Abstract
Background
Plasminogen activator inhibitor-1 (PAI-1) is a key molecule residing at the nexus between thrombosis and inflammatory processes. Recently, PAI-1 and its gene expression have emerged as a potential candidate for autoimmune disorders such as SLE.
Objective
To investigate whether the PAI-1 4G/5G polymorphism at position −675 could be a genetic marker for susceptibility to childhood-onset SLE and development of lupus nephritis among Egyptian children and adolescents.
Methods
Three hundred fifty patients diagnosed with childhood-onset SLE and 350 well-matched healthy controls were included in this multi-center study. All subjects were genotyped for the PAI-1 promoter 4G/5G polymorphism at position −675 using PCR– restriction fragment length polymorphism (RFLP). Serum PAI-1 levels were measured by ELISA.
Results
The PAI-1 (- 675) 4G/4G genotype was more represented in c-SLE patients, as compared to the control group (38% vs 23%; OR =2.7; [95% CI: 1.47–2.9]; P < 0.001). Patients carrying the PAI-1 4G/4G genotype or 4G allele were more likely to develop lupus nephritis (OR: 3.38; [95% CI: 1.9–5.9]; P <0.001, for the 4G/4G genotype and OR: 2.6; [95% CI: 1.85–3.67]; for the 4G allele; P < 0.01). The PAI-1 4G/4G genotype was associated with higher PAI-1 serum concentrations (mean; 86.6±22.7 ng/mL) as compared to those with a 4G/5G genotype (mean; 48.3±16.5 ng/mL) and the lowest for the 5G/5G genotype (mean; 34.7±11.4 ng/mL); P = 0.004.
Conclusion
The PAI-1 4G/5G polymorphism may confer susceptibility to childhood-onset SLE and development of lupus nephritis among Egyptian children and adolescents. Moreover, the PAI-1 4G/4G genotype and 4G allele were associated with higher PAI-1 serum levels and higher disease activity scores.
Keywords: plasminogen activator inhibitor-1, PAI-1, gene polymorphism, SLE, children, adolescents
Introduction
Childhood-onset systemic lupus erythematosus (cSLE) is a multifaceted autoimmune and inflammatory disease, accounts for about one in every five cases of SLE.1 cSLE diverse presentation range from mild cutaneous and articular involvement through nephritis, hematological or neuropsychiatric disorders to end-stage renal disease [ESRD] and catastrophic thrombosis.2 More than 60% of childhood-onset cases develop lupus nephritis (LN) with approximately one-fourth of them proceed to ESRD.3 cSLE hallmark is multiple pathogenic autoantibody production with immune‐mediated inflammation and tissue injury.4 The disruption of dynamic balance between the coagulation cascade and fibrinolysis pathway have been linked with an increased tendency to glomerular microthrombosis in a subset of patients with LN.5,6 Recent studies have focused on molecules residing at the nexus between thrombosis and inflammatory processes as potential candidate for SLE susceptibility and phenotype presentation or disease progression.7
Among these molecules, plasminogen activator inhibitor-1 (PAI-1) is a member of the serpin protease family and serves as the major inhibitor of endogenous fibrinolysis pathway. It is mainly synthesized by endothelial cells, adipocytes and platelets.8 PAI-1 can bind to and inhibit both tissue-type (t-PA) and urokinase-type (u-PA) plasminogen activators. Thus, PAI-1 reduces the conversion of plasminogen to plasmin, the main enzyme involved in fibrinolysis.9
A more recent study by Li et al suggested that impaired fibrinolysis contributes to a hypercoagulability state and microthrombotic events in Chinese pediatric cohort with SLE as demonstrated by increased levels of PAI-1 and decreased levels of t-PA.10
A recent meta-analysis by Bae et al reported significant up-regulation of circulating PAI-1 levels in multi-ethnic cohorts with adult-onset SLE.11 In murine lupus models, PAI-1 was highly expressed throughout its inflamed kidneys and the level of PAI-1 in renal tissues was correlated with the severity of lupus nephritis.12 Despite these reports, a genetic approach could be the only way to explore the potential role of PAI-1 in etiopathogenesis of childhood-onset SLE and lupus nephritis.
