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. 2020 Dec 16;6(51):eabe6393. doi: 10.1126/sciadv.abe6393

Lamellar cells in Pacinian and Meissner corpuscles are touch sensors

Yury A Nikolaev 1, Viktor V Feketa 1,2,3, Evan O Anderson 1,*, Eve R Schneider 1,, Elena O Gracheva 1,2,3,, Sviatoslav N Bagriantsev 1,
PMCID: PMC7744075  PMID: 33328243

Tactile corpuscles in vertebrate skin use both neuronal and non-neuronal mechanoreception to detect mechanical signals.

Abstract

The skin covering the human palm and other specialized tactile organs contains a high density of mechanosensory corpuscles tuned to detect transient pressure and vibration. These corpuscles comprise a sensory afferent neuron surrounded by lamellar cells. The neuronal afferent is thought to be the mechanical sensor, whereas the function of lamellar cells is unknown. We show that lamellar cells within Meissner and Pacinian corpuscles detect tactile stimuli. We develop a preparation of bill skin from tactile-specialist ducks that permits electrophysiological recordings from lamellar cells and demonstrate that they contain mechanically gated ion channels. We show that lamellar cells from Meissner corpuscles generate mechanically evoked action potentials using R-type voltage-gated calcium channels. These findings provide the first evidence for R-type channel-dependent action potentials in non-neuronal cells and demonstrate that lamellar cells actively detect touch. We propose that Meissner and Pacinian corpuscles use neuronal and non-neuronal mechanoreception to detect mechanical signals.

INTRODUCTION

The sense of touch is essential for a range of physiological processes, including detection of pain and pleasure, object recognition, foraging, and environment navigation. It facilitates the establishment of maternal bonds and underlies the development of social behaviors (1). The human palm contains a dense population of mechanosensory end-organs, including Pacinian, Meissner, and Ruffini corpuscles, along with Merkel cell-neurite complexes. The same or analogous structures are present in the skin of most vertebrates (2). Mechanosensory end-organs are thus essential for precise manipulation of tools and objects, and performing fine tactile tasks (35). Animals that are mechanosensory specialists have organs that are functionally analogous to the human palm, including the star organ of the star-nosed mole and the bill of tactile-foraging waterfowl. These organs contain hundreds of mechanosensory corpuscles per square millimeter of skin, allowing mechanosensory specialists to rely on touch during their search for food (2, 68).

The two most numerous types of mechanosensory corpuscles in the human palm are Pacinian and Meissner corpuscles. Pacinian corpuscles detect high-frequency vibration, whereas Meissner are tuned to lower frequencies (2, 5, 9). Both types are innervated by myelinated mechanoreceptors that arise from somatosensory ganglia. Neuronal mechanoreceptors are thought to be the only touch sensors within corpuscles and produce rapidly adapting firing patterns when their mechanically gated ion channels are activated by touch (4, 10, 11). In Pacinian corpuscles, the mechanoreceptor is surrounded by onion-like sheaths formed by lamellar cells, whereas it is sandwiched between two or more lamellar cells in Meissner corpuscles. The functional role of lamellar cells is obscure, but they are thought to provide structural support for the neuronal afferent, facilitate small-amplitude vibrations (12), and serve as a passive mechanical filter for static stimuli (13). There are reports that some lamellar cells are immunoreactive for synaptic proteins, suggesting an active, rather than passive, role in touch sensing (1416). However, despite their widespread presence in vertebrates, no functional studies of lamellar cells from Meissner- or Pacinian-like corpuscles from any species exist, and their physiological roles remain unknown (15).

RESULTS

To test whether lamellar cells play an active role in the detection of touch, we developed a glabrous skin preparation from the bill of Pekin duck, a tactile-specialist bird (2, 17). Duck bill skin contains a dense population of Pacinian- and Meissner-like corpuscles, referred to as Herbst and Grandry corpuscles, respectively (18, 19). Like their mammalian counterparts, duck corpuscles are innervated by rapidly adapting myelinated mechanoreceptors and are tuned to detect transient pressure and vibration (1922). Optical and electron microscopic analyses of an ex vivo preparation of duck bill skin (Fig. 1A and Materials and Methods) revealed a mixed population of Pacinian and Meissner corpuscles, which could be distinguished by their unique morphology and size (Fig. 1, B and C). Duck Pacinian corpuscles had an oval structure, ~35 to 120 μm in size (n = 140 corpuscles), and comprised a mechanoreceptive neuronal afferent surrounded by an inner core and outer capsule formed by lamellar cells (Fig. 1, D to F). Duck Meissner corpuscles were more spherical and smaller in size (~15 to 35 μm in diameter, n = 50 corpuscles) than Pacinian corpuscles and consisted of a neuronal mechanoreceptor surrounded by two or more lamellar cells (Fig. 1, G to I) (14, 18). Although the number of outer core layers in duck Pacinian corpuscles and the number of lamellar cells in duck Meissner corpuscles are lower than the number of analogous structures in humans, the overall architecture of duck corpuscles is similar to their human counterparts. In addition, our electron microscopy images showed that lamellar cells from duck Meissner corpuscles contain dense core vesicles (fig. S1), similar to the structures reported in Meissner lamellar cells from humans (14, 23). The presence of Pacinian and Meissner corpuscles in duck bill skin suggests that it as a good model system for the human palm. In contrast, mouse glabrous skin normally lacks Pacinian corpuscles, which are located in the periosteal membrane (24).

