The complete mitochondrial genome of Lunella coronata correensis

Abstract

The complete mitochondrial (mt) genome of Lunella coronata correensis was determined using genome walking techniques in this study. The total length of the mt genome sequence of L. coronata correensis was 16,551 bp, including 13 protein-coding genes, 23 transfer RNA genes, and 2 ribosomal RNA genes. The overall composition of the mitogenome was estimated to be 33.1% for A, 35.3% for T, 13.7% for C, and 19.9% for G, respectively, indicating that an A + T (68.4%)-rich feature occurs in the L. coronata correensis mitogenome. The phylogenetic relationships of 12 mollusk species were constructed based on the complete mtDNA sequences by the neighbor-joining method using MEGA version 7.0 and DNAMAN version 6.0 software.

The Lunella coronata correensis belongs to the family Turbinidae, and could be found in northern parts Yellow Sea and Bohai Sea of China. The population of wild L. coronata correensis is very small, and the research about L. coronata correensis is rare. In order to provide some biological data for L. coronata correensis, it is necessary to carry out some wild resource investigation and germplasm analysis. In this study, we report the complete sequence of mitochondrial (mt) genome for L. coronata correensis (GenBank accession no. MN604179). The findings will provide useful information for further studies on population genetics, phylogenetic construction and other relevant studies in L. coronata correensis.

One L. coronata correensis individual was collected from Dalian Sea area, Liaoning Province, China (39°11′23″N, 124°47′24″E). The total genomic DNA was extracted from foot muscle by a modification of standard phenol-chloroform procedure. The complete mitogenome of L. coronata correensis was sequenced by primer walking. The gene annotation was performed following the methods described by Hao et al. (2016) and Online software (GeneMarker, tRNAscan, ORFfinder). At present, The L. coronata correensis specimens were stored in the Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, PR China, Dalian Ocean University, Dalian, 116023, Liaoning, China. The specimens were numbered DLOU -KLM-SSH06 to DLOU-KLM-SSH09.

The total length of the hybrid of L. coronata correensis mt genome was 16,551 bp, with the base composition of 33.1% A, 35.3% T, 13.7% C, and 19.9% G. It comprised 2 ribosomal RNA genes, 13 protein-coding genes, and 23 transfer RNA genes. All the mitogenome genes were encoded on the light strand except for 11 tRNA genes (tRNA-Asp, tRNA-Glu, tRNA-Gly, tRNA-Ala, tRNA-Arg, tRNA-Asn, tRNA-Ser, tRNA-Ile, tRNA-(Lys/Asn), and tRNA-Thr).

The phylogenetic analysis showed that the complete mt sequence of L. coronata correensis was phylogenetically closer to Bolma rugosa by 71.76% bootstrap support, and formed the Turbinidae clade (Figure 1). Our result was consistent with the previous researches, both in traditional morphological and molecular-based phylogeny studies. We expect that the present result can contribute to construct molecular identification of this species and be helpful to explore the phylogeny of Muricidae.

Figure 1.

Consensus neighbor-joining tree based on the complete mitochondrial sequence of L. coronata correensis and other 11 mollusk species. The phylogenetic tree was constructed using MEGA 7.0 and DNAMAN 6.0 software by the neighbor-joining method. The numbers at the tree nodes indicate the percentage of bootstrapping after 1000 replicates.

Funding Statement

This study was supported by funds from Natural Science Foundation of Liaoning Province [201602099] and Dalian Science and Technology Innovation Foundation Project [2018J11CY029].

Disclosure statement

No potential conflict of interest was reported by the authors.