Human PAI-1 is encoded by the SERPINE1 gene mapped to chromosome 7q21.3-q22.1 in which several polymorphisms have been discovered.13 A functional insertion/deletion single nucleotide polymorphism (SNP) containing either four or five guanine bases (4G/5G) in the PAI-1 gene promoter at the −675 position (rs1799889) was reported to modulate PAI-1 mRNA transcriptional activity and expression level.14 In vitro studies demonstrated that the 4G allele produces 6 times more PAI-1 mRNA expression than the 5G allele in response to interleukin (IL)-1B.15
In Caucasian population, the PAI-1 4G allele was found to be associated with approximately 25% higher plasma PAI-1 concentrations16 and may represent a susceptibility allele for autoimmune disorders including SLE.11 Given the sparse data on PAI-1 in the context of pediatric lupus, we aimed to investigate whether the PAI-1 (4G/5G) promoter polymorphism at the −675 position could be a genetic marker for cSLE susceptibility or development of lupus nephritis in Egyptian children and adolescents. In addition, we assessed variations in PAI-1 serum levels related to the PAI-1 (4G/5G) polymorphism.
Methods
This multicenter case-control study was carried out at Helwan, Zagazig, Cairo, and Sohag University hospitals and outpatient clinics at the same hospitals. The study protocol was approved by medical ethics committee at Helwan, Zagazig, Cairo and Sohag Universities, Egypt. Written informed parental consent was provided for all participants conformed to the Declaration of Helsinki.
Three hundred fifty unrelated patients with onset of SLE before 18 years of age who fulfilled at least 4 of revised American College of Rheumatology SLE classification criteria,17 were enrolled from April 2017 to May 2020. All patients were newly diagnosed SLE cases. Detailed demographic data, age at diagnosis of SLE, cumulative clinical features, laboratory findings, and duration of follow-up were recorded. Medications used at the study time were also recorded (intravenous methylprednisolone pulses, prednisone, hydroxychloroquine sulfate (HCQ) and immunosuppressive agents ie, intravenous cyclophosphamide, mycophenolate mofetil, azathioprine, and cyclosporine). Disease activity and flare scoring was calculated according to the revised SLEDAI-2000 (2K) modification.18 Routine laboratory tests were investigated at study entry including complete blood count (CBC), urine analysis, urine protein/creatinine ratio, complements C3 and C4, antinuclear antibodies (ANA), antibodies to double-stranded DNA (anti-ds DNA) titer, anti-phospholipid antibodies, and lupus anticoagulant. Patients with clinically evident LN underwent diagnostic percutaneous ultrasound-guided renal biopsy before the start of treatment. Renal histopathology was classified by expert pathologist (ETE) according to guidelines published by the International Society of Nephrology/Renal Pathology Society for evaluation and scoring of LN.19
Exclusion Criteria
Patients were excluded if they met criteria for other autoimmune disease, renal disease other than lupus-related nephritis, and those on dialysis. Patients diagnosed with anti-phospholipid syndrome, primary hematologic or liver disease, severe infectious illness and any other comorbidity were also excluded.
The control population comprised 350 unrelated healthy children and adolescents, of matched age and gender; who attended for routine checkup at outpatient clinics of the study hospitals. Both patient and control groups belong to the same ethnicity; African-Caucasian. For all subjects, 3 mL whole blood samples were withdrawn at 08.00 A.M. after an overnight fast to avoid the diurnal variation in PAI-1 serum level.20
Serum PAI-1 Assay
Serum PAI-1 concentrations were assayed using Immuno Quantitative ELISA kit (RayBio® Human PAI-1 IQELISA™ Kit, GA 30,092, USA) using a standard curve with detection range: 3.01 pg/mL to 12,500 pg/mL and sensitivity: 3.01 pg/mL.
Genomic DNA Extraction
The Gene JET kit was used to extract the DNA from 200 μL of whole blood (Genomic DNA Purification Mini Kit, Thermo Scientific, USA) and stored at −20° C for genotyping.
Genotyping
We genotyped all subjects for the PAI-1 promoter 4G/5G polymorphism at position – 675 (rs1799889) by PCR– restriction fragment length polymorphism (RFLP) method. The sense primer 5ʹ-CAC AGA GAG AGT CTG GCC ACGT-3ʹ and the antisense primer 5ʹ-CCA ACA GAG GAC TCT TGG TCT-3ʹ were used as described by Rossaak et al.21 The PCR products were a 99 base pair (bp) for the 5G allele and a 98 bp for the 4G allele. The amplified DNA was digested with restriction endonuclease enzyme Bsl I (New England Biolabs) at 37 °C for 90 minutes and then, analyzed by electrophoresis in a 3% agarose gel after staining with ethidium bromide. Digestion fragments of 77 and 22 bp correspond to the (5G/5G); fragment 98 bp corresponds to the (4G/4G); and fragments of 98, 77, 22 bp correspond to the (4G/5G) genotypes; Figure 1.