Fig. 1. The bill skin of a tactile-specialist duck has Pacinian and Meissner corpuscles.

Fig. 1

(A) Schematic illustration of the preparation of duck bill skin for electrophysiological and optical analysis of mechanosensory corpuscles. (B) A bright-field microscopic image of a mixed population of Pacinian corpuscles (blue arrowheads) and Meissner corpuscles (red arrowheads) in a patch of duck skin from the dorsal surface of the upper bill. (C) Size distribution of visible Meissner and Pacinian corpuscles in duck bill skin (50 Meissner and 140 Pacinian corpuscles in total). (D to I) Illustrations (D and G), electron microscopy images (E and H), and close-up bright-field microscopy images (F and I) of mechanosensory corpuscles. Pacinian corpuscles are composed of outer core lamellar cells surrounding an inner bulb of inner core cells and a neuronal mechanoreceptor. In Meissner corpuscles, the mechanoreceptor is sandwiched between two or more lamellar cells.

Having identified lamellar cells in mechanosensory corpuscles from duck bill skin, we sought to characterize them in situ by injecting the fluorescent dye Lucifer yellow using a patch electrode (Fig. 2, A and B). The dye remained confined within the volume of each cell for 15 min after injection, suggesting that a diffusion barrier existed between lamellar cells in both corpuscular types. The long, flat outer lamellar cells in Pacinian corpuscles had an average length of 11.42 ± 0.72 μm (means ± SEM, n = 7 cells; Fig. 2A). The hemispherical lamellar cells in Meissner corpuscles had an average diameter of 17.56 ± 1.65 μm (n = 7 cells; Fig. 2B). Electrophysiological recordings revealed that Pacinian and Meissner lamellar cells had a whole-cell membrane capacitance of 9.6 ± 1.4 and 24.6 ± 4.6 pF, respectively (Fig. 2C). In addition, Pacinian lamellar cells had a resting membrane potential of −51.9 ± 2.0 mV and a high apparent input resistance of 5.8 ± 1.8 GΩ, whereas Meissner lamellar cells had a significantly more negative resting potential of −73.5 ± 2.4 mV and lower input resistance of 1.5 ± 0.4 GΩ (Fig. 2C).

Fig. 2. Lamellar cells of Pacinian and Meissner corpuscles are mechanosensitive.

Fig. 2

(A and B) Representative bright-field (left) and fluorescent images (right) of lamellar cells from Pacinian and Meissner corpuscles filled with Lucifer yellow via the recording electrode. A glass probe is positioned nearby to deliver mechanical stimulation. (C) Electrophysiological characteristics of lamellar cells. Significance was calculated using unpaired two-tailed t test. Open circles denote individual cells. (D) Representative MA currents elicited from lamellar cells by mechanical indentation using a glass probe. (E) Quantification of peak MA current amplitude in Pacinian (left, n = 19 cells) and Meissner (right, n = 7 cells) lamellar cells in response to indentation with a glass probe. Lines connect measurements from individual cells. (F) Quantification of MA current rise time (τrise) recorded in lamellar cells and in duck trigeminal mechanoreceptors with fast, intermediate, and slow MA current. The difference between means is significant; F4,61 = 3.49, P = 0.013, one-way analysis of variance (ANOVA) with Tukey’s post hoc multiple comparisons test. Open circles denote individual cells. (G) Quantification of MA current inactivation rate (τdecay) recorded in lamellar cell and duck trigeminal mechanoreceptors. τdecay values greater than 1000 ms are plotted as 1000. Bars represent median. Open circles denote individual cells. The difference between medians is significant; P < 0.0001, Kruskal-Wallis test. ***P < 0.0001, Dunn’s post hoc multiple comparisons test. (H) Representative MA currents elicited from lamellar cells in response to indentation at different voltages. (I) Voltage dependence of peak MA current from eight Pacinian and five Meissner lamellar cells, fitted to the linear equation. (J) Quantification of MA current τdecay from seven Pacinian and seven Meissner lamellar cells, fitted to the linear equation. r, Pearson’s correlation coefficient; P denotes the probability of the line slope being equal to 0. Data are presented as means ± SEM from at least three independent skin preparations.

