Figure 7.

Effect of Ca²⁺-dependent signaling on AA-mediated phosphorylation of p38. (A) Increase in intracellular Ca²⁺ levels 0-105 sec after stimulation with 50 µM AA or ETYA. Kinetic measurements of Fura-2 fluorescence were carried out as described in Materials and Methods. (B) AA-mediated phosphorylation of p38 and CAMK IIγ and δ, and its inhibition by the Ca²⁺ chelator BAPTA-AM. The cells were pretreated with 50 µM BAPTA-AM for 1 h prior to treatment with 50 µM AA for 30 min. (C, D). Quantification of 8 biological replicates as in panels A and B. Data points represents individual samples, horizontal lines indicate the median. Statistical analysis was performed by paired t test: **** adjusted p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05 against AA-treated samples.