Figure 1.
Gel electrophoresis of PCR products. Lane 1; 100-bp marker; lane 2, 3 represent the 5G/5G genotype; lane 4, 5 represent the 4G/4G genotype; and lane 6, 7 represent the 4G/5G genotype.
Statistical Analysis
The appropriate sample size was determined using PAWE-3D; together with the specific study design, hypothesis, inheritance model, allele frequency, and allowable error rates; to detect variant allele frequency of 0.05 and genotype relative risk of ≥1.8 at 80% power. The Chi-square (χ2) test was applied to estimate the Hardy-Weinberg equilibrium (HWE) and to compare PAI-1 (4G/5G) genotype frequencies between the patient and control groups. Logistic regression analysis was performed to assess possible associations between PAI-1 (4G/5G) genotypes and lupus nephritis, SLEDAI score and PAI-1 serum levels. Odds ratios and their 95% confidence intervals [OR; 95% CI] were calculated. Continuous parameters were compared with unpaired Student’s t‐test or one‐way analysis of variance (ANOVA) test, as required. P < 0.05 was considered statistically significant. Statistical analysis was performed using SPSS package, version 18.0 (SPSS, Inc., Chicago, IL)
Results
Over the 3-year study period, 350 patients with definite c-SLE diagnosis and 350 healthy controls were enrolled. The mean age of patients was (11.7±3.4) years and 332 (94.8%) were females with a disease duration of (mean, 4.7±1.6) years. Control subjects were well-matched for age, gender and ethnicity [mean age (11.2±2.8) years, 330 (94.2%) were females; all P > 0.05; Table 1].
Table 1.
Baseline Demographic, Clinical and Laboratory Data of Patients with cSLE and Control Group
| Patients n=350 (%) | Controls n=350 (%) | P | |
|---|---|---|---|
| Age(mean ± SD years) | 11.7±3.4 | 11.2±2.8 | 0.403a |
| Gender(male/female) | 18/332 | 20/330 | 0.186b |
| Age at diagnosis | 9.6±2.8 | NA | |
| Disease duration (years) | 4.7±1.6 | – | |
| Malar rash, n(%) | 151 (43) | – | |
| Photosensitivity, n(%) | 122 (35) | – | |
| Oral ulcers, n(%) | 105 (30) | – | |
| Discoid rash, n(%) | 56 (16) | – | |
| Alopecia, n(%) | 91 (26) | – | |
| Arthritis, n(%) | 133 (38) | – | |
| Serositis, n(%) | 77 (22) | – | |
| Neuropsychiatric disorders, n(%) | 38 (11) | – | |
| Hemolytic anemia | 189 (54) | – | |
| Thrombocytopenia | 108 (31) | – | |
| Leukopenia | 63 (18) | ||
| Positive ANA, n (%) | 343 (98) | – | |
| Positive Anti-ds DNA, n(%) | 326 (93) | – | |
| Lupus nephritis, n(%) | 241 (69) | – | |
| LN histologic grading | |||
| I | 29 (12) | – | |
| II | 55 (23) | ||
| III | 89 (37) | – | |
| IV | 68 (28) | ||
| Medications | |||
| I. Prednisolone | 350 (100) | – | |
| II. HCQ | 290 (83) | ||
| III. Immunosuppressive therapyc | 336 (96) | – | |
| SLEDAI-2K score | 6.8±1.5 |
Notes: Values in parentheses are percentages or data are presented as mean ± SD. aStudent’s t-test. bChi-square test. cCyclophosphamide, Azathioprine, and Mycophenolate mofetil.
Abbreviations: cSLE, childhood-onset systemic lupus erythematosus; ANA, antinuclear antibody; Anti-ds DNA, anti-double-stranded DNA antibody; LN, lupus nephritis; SLEDAI-2K, systemic lupus erythematosus Disease Activity Index; HCQ, hydroxychloroquine.
