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. 2020 Dec 15;6(12):e05639. doi: 10.1016/j.heliyon.2020.e05639

Figure 7.

Figure 7

Linalool and 1,8-cineole increased simvastatin-mediated cell cycle arrest and apoptosis in A549 cells. Cells were exposed to ethanol 0.2% (Control), linalool 1.0 mM (LN 1), 1,8-cineole 4.0 mM (CN 4) and simvastatin 10 μM (SV) alone or each monoterpene combined with simvastatin (LN + SV, CN + SV). (A) The cell cycle was analyzed following 24 h treatments. Scale bar, 10 μm. (B) Representative images of TUNEL assay performed after exposing A549 cells to the treatments for 48 h. (C) Around a total of ten to fifteen fields (200–500 cells/field) were evaluated and TUNEL-positive cells were quantified. Data are expressed as mean ± SD. Means without the same letter are different (p < 0.05). (D) Representative western blot of PARP and cleaved-PARP. GAPDH was used as a loading control.