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. 2020 Jan 27;5(1):889–890. doi: 10.1080/23802359.2020.1718028

The complete chloroplast genome of Bougainvillea glabra

Meng He 1, Xihao Wang 1, Yangtao Zhuang 1, Xiang Jin 1,✉
PMCID: PMC7748614  PMID: 33366799

Abstract

Bougainvillea glabra is one of the most popular ornamental and landscaping plants planted in tropical and subtropical regions. The brightly colored bracts, long florescence and strong stress resistance make B. glabra perfect ornamental horticulture plant. Bougainvillea plants have been frequently hybridized, resulting in more than 400 varieties. To investigate the chloroplast genome will help us to understand the biological diversity and stress resistance of Bougainvillea plants better. Here, we report the complete chloroplast genome of B. glabra, which is 154,542 bp in length, including a large single copy (LSC) region of 85,695 bp and a small single copy (SSC) region of 18,077 bp, separated by a pair of identical inverted repeat regions (IRs) of 25,385 bp each. A total of 128 genes were identified, including 83 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis based on 12 chloroplast genomes showed that B. glabra, accompanied with its sister species B. spectabilis, formed a base clade in Nyctaginaceae which was close to Pisonia aculeata. This study will be helpful for better understanding of the genetic diversity and stress resistance of Bougainvillea plants.

Keywords: Bougainvillea glabra, chloroplast genome, biological diversity, phylogeny

The genus Bougainvillea, which is originated from Amazonian rainforests in South America, contains important ornamental and landscaping plants that are widely distributed in tropical and subtropical areas (Abarca-Vargas and Petricevich 2018). Only four species out of 18 in this genus were well studied: B. buttiana, B. glabra, B. spectabilis, and B. peruviana (Zhou et al. 2011). In Africa, the extracts of B. glabra are used to treat intestinal disorder, inflammation related cases and pain reliever (Ogunwande et al. 2019). In addition, B. glabra has gorgeous colored bracts, long florescence and strong stress resistance, which make it high valuable in urban landscape greening and ornamental horticulture (Tsai et al. 2005). Due to its commercialization, the Bougainvillea plants were hybridized frequently, resulting in more than 400 varieties, the genetic background of which are complex (Zhao et al. 2019). Chloroplasts are important for plant interactions with the environment (heat, drought, salt, light, etc.) and chloroplast genome sequence is also helpful to understand biological diversity (Daniell et al. 2016). Here, we report the complete chloroplast genome of B. glabra, which will be useful to understand the biological diversity and stress resistance of Bougainvillea species, accompanied with recently reported B. spectabilis chloroplast genome (Wang et al. 2019; Yao et al. 2019).

The sample of B. glabra was collected from the botanical garden of Hainan Normal University, Haikou, China (100°20′35.63″E, 19°59′46.72″N). The voucher specimen is stored in the herbarium of Hainan Normal University (specimen no. 19HNNU0722). Chloroplasts were isolated from 30 g of fresh leaves by gradient centrifugation on Percoll. Then, chloroplast genomic DNA were extracted using SDS method. A 350 bp DNA library was constructed and then sequenced PE-150 bp on Illumina NovaSeq 6000 platform. A total of 2.3 GB clean data were generated. Complete chloroplast genome of B. glabra was assembled by SOAPdenovo 2.04. Assembled chloroplast genome was annotated using online tool CPGAVAS (http://www.herbalgenomics.org/cpgavas/) (Shi et al. 2019).

The complete chloroplast genome of B. glabra (GenBank Accession MN888961) has a circular DNA of 154,542 bp with GC content of 35.19%. It has a large single copy (LSC) region of 85,695 bp and a small single copy (SSC) region of 18,077 bp, separated by a pair of identical inverted repeat regions (IRs) of 25,385 bp each. In addition, we annotated 128 genes, including 83 protein-coding genes, 37 tRNA genes, and 8 rRNA genes.

To reveal the phylogenetic position of B. glabra, a maximum-likelihood (ML) phylogenetic tree was constructed using 12 complete chloroplast genome sequences, including B. glabra, 9 Caryophyllales species and two Buxaceae outgroups (Buxus microphylla and Pachysandra terminalis). All sequences were download from GenBank under corresponding accessions and were aligned using MAFFT v7.455 (Katoh and Standley 2013). The ML tree was produced by RAxML (Stamatakis 2014) using 1000 bootstrap replicates. The phylogenetic tree showed that B. glabra, accompanied with B. spectabilis, formed a base clade in Nyctaginaceae which was close to Pisonia aculeata (Figure 1). The complete chloroplast genome of B. glabra will help to study the genetic diversity and stress resistance of Bougainvillea plants.

Figure 1.

Figure 1.

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Phylogenetic analysis of 10 species from Caryophyllales and two outgroups based on complete chloroplast genome sequences by RAxML. Bootstrap was set to 1000.

Funding Statement

This work was supported by the Education Department of Hainan Province [project number: Hnky2019-26]; Undergraduates Training Program for Innovation and Entrepreneurship of Hainan Province [project number: S201911658016].

Disclosure statement

No potential conflict of interest was reported by the authors.

References

  1. Abarca-Vargas R, Petricevich VL. 2018. Bougainvillea genus: a review on phytochemistry, pharmacology, and toxicology. Evid Based Complement Alternat Med. 2018:9070927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Daniell H, Lin CS, Yu M, Chang WJ. 2016. Chloroplast genomes: diversity, evolution, and applications in genetic engineering. Genome Biol. 17(1):134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Katoh K, Standley DM. 2013. MAFFT: multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol. 30(4):772–780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ogunwande IA, Avoseh ON, Olasunkanmi KN, Lawal OA, Ascrizzi R, Flamini G. 2019. Chemical composition, anti-nociceptive and anti-inflammatory activities of essential oil of Bougainvillea glabra. J Ethnopharmacol. 232:188–192. [DOI] [PubMed] [Google Scholar]
  5. Shi L, Chen H, Jiang M, Wang L, Wu X, Huang L, Liu C. 2019. CPGAVAS2, an integrated plastome sequence annotator and analyzer. Nucleic Acids Res. 47(W1):W65–W73. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Stamatakis A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics. 30(9):1312–1313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Tsai CH, Su HJ, Liao YC, Hung TH. 2005. First report of Bougainvillea spectabilis chlorotic vein-banding virus infecting Bougainvillea plants in Taiwan. Plant Dis. 89(12):1363–1363. [DOI] [PubMed] [Google Scholar]
  8. Wang N, Qiu MY, Yang Y, Li JW, Zou XX. 2019. Complete chloroplast genome sequence of Bougainvillea spectabilis (Nyctaginaceae). Mitochondrial DNA Part B. 4(2):4010–4011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Yao G, Jin JJ, Li HT, Yang JB, Mandala VS, Croley M, Mostow R, Douglas NA, Chase MW, Christenhusz MJM, et al. 2019. Plastid phylogenomic insights into the evolution of Caryophyllales. Mol Phylogenet Evol. 134:74–86. [DOI] [PubMed] [Google Scholar]
  10. Zhao T, Chang SX, Leng QY, Xu SS, Yin JM, Niu JH. 2019. Development of SSR molecular markers based on transcriptome sequencing of Bougainvillea. Mol Plant Breed. 17(13):4331–4341. [Google Scholar]
  11. Zhou Q, Huang KF, Ding YL, Guo HZ. 2011. Investigation and taxonomic identification on introduced ornamental varieties in Bougainvillea in China. Acta Agri Jiangxi. 23(5):53–56. [Google Scholar]

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