Baseline demographics, laboratory data and clinical parameters for patients and control group are listed in Table 1. The most frequent clinical presentations of c-SLE were active lupus nephritis (69%), pallor (54%), and malar rash (43%). Seropositive Anti-ds DNA and ANA were reported in 93% and 98% of patients, respectively, Table 1. Among 241 patients with LN, ISN/RPS class III was the most frequent (37%) followed by class IV (28%); Table 1.
The PAI-1 4G/5G genotype distribution and allele frequency in patients and control group were conformed to the Hardy–Weinberg equilibrium and are summarized in Table 2. The PAI-1 4G/4G genotype at position – 675 was more represented in c-SLE patients, as compared to the control group (38% vs.23%, respectively). Patients who were homozygotes for the PAI-1 4G/4G had 2.7- fold increased susceptibility to c-SLE (OR =2.7; [95% CI: 1.47–2.9]; P < 0.001); Table 2. Besides, PAI-1 4G allele at the same position was significantly more frequent among studied patients compared to control children (60% vs 51%; OR: 1.45; [95% CI: 1.2–1.8]; P < 0.01); Table 2.
Table 2.
Distribution of PAI-1 (4G/5G) Genotypes, and Allele Frequency in Patients with c-SLE and Control Group
| Model | Genotype | Patient group | Control group | OR(95% CI) | P | ||
|---|---|---|---|---|---|---|---|
| n(350) | % | n(350) | % | ||||
| Co-dominant | 5G/5G | 63 | (18) | 74 | (21) | Referent | |
| 4G/5G | 154 | (44) | 196 | (56) | 0.92 (0.61-1.4) | 0.768 | |
| 4G/4G | 133 | (38) | 80 | (23) | 2.7 (1.47-2.9) | < 0.001 | |
| Alleles | 5G | 280 | (40) | 344 | (49) | Referent | |
| 4G | 420 | (60) | 356 | (51) | 1.45 (1.2-1.8) | < 0.01 | |
| Dominant | 5G/5G | 63 | (18) | 74 | (21) | Referent | |
| 4G/5G+4G/4G | 287 | (82) | 276 | (79) | 1.2 (0.83-1.81) | 0.295 | |
| Recessive | 5G/5G+4G/5G | 217 | (62) | 270 | (77) | Referent | |
| 4G/4G | 133 | (38) | 80 | (23) | 1.95 (1.23-3.09) | 0.002 | |
| Over-dominant | 5G/5G+4G/4G | 196 | (56) | 154 | (44) | Referent | |
| 4G/5G | 154 | (44) | 196 | (56) | 0.62 (0.84-1.23) | 0.354 | |
| Serum PAI-1 (ng/ml) | 47.8±6.9 | 18.6±5.7 | 0.003a | ||||
Notes: Values in parentheses are percentages or data are presented as mean ± SD. P value < 0.05 indicates a significant difference. Chi-square test. aStudent’s t-test.
Abbreviations: OR, odds ratio; CI, 95% confidence interval; PAI-1, plasminogen activator inhibitor-1; cSLE, childhood-onset systemic lupus erythematosus.
We found that patients carrying the PAI-1 4G/4G genotype or 4G allele were more likely to develop lupus nephritis (OR: 3.38; [95% CI: 1.9–5.9]; P <0.001, for the 4G/4G genotype and OR: 2.6; [95% CI: 1.85–3.67]; for the 4G allele; P < 0.01), Table 3. In addition, PAI‐1 4G homozygotes tend to have a more active disease as demonstrated by higher SLEDAI-2K scores (P =0.02); Table 4. However, our data did not support a significant association between the PAI-1 4G/5G polymorphism and other clinical presentations or laboratory parameters of SLE among studied patients (all P > 0.05; Table 4).
Table 3.
Association of the PAI-1 4G/5G Genotypes and Alleles with Lupus Nephritis in Patients with Childhood-Onset SLE
| Genotype | Lupus Nephritis | OR(95% CI) | P | ||
|---|---|---|---|---|---|
| Presence | Absence | ||||
| n(241) % | n(109) % | ||||
| PAI-1 (−675) | 5G/5G | 28 (12) | 35 (32) | Referent | |
| 4G/5G | 102 (42) | 52 (48) | 0.80 (0.5–1.3) | 0.347 | |
| 4G/4G | 111 (46) | 22 (20) | 3.38 (1.9–5.9) | <0.001 | |
| Alleles | 5G | 158 (33) | 122 (56) | Referent | |
| 4G | 324 (67) | 96 (44) | 2.6 (1.85–3.67) | <0.01 | |
| Serum PAI-1 (ng/mL) | 68.5±11.4 | 43.6±9.2 | 0.02a | ||
Notes: Values in parentheses are percentages or data are presented as mean ± SD. P value < 0.05 indicates a significant difference. Chi-square test. aStudent’s t-test.