We next asked whether lamellar cells are mechanosensitive in situ. Stimulation of either Pacinian or Meissner lamellar cells with a glass probe produced robust mechanically activated (MA) currents, which increased in amplitude as probe displacement increased (Fig. 2, D and E, and fig. S2A). Although MA currents from Pacinian lamellar cells had a significantly slower rise time than Meissner cell currents (τrise = 2.8 ± 0.3 and 1.4 ± 0.2 ms for Pacinian and Meissner cells, respectively, P = 0.005), both values were within the range of MA currents recorded from mechanosensitive neurons (Fig. 2F) (25, 26). Following activation, Pacinian lamellar MA currents decayed (τdecay = 48.7 ± 7.0 ms), reaching 20 to 68% of their peak amplitude by the end of the 150-ms stimulus (Fig. 2D and fig. S2, B and C). In some cells, up to 30% fraction of peak MA current persisted after retraction of the probe and, in each case, returned to baseline within 10 s (fig. S2, B and D). The inactivation rate of Pacinian lamellar MA current was in the range of slowly inactivating neuronal mechanoreceptors (Fig. 2G). In contrast, Meissner lamellar MA currents decayed significantly faster (τdecay = 11.8 ± 2.3 ms, P < 0.0001 versus Pacinian lamellar MA current), similar to mechanoreceptors with fast and intermediate inactivation kinetics (2731), and lacked a persistent, noninactivating component (Fig. 2, D and G). Both types of MA current had a linear voltage dependence and a near-zero reversal potential (Fig. 2, H and I), characteristic of a nonselective cation conductance. However, they differed in their voltage dependence of inactivation: depolarization slightly decreased τdecay in Pacinian lamellar cells (P = 0.111) and increased τdecay in Meissner cells (P = 0.019; Fig. 2J).

Together, these data reveal that lamellar cells of Pacinian and Meissner corpuscles are intrinsically mechanosensitive. The fast activation kinetics of lamellar MA currents, linear voltage dependence, and lack of ion selectivity are consistent with the ion channel–based mechanotransduction mechanism in somatosensory neurons (28, 3235). The significant differences in the rate and voltage dependence of MA current decay between Pacinian and Meissner lamellar cells from duck bill skin indicate that they each express different mechanically gated ion channels or the same channels with modified function.

Given the similarities between lamellar cells and neuronal mechanoreceptors, we wanted to find out whether lamellar cells are excitable. We first asked whether they have voltage-activated conductances by depolarizing and hyperpolarizing their membranes to different test potentials. Such voltage stimulation of Pacinian lamellar cells failed to reveal voltage-activated potassium, sodium, or calcium currents (fig. S3, A and B). Moreover, depolarizing current injection failed to evoke any action potentials and instead induced a linear depolarization of the membrane with a slope averaging 2.7 mV/pA, typical of nonexcitable cells (fig. S3C). In contrast, lamellar cells from Meissner corpuscles displayed robust voltage-gated potassium currents (Fig. 3A). When these currents were blocked by replacing K+ with Cs+ in the patch electrode, we identified voltage-gated inward currents that were largely blocked by Cd2+ or depletion of extracellular Ca2+, suggesting that they were mediated by voltage-gated calcium (Cav) channels (Fig. 3, B to F). Ratiometric live-cell calcium imaging of duck bill skin revealed that high extracellular potassium–induced depolarization evoked an increase in intracellular calcium in lamellar cells of Meissner, but not Pacinian, corpuscles (Fig. 3, G to I), corroborating our finding that Meissner lamellar cells express Cav channels.

Fig. 3. Lamellar cells from Meissner corpuscles express voltage-activated channels.

