Abstract
The complete mitogenome sequence of an Asian longicorn beetle Dorysthenes granulosus (Thomson 1860) was sequenced. The 15,858 bp long genome has the standard metazoan complement of 38 genes. These genes contain 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 control region. The nucleotide composition of the D. granulosus mitogenome was A: 39.5%, T: 31.7%, G: 10.9%, C: 17.9%. The A＋T content was 71.2%, showing strong AT skew. Phylogenetic analysis indicated that D. granulosus have sister relationship with Dorysthenes paradoxus.
Keywords: Longicorn beetle, Dorysthenes granulosus, Prioninae, mitochondrial genome
Introduction
Asian longicorn beetle Dorysthenes granulosus (Thomson) (Coleoptera: Cerambycidae: Prioninae), an underground pest that mainly damages sugarcane and cassava (Yu et al. 2006; Chen et al. 2012), is found in southern China, India, Myanmar and Thailand, spreading rapidly in the Chinese provinces of Hainan, Guangxi, Guangdong and Guizhou (Wickham et al. 2016). Newly hatched larvae bite the tender roots of sugarcane, and gradually eat into the stems after growing up, forming hollow sugarcane, seriously affecting the yield of sugarcane (Gong et al. 2008). Mitochondrial genome sequences are essential to a deeper understanding of the evolution of Cerambycidae and identify larva species (Wang et al. 2019). Here, the complete mitochondrial DNA (mtDNA) genome of D. granulosus was elucidated which has not been reported before.
In this study, specimens of D. granulosus were collected from the Qingxiu Mountain (22°47′N, 108°23′E) of Nanning City (Guangxi Province, China). The total genomic DNA was extracted following the modified CTAB DNA extraction protocol and stored at Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety (The city of Nanning, China) with sample number of FDSW190073798. Then library was constructed and pair-end was sequenced (2*150 bp) with HiSeq (Illumina, San Diego, CA). Via SPAdes (version 3.9), approximately 15.33 G of raw data and 15.26 G of clean data were obtained for sequence assembly (Bankevich et al. 2012).
The complete mitochondrial genome of D. granulosus is a closed circular molecule 15,858 bp in length (GenBank accession number MN829437) and constitutive of 38 genes. These genes contain 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and 1 control region. The nucleotide composition of the D. granulosus mitogenome was A (39.5%), T (31.7%), G (10.9%), C (17.9%). The A＋T content was 71.2%, showing strong AT skew.
The phylogenetic relationship among Prioninae, Cerambycinae and Lamiinae species by Neighbor-Joining method with 1000 bootstrap replicates (Sudhir et al. 2016) were estimated using the Molecular Evolutionary Genetics Analysis Version 7.0 (MEGA7.0). The results showed that mtDNA of D. granulosus had a close relationship with that of D. paradoxus (Liu et al. 2018) (Figure 1).
Figure 1.
Neighbor-Joining phylogenetic tree of Dorysthenes granulosus and other Prioninae, Cerambycinae and Lamiinae beetles. The complete mitochondrial genome was downloaded from GenBank and the phylogenic tree was constructed by Neighbor-Joining method with 1000 bootstrap replicates were estimated using the MEGA7.0. The results showed that mtDNA of D. granulosus had a close relationship with that of D. paradoxus (Figure 1).
Acknowledgements
We appreciated the sequencing service provided by Huitong biotechnology Co. Ltd (Shenzhen, China).
Disclosure statement
The authors declare no conflict of interest. The authors alone are responsible for the content and writing of the paper.
References
- Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, et al. 2012. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. Comput Biol. 19(5):455–477. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chen Q, Lu FP, Lu H, Xu XL, Jing FL, Xiao ZY, Li ZG, Liao Q, Li KM. 2012. Occurrence regularity and control technology of cassava major soil insects grub and Dorysthenes granulosus. Chin J Trop Crop. 33(2):332–337. [Google Scholar]
- Gong HL, An YX, Guan CX, Yang JX, Lin MJ, He ZY, Liu YC, Xu HL, Sun DL. 2008. The feature and damage of Dorysthenes granulosus (Thomson) to sugarcane in China and its integrated management. Sugarcane and Canesugar. (5):1–5. [Google Scholar]
- Liu YQ, Chen DB, Liu HH, Hu HL, Bian HX, Zhang RS, Yang RS, Jiang XF, Shi SL. 2018. The complete mitochondrial genome of the longhorn beetle Dorysthenes paradoxus (Coleoptera: Cerambycidae: Prionini) and the implication for the phylogenetic relationships of the cerambycidae species. J Insect Sci. 18(2):21;1–8 [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sudhir K, Glen S, Koichiro T. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 33(7):1870–1874. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang XY, Zheng XL, Lu W. 2019. The complete mitochondrial genome of an Asian longicorn beetle Glenea cantor (Coleoptera: Cerambycidae: Lamiinae). Mitochondrial DNA Part B. 4(2):2906–2907. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wickham J D, Lu W, Jin T, Peng Z, Guo D, Millar J G, Hanks L M, Chen Y. 2016. Prionic acid: an effective sex attractant for an important pest of sugarcane, Dorysthenes granulosus (Coleoptera: Cerambycidae: Prioninae. J Econ Entomol. 109(1):484–486. [DOI] [PubMed] [Google Scholar]
- Yu YH, Zeng T, Wei DW, Zeng XR, Li LF, Wang ZY. 2006. Damage status of Dorysthenes granulosus (Thomson) and its control strategies in Guangxi sugarcane region. J Southern Agric. (5):545–547. [Google Scholar]