Abbreviations: SLE, systemic lupus erythematosus; PAI-1, plasminogen activator inhibitor-1; OR, odds ratio; CI, 95% confidence interval.
Table 4.
Association of the PAI-1 (4G/5G) Genotypes with Clinical, and Laboratory Features in Patients with Childhood-Onset SLE
| PAI-1 (−675) Genotype | 5G/5G (n= 63) |
4G/5G (n= 154) |
4G/4G (n =133) |
P |
|---|---|---|---|---|
| n (%) | n (%) | n (%) | ||
| Age at diagnosis(years) | 11.4±2.6 | 11±1.8 | 10.8±2.5 | 0.375a |
| Malar rash | 32 (50.7) | 83 (53.8) | 74 (55.6) | 0.164 |
| Photosensitivity | 21 (33) | 54 (35) | 47 (35) | 0.092 |
| Discoid rash | 10 (15.8) | 24 (15.5) | 22 (16.5) | 0.381 |
| Alopecia | 16 (25) | 39 (25) | 36 (27) | 0.642 |
| Arthritis | 33 (52) | 78 (51) | 68 (51) | 0.432 |
| Oral ulcers | 19 (30) | 43 (28) | 43 (32) | 0.706 |
| Serositis | 14 (22) | 32 (20.7) | 31 (23) | 0.529 |
| Neuropsychiatric disorders | 7 (11) | 16 (10) | 15 (11) | 0.312 |
| Hematologic disorder | 47 (74.6) | 113 (73) | 99 (74) | 0.824 |
| Immunologic disorder | 61 (97) | 146 (95) | 129 (97) | 0.127 |
| SLEDAI-2K | 4.8±1.3 | 5.3±0.8 | 11.6±2.4† | 0.02a |
Notes: Values in parentheses are percentages or data are presented as mean ± SD. P value < 0.05 indicates a significant difference. Chi-square test. aANOVA test. †Significant difference between each three genotypes group.
Abbreviations: SLE, systemic lupus erythematosus; PAI-1, plasminogen activator inhibitor-1; ANA, antinuclear antibody; SLEDAI-2K, systemic lupus erythematosus Disease Activity Index.
Of note, c-SLE patients had significantly higher mean PAI-1 serum concentrations than did control group (47.8±6.9 ng/mL vs 18.6±5.7 ng/mL, respectively; P =0.003); Table 2, Figure 2.
Figure 2.
PAI-1 serum levels in SLE patients and control group.
Moreover, patients with LN did have significantly higher PAI-1 serum levels compared to those without LN (mean; 68.5±11.4 ng/mL vs 43.6±9.2 ng/mL, respectively; P = 0.02); Table 3.
Patients carrying the PAI-1 4G/4G genotype had significantly higher PAI-1 serum concentrations (mean; 86.6±22.7 ng/mL) as compared to intermediate levels for those with a 4G/5G genotype (mean; 48.3±16.5 ng/mL) and the lowest for the 5G/5G genotype (mean; 34.7±11.4 ng/mL), respectively; P = 0.004; Table 5. The mean PAI-1 serum level for the 4G allele was (74.8±18.9 ng/mL) vs (32.5±14.6 ng/mL; P = 0.01) for the 5G allele at the same position; Table 5.
Table 5.
Comparison of PAI-1 Serum Levels in Patients with Childhood-Onset SLE and Control Group with Various PAI-1 (4G/5G) Genotypes and Alleles
| PAI-1 (4G/5G) Genotype | Serum PAI-1 (ng/mL) | |||
|---|---|---|---|---|
| SLE Patients | P | Control Group | P | |
| PAI-1 (−675) | ||||
| 5G/5G | 34.7±11.4 | 16.8±5.7 | ||
| 4G/5G | 48.3±16.5 | 21.5±4.9 | ||
| 4G/4G | 86.6±22.7† | 0.004a | 35.4±8† | 0.01a |
| Allele | ||||
| 5G | 32.5±14.6 | 18.2±4.4 | ||
| 4G | 74.8±18.9 | 0.01b | 34.6±7.6 | 0.03b |
Notes: Data are presented as mean±SD. P value < 0.05 indicates a significant difference. †Significant difference between each three genotypes group. aANOVA test. bStudent’s t-test.