Fig. 3

(A) Current traces and current-voltage (IV) plots of voltage-activated K+ currents (means ± SEM, n = 12 Meissner and 5 Pacinian lamellar cells). (B to E) Current traces and IV plots of voltage-activated Ca2+ currents in the presence of a general Cav channel blocker 300 μM Cd2+ [(B) and (C), n = 5 cells] and upon depletion of extracellular Ca2+ to 20 μM, low Ca2+ [(D) and (E), n = 7 Meissner and n = 7 Pacinian lamellar cells]. Data are means ± SEM. (F) Conductance-voltage relationship of Cav current, fitted to the Boltzmann equation, with half-maximal activation voltage (V1/2) of −23.5 ± 0.4 mV (means ± SEM, n = 12 Meissner lamellar cells). (G) Representative partial fields of view of live-cell ratiometric Fura-2 AM calcium imaging of Meissner (white arrowheads) and Pacinian (black arrowheads) corpuscles in duck bill skin. Application of 135 mM extracellular potassium (high K+) elevates intracellular calcium in lamellar cells of Meissner but not in Pacinian corpuscles or in the neuronal ending within the corpuscles (H) Example traces from Meissner corpuscles in response to application of high K+. Colors of the traces correspond to the color scale bar in (G) based on peak response value. (I) Quantification of peak calcium signal in Pacinian and Meissner corpuscles and in skin areas of comparable sizes devoid of corpuscles (background) in response to high K+. Dots represent individual data points. All data are from at least three independent skin preparations.

Having established that Meissner lamellar cells express voltage-gated ion channels, we asked whether they could fire action potentials. Depolarizing current injection triggered repetitive action potential firing in Meissner lamellar cells with a rheobase averaging 16.07 ± 1.9 pA (Fig. 4A and fig. S4A). The voltage-current relationship was strongly rectifying, characteristic of excitable cells (fig. S4B). In agreement with our finding that Meissner lamellar cells express Cav channels, the depletion of extracellular Ca2+ or addition of Cd2+ dampened firing (Fig. 4, A, B, and E), whereas tetrodotoxin, a blocker of voltage-gated sodium channels (Nav), did not (Fig. 4, C and E). Transcriptomic analysis revealed that several types of Cav channel alpha subunits were expressed in duck bill skin (Fig. 4F). However, pharmacological blockade of L-, N-, T-, and P/Q-type Cav channels failed to affect firing (Fig. 4E and fig. S5, A to E, G, and H), suggesting that although these channels could be expressed in Meissner lamellar cells, their activity is not critical for action potential generation. In contrast, SNX-482, a specific blocker of R-type (Cav2.3) channels, completely abolished action potential firing (Fig. 4, D and E, and fig. S5F). Thus, action potential firing in Meissner lamellar cells must be predominantly mediated by R-type Cav channels.

Fig. 4. Lamellar cells from Meissner corpuscles are excitable mechanosensors.

Fig. 4

(A to D) Exemplar action potentials (APs; left and middle) and spike quantification (right) obtained by current injection into Meissner lamellar cells in the presence of low (20 μM) extracellular Ca2+ [(A), n = 6 cells], pan-Cav blocker Cd2+ [(B), n = 4 cells], Nav blocker tetrodotoxin (TTX; (C), n = 5 cells], R-type Cav2.3 blocker SNX-482 [(D), n = 11 cells]. Thin lines in quantification panels represent individual cells; thick lines connect means ± SEM. (E) Pharmacological profile of Meissner lamellar cell firing upon injection of a 100-pA current. Letters indicate Cav type. Open circles represent individual cells. The effect of treatment is significant. F11,53 = 75.57, P < 0.0001, one-way ANOVA; ***P < 0.0001 versus control, Dunnett’s test. (F) Quantification of Cav alpha subunit expression in duck bill skin (n = 3 animals). FPKM, number of mRNA fragments per kilobase of exon per million fragments mapped. (G) Mechanical stimulation evokes action potential firing in Meissner lamellar cells. Shown are exemplar action potential traces (left) and quantification of mechanically evoked firing (right) from three Meissner lamellar cells (thin lines). Thick line connects means ± SEM. (H) The number of action potentials is maximal when MA current is at its peak. Shown is a cumulative frequency distribution of the number of action potentials (circles) evoked by mechanical stimulation of four Meissner lamellar cells to 8-μm depth, fitted to the single exponential equation (line) and plotted against peak-normalized MA current profile.

Because the rheobase for Meissner lamellar cell firing was comparable to the amplitude of MA current produced by direct mechanical stimulation, we wondered whether mechanical stimulation alone could elicit firing. Indentation with a glass probe triggered repetitive firing in Meissner lamellar cells with a threshold of 4.6 ± 0.4 μm (n = 7 cells; Fig. 4G and fig. S4C); the number of action potentials increased in proportion to the degree of indentation (Fig. 4G). Notably, the number of action potentials during mechanical stimulation exponentially decayed with a time constant τdecay = 16.6 ms, similar to the average τdecay for MA current in these cells (11.8 ms), further supporting the causative relationship between these events (Fig. 4H). Together, these data demonstrate robust mechanically evoked excitability in Meissner lamellar cells.

