doi: 10.7150/thno.48436

Figure 3.

Figure 3

PBA activates the Nrf2 signaling pathway. (A) PBA decreased ROS production. Cells were treated with the indicated concentrations of PBA, and then stimulated with LPS plus ATP, and the ROS level was determined by the fluorescent probe DCFH-DA. The results are expressed as the mean ± SD (n = 3). (B) PBA promoted Nrf2 translocation into the nucleus. The cells were treated with PBA for 6 h. After isolation of the proteins in the cytosol and nucleus, the protein extract was subjected to SDS-PAGE for western blotting analysis. Other cells were used for Nrf2 immunofluorescence staining. Scale bar: 10 μm. (C) PBA increased Nrf2 expression and HO-1 expression in the OGD/R-induced cardiomyocytes. *p < 0.05, **p < 0.01, ***p < 0.001, OGD/R + PBA vs OGD/R. (D) The effects of PBA on HO-1 and NQO1 expression. Cells were treated with PBA for 24 h. *p < 0.05, **p < 0.01, PBA treatment vs the control.