DISCUSSION

Here, we used tactile specialist ducks to investigate functional properties of lamellar cells from Meissner and Pacinian corpuscles. The exceptionally high density of corpuscles in duck bill skin and the accessibility of these structures to electrophysiological analysis suggest that this nonstandard model organism could complement rodent models in uncovering basic principles of mechanotransduction in glabrous skin, from the perspective of a tactile specialist. We have shown that Meissner lamellar cells are non-neuronal mechanosensors that can generate Ca2+-dependent action potentials via R-type Cav channels. To our knowledge, this is the only non-neuronal cell type that uses R-type Cav channels for firing. We detected mechanosensitivity, but not excitability, in Pacinian outer core lamellar cells. Nevertheless, because these cells have high input resistance and depolarize at a fast rate of ~2.7 mV/pA, the robust MA current that they produce (~8.0 pA/μm) is sufficient to completely depolarize the membrane in response to a ~2.5-μm indentation without the need for amplification via voltage-activated machinery. The MA currents produced by Pacinian and Meissner lamellar cells are different from each other and from MA currents produced by Piezo2, a mechanically gated ion channel with a prominent role in somatosensory mechanotransduction in vertebrates (19, 3542). The decay kinetics of Pacinian MA current is slow (τdecay-80 mV ~ 48.7 ms) and is weakly affected by voltage. In contrast, the decay kinetics of Piezo2 is fast (τdecay-80 mV < 10 ms) and becomes significantly slower with depolarization (35, 43). The MA current produced by Meissner lamellar cells bears more resemblance to Piezo2: It decays with fast kinetics (τdecay-80 mV ~ 12 ms) and slightly, but significantly, decelerates with depolarization. However, because the rate of Piezo2 decay can be influenced by cellular factors, whether lamellar MA currents reported here are mediated by Piezo2 with modified function (4446), or by other proteins (4750), remains to be determined. Our transcriptome analysis of duck bill skin revealed expression of a number of known and putative mechanotransducing ion channels, including Piezo1, Piezo2, transmembrane channel-like protein 2 (TMC2), and transmembrane protein 63 (Tmem63) (fig. S6).

The identification of active touch detection in lamellar cells within Pacinian and Meissner corpuscles suggests that their function extends beyond passive structural support for the neuronal afferent. That removal of the layers surrounding the afferent ending in Pacinian corpuscles converts neuronal firing from rapidly to slowly adapting has long served as evidence that lamellar cells form a passive mechanical filter that prevent static stimuli from reaching the afferent (13). By inference, a similar role has been attributed to the interdigitating protrusions formed between lamellar cells and the neuron in Meissner corpuscles. Although duck Meissner corpuscles display rapidly adapting firing like their mammalian counterparts and have similar frequency tuning characteristics, their lamellar cells form only minimal interdigitations with the neuron. This suggests that extensive mechanical layers around the neuron may be important but not the only prerequisite for rapid adaptation. We instead propose that lamellar cells play an active role in shaping the rapid adaptation of afferent firing, a process that endows Pacinian and Meissner corpuscles with exquisite sensitivity to transient pressure and vibration.

Both types of corpuscle contain molecular components of synaptic machinery (1416). Consistently, our electron microscopic analysis revealed the presence of dense core vesicles in duck Meissner lamellar cells. This raises the possibility that mechanically evoked excitation of lamellar cells triggers the release of neuropeptides or other signaling molecules to shape afferent response. This release could either potentiate or inhibit afferent firing, serving as a fine-tuning mechanism for detection and transmission of mechanosensory signals.

MATERIALS AND METHODS

Animals

Experiments with Pekin duck embryos (Anas platyrhynchos domesticus) were approved by and performed in accordance with guidelines of the Institutional Animal Case and Use Committee of Yale University (protocol 2018-11526).

Preparation of duck bill skin

Pacinian and Meissner corpuscles acquire functionality several days before hatching and become capable of producing a rapidly adapting discharge in the innervating mechanoreceptor in response to touch as early as embryonic day 24 (E24) to E26, similar to corpuscles from adult animals (1921). A patch of skin (~5 mm by 10 mm) from E24 to E26 duck embryo was peeled from the dorsal surface of the upper bill, and the epidermis was mechanically removed to expose Pacinian and Meissner corpuscles. Skin was incubated in collagenase P (2 mg/ml; Roche) in Krebs solution (117 mM NaCl, 3.5 mM KCl, 2.5 mM CaCl2, 1.2 mM MgCl2, 1.2 mM NaH2PO4, 25 mM NaHCO3, and 11 mM glucose, saturated with 95% O2 and 5% CO2 to pH 7.3–7.4 at 22°C) for 20 to 25 min, washed three times with Krebs, and imaged external side up on an Olympus BX51WI upright microscope equipped with an ORCA-Flash 2.8 camera (Hamamatsu).

Patch-clamp electrophysiology of lamellar cells

Recordings were carried out at room temperature using a MultiClamp 700B amplifier and digitized using a Digidata 1550 (Molecular Devices). Patch pipettes were pulled using a P-1000 puller (Sutter Instruments) from 1.5-mm borosilicate glass with a tip resistance of 1.5 to 3 megohms.

Voltage-clamp recordings were acquired in the whole-cell mode using pCLAMP 10 software, sampled at 20 kHz, and low-pass filtered at 10 kHz. Voltage-clamp experiments were recorded from a holding potential of −80 mV, using the following solutions: Internal-Cs: 133 mM CsCl, 5 mM EGTA, 1 mM CaCl2, 1 mM MgCl2, 10 mM Hepes, 4 mM Mg-ATP (adenosine triphosphate), and 0.4 Na2-GTP (guanosine triphosphate; pH 7.3) with CsOH; Internal-K: 135 mM K-gluconate, 5 mM KCl, 0.5 mM CaCl2, 2 mM MgCl2, 5 mM EGTA, 5 mM Hepes, 5 mM Na2ATP, and 0.5 mM GTP-tris (pH 7.3) with KOH; Bath Ringer: 140 mM NaCl, 5 mM KCl, 10 mM Hepes, 2.5 mM CaCl2, 1 mM MgCl2, and 10 mM glucose (pH 7.4) with NaOH. Voltage-gated potassium currents were recorded using Internal-K and Bath Ringer. Currents were elicited by 500-ms voltage steps from −100 mV, in 10-mV increments. Voltage-gated sodium and calcium (Cav) currents were recorded using Internal-Cs and Bath Ringer supplemented or not with 300 μM CdCl2 or 20 μM CaCl2. Currents were elicited using 500-ms voltage steps from −100 mV, in 10-mV increments. Each voltage step was proceeded by a 500-ms hyperpolarizing step to −120 mV to remove channel inactivation. Leak current was subtracted using the P/4 protocol. Series resistance was compensated at 50%. Peak Cav currents were converted to conductance using the equation G = I/(VmErev), where G is the conductance, I is the peak Cav current, Vm is the membrane potential, and Erev is the reversal potential. The conductance data were fit with the modified Boltzmann equation, G = Gmin + (GmaxGmin)/(1 + exp^([V1/2Vm]/k)), where Gmin and Gmax are minimal and maximal conductance, respectively, Vm is the voltage, V1/2 is the voltage at which the channels reached 50% of their maximal conductance, and k is the slope of the curve.

MA currents were recorded in Internal-Cs and Bath Ringer at a −60-mV holding potential. After whole-cell formation, a blunt glass probe (2 to 4 μm at the tip) mounted on a piezoelectric-driven actuator (Physik Instrumente GmbH) was positioned to touch the corpuscle at the side opposite to the patch pipette. The probe mounted was moved at a velocity of 800 μm/s toward the corpuscle in 1-μm increments, held in position for 150 ms and then retracted at the same velocity.

To visualize lamellar cells, Lucifer yellow was added to internal solution at concentration of 2 mM. Resting membrane potentials were measured upon break-in using Internal-K and Bath Ringer. Voltage-clamp experiments and resting membrane potential measurements were corrected offline for liquid junction potential calculated in Clampex 10.7.

Current-clamp experiments were recorded using Internal-K and Krebs in the bath. Recordings were started 2 min after break-in to stabilize the action potential firing. Changes in membrane potential were recorded in response to 1-s current pulses from a 0- to −30-pA holding, in 10-pA increments. Current-clamp experiments were not corrected for liquid junction potential. For pharmacological experiments, bath solution was supplemented with the following: 300 μM CdCl2, 20 μM CaCl2, 10 μM felodipine (Abcam), a mix of 10 μM nimodipine and 5 μM isradipine (Alomone), 10 μM nifedipine (Alomone), agatoxin mix (1 μM ω-agatoxin IVA and 1 μM ω-agatoxin TK from Alomone), conotoxin mix (5 μM ω-conotoxin CnVIIA, 10 nM ω-conotoxin CVIB, 10 nM ω-conotoxin CVIE, 1 μM ω-conotoxin MVIIC, and 1 μM ω-conotoxin MVIID from Alomone), 1 μM SNX-482 (from Alomone or Peptides International), 5 μM mibefradil*2HCl, 200 nM kurtoxin (Alomone), and 200 μM tetrodotoxin citrate (Tocris). Paired recordings were performed 1 to 10 min after the addition of small-molecule drugs or 1 to 20 min after the addition of peptide toxins. The frequency distribution of mechanically evoked action potentials was obtained by binning the number of action potentials by 9-ms intervals, followed by fitting the resulting data to the single exponential decay equation Y = (Yo − Y) × exp^(−tdecay) + Y, where t is the time from the beginning of current injection, Yo and Y are the percentages of the number of mechanically evoked action potentials at t = 0 and at infinity, respectively, and τdecay is the decay constant.

Preparation of trigeminal neurons

Trigeminal neurons from embryonic duck (E24 to E26) were acutely dissociated as previously described (19, 26). Dissected duck trigeminal ganglia were chopped with scissors in 500 μl of ice-cold Hanks’ balanced salt solution (HBSS), dissociated by adding 500 μl of collagenase P (2 mg/ml; Roche) dissolved in HBSS, and incubated for 15 min at 37°C, followed by incubation in 500 μl of 0.25% trypsin-EDTA for 10 min at 37°C. The trypsin was then removed, and the residual trypsin was quenched by adding 750 μl of prewarmed DMEM+ medium [Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin, and 2 mM glutamine]. Cells were triturated gently with plastic P1000 and P200 pipettes and collected by centrifugation for 3 min at 100g. Cells were resuspended in DMEM+ medium and plated onto the Matrigel (BD Bioscience, Billerica, MA)–precoated coverslips in a 12-well cell culture plate. DMEM+ medium (0.5 ml) was added into each well following incubation at 37°C in 5% CO2 for 30 to 45 min. MA current measurements were performed within 48 hours after plating.

Patch-clamp electrophysiology of trigeminal neurons

Voltage-clamp recordings were acquired in the whole-cell mode using pCLAMP software using 1.5-mm borosilicate glass with a tip resistance of 1.5 to 5 megohms. Recordings were performed in Bath Ringer, sampled at 20 kHz, and low-pass filtered at 2 to 10 kHz. Internal solution contained the following: 130 mM K-methanesulfonate, 20 mM KCl, 1 mM MgCl2, 10 mM Hepes, 3 mM Na2ATP, 0.06 mM Na2GTP, and 0.2 mM EGTA (pH 7.3) with KOH (final [K+] = 150.5 mM). Before mechanical stimulation, current was injected in current-clamp mode to elicit neuronal firing to ensure neuronal fitness. Mechanical stimulation was performed using a blunt glass probe positioned at 32° to 55° relative to the cell, as described above for corpuscles. Membrane potential was clamped at −60 mV. Neurons with MA current were classified on the basis of the rate of MA current inactivation (τdecay) as fast inactivating (τdecay < 10 ms), intermediately inactivating (τdecay = 10 to 30 ms), and slow inactivating (τdecay > 30 ms), as previously described (26): The decaying component of MA current was fit to the single exponential decay equation I =I × exp^(− t/τdecay), where ∆I is the difference between peak MA current and baseline, t is the time from the peak current (the start of the fit), and τdecay is the decay constant. Resultant τdecay for each neuron represent an average from traces with the top 75% of MA amplitude (35). MA current rise time (τrise) was quantified by fitting a single exponential function in a similar manner as for τdecay.

RNA sequencing

Total RNA was isolated from duck bill skin using the TRIzol reagent (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions. RNA integrity was assessed on the basis of RNA integrity number (RIN) values obtained with Agilent Bioanalyzer. Library preparation and sequencing were carried out at the Yale Center for Genome Analysis. mRNA was purified from ~200 ng of total RNA with oligo-dT beads. Strand-specific sequencing libraries were prepared using the KAPA mRNA HyperPrep Kit (Roche Sequencing Solutions, Pleasanton, CA). Libraries were sequenced on Illumina NovaSeq sequencer in the 100–base pair paired-end sequencing mode according to the manufacturer’s protocols with multiple samples pooled per lane. A total of ~50 million to 69 million sequencing read pairs per sample were obtained. The sequencing data were processed on the Yale High Performance Computing cluster. Raw sequencing reads were filtered and trimmed to retain high-quality reads using Trimmomatic v0.36 with default parameters. Filtered high-quality reads from all samples were aligned to duck reference genome using the STAR aligner v2.5.4b with default parameters. The reference genome (A. platyrhynchos, BGI_duck_1.0) and gene annotation (National Center for Biotechnology Information release 102) were obtained from the National Center for Biotechnology Information (accessed on 5 August 2018). The gene annotation was filtered to include only protein-coding genes. Aligned reads were counted by featureCounts program within the Subread package v1.6.2 with default parameters. Raw read counts were processed and converted to “mRNA fragments per kilobase of exon per million mapped fragments” values by edgeR v3.22.3. The RNA sequencing data were deposited to the Gene Expression Omnibus (accession number: GSE155529).

Calcium imaging

Live-cell ratiometric calcium imaging was performed on duck bill skin patches at room temperature using an Axio Observer Z1 inverted microscope (Zeiss) equipped with an ORCA-Flash 4.0 camera (Hamamatsu) using MetaFluor software (Molecular Devices). After collagenase treatment, skin patch was loaded with 10 mM Fura-2 AM (Thermo Fisher Scientific) and 0.02% Pluronic F-127 in Ringer solution for 30 min at room temperature and washed three times with Ringer solution. The skin was then visualized and exposed to a high-K+ solution, containing the following: 10 mM NaCl, 135 mM KCl, 2 mM CaCl2, 2 mM MgCl2, 10 mM glucose, and 10 Hepes (pH 7.4; with KOH). Background signal was quantified from skin areas devoid of corpuscles.

Electron microscopy

Freshly peeled duck bill skin was fixed in Karnovsky fixative at 4°C for 1 hour, washed in 0.1 M sodium cacodylate buffer (pH 7.4), postfixed in 1% osmium tetroxide for 1 hour in the dark on ice. The tissue was stained in Kellenberger solution for 1 hour at room temperature after washing in distilled water, dehydrated in a series of alcohols and propylene oxide, then embedded in EMbed 812, and polymerized overnight at 60°C. All solutions were supplied by Electron Microscopy Sciences, Hatfield, PA. Ultrathin sections were obtained on a Leica Ultracut UCT ultramicrotome at 70 nm, stained in 1.5% aqueous uranyl acetate and Reynolds’ lead stains, and imaged on an FEI Tecnai G2 Spirit BioTWIN electron microscope.

Quantification and statistical analysis

Electrophysiological data from corpuscles and trigeminal neurons were obtained from skin preparations from at least three animals. All measurements were taken from distinct samples. Data were analyzed and plotted using GraphPad Prism 8.4.3 (GraphPad Software Inc.) and expressed as means ± SEM or as individual points. Statistical tests were chosen on the basis of experimental setup, sample size, and normality of distribution, as determined by the Kolmogorov-Smirnov test, and are specified in the figure legends. Adjustments for multiple comparisons were performed where appropriate.

Supplementary Material

http://advances.sciencemag.org/cgi/content/full/6/51/eabe6393/DC1
Adobe PDF - abe6393_SM.pdf
abe6393_SM.pdf (1.1MB, pdf)
Lamellar cells in Pacinian and Meisner corpuscles are touch sensors

Acknowledgments

We thank S. Mentone for electron microscopy imaging, R. Dashkin for help with data visualization, and members of the Bagriantsev and Gracheva laboratories for contributions throughout the project. Funding: This study was supported by NSF grant 1923127 and NIH grant 1R01NS097547-01A1 (to S.N.B.) and by NSF grants 1754286 and 2015622 and NIH grant 1R01NS091300-01A1 (to E.O.G.). E.R.S. was supported by a postdoctoral fellowship from the Arnold and Mabel Beckman Foundation. Author contributions: E.O.G. and S.N.B. conceived the project. Y.A.N. and E.O.G. developed the skin preparation. Y.A.N. performed electrophysiological and calcium imaging recordings from corpuscles. V.V.F. performed transcriptomic analysis. E.O.A. performed electrophysiological recordings from trigeminal neurons. E.R.S. performed pilot electrophysiological experiments with duck bill skin. Y.A.N., E.O.G., and S.N.B. wrote the paper. Competing interests: The authors declare that they have no competing interests. Data and material availability: All data needed to evaluate the conclusions in the paper are present in the paper and/or the Supplementary Materials. The RNA sequencing data were deposited to the Gene Expression Omnibus (accession number GSE155529). Additional data related to this paper may be requested from the authors.

SUPPLEMENTARY MATERIALS

Supplementary material for this article is available at http://advances.sciencemag.org/cgi/content/full/6/51/eabe6393/DC1

View/request a protocol for this paper from Bio-protocol.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

http://advances.sciencemag.org/cgi/content/full/6/51/eabe6393/DC1
Adobe PDF - abe6393_SM.pdf
abe6393_SM.pdf (1.1MB, pdf)
Lamellar cells in Pacinian and Meisner corpuscles are touch sensors

Supplementary material for this article is available at http://advances.sciencemag.org/cgi/content/full/6/51/eabe6393/DC1


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