Abbreviations: PAI-1, plasminogen activator inhibitor-1; SLE, systemic lupus erythematosus.
Interestingly, healthy controls with the 4G/4G genotype were also found to have the highest PAI-1 serum levels (mean; 35.4±8 ng/mL), compared with those carrying the 4G/5G or 5G/5G genotype (mean; 21.5±4.9 and 16.8±5.7 ng/mL, respectively; P = 0.01); Table 5.
Discussion
Recent studies have linked the PAI-1 4G/5G polymorphism with a variety of human diseases such as meningococcal disease, sepsis, obesity, metabolic syndrome, cancer, primary nephrotic syndrome, and autoimmune disorders.11,22–26
To the best of the authors’ knowledge, ours is the first such study to investigate PAI-1 4G/5G polymorphism in Caucasian children and adolescents with SLE.
In this study, the PAI-1 4G allele and 4G/4G homozygous genotype at position – 675 were significantly more represented among SLE patients compared to the control group. In addition, we found that the PAI-1 4G allele and 4G/4G genotype at the same position may constitute risk factors for developing lupus [OR: 1.45 and 2.7, respectively] and lupus nephritis [OR: 2.6 and 3.38, respectively]. Moreover, we observed that the PAI-1 4G/4G genotype was associated with a more active disease as demonstrated by higher SLEDAI-2K score among studied patients. However, no significant association was evident between the PAI-1 4G/5G SNP and other clinical phenotypes or laboratory profile of SLE. To date, only few studies in the literature investigated the PAI-1 4G/5G polymorphism in SLE adult patients with conflicting results.
Anaya-Macias et al reported that the PAI-1 4G/4G genotype and 4G allele were identified as genetic markers for increased susceptibility to SLE in Mexican-Mestizo population.27 Eldeeb et al studied the PAI-1 4G/5G SNP on genomic DNA of 170 Egyptian patients with adult-onset SLE. The authors concluded that the PAI-1 4G homozygote was identified as a risk factor for developing proliferative LN.28 Wang et al reported that the PAI-1 4G/4G genotype could predict higher nephritis activity, and more extensive necrotizing lesions without genetic association to the development of SLE or LN in Chinese population.29 Bates et al detected the presence of anti-PAI-1 autoantibodies in a cohort of British SLE patients, correlated with both anti-double stranded DNA (ds DNA) autoantibody titer and the disease activity index scores.30
In contrast, previous studies did not identify any association between the PAI-1 4G/5G polymorphism and lupus susceptibility or its clinical expression in Russian, Bulgarian, Spanish, and Argentinean SLE cohorts with adult-onset disease.31–34 Similarly, a recent meta-analysis of nine studies demonstrated that the PAI-1 4G/5G polymorphism did not increase the risk of SLE or LN among Asian and European populations, although they reported significant up-regulation of circulating PAI-1 activity in multi-ethnic cohorts with adult-onset SLE.11
Ethnic background or gene-environmental interplay as well as differences in cohort size and studied age group may partly explain these discrepancies between our study and previously published data.
PAI-1 is an acute phase protein regulated by inflammatory cytokines, chemokine, growth factors, hormones, and bacterial endotoxins.8 PAI-1 is also unique among serpins superfamily as it is expressed by various cell types and has multiple molecular interactions.8,9 PAI-1 resides at a key site in the fibrinolysis pathway and its overexpression compromises fibrin clearance, thus promoting microthrombosis.6,7 PAI-1 is also up-regulated by pro-inflammatory cytokines such as interleukin-6, IL-1β, tumor necrosis factor-α, and tissue growth factor-β. Moreover, PAI-1 enhances inflammation and cytokines release in a cyclic manner and may be a biological parameter for an ongoing inflammatory process.35 The PAI-1 activity has also been shown to inhibit apoptosis in different cell lines which may contribute to tolerance breakdown and autoimmunity.36 Therefore, this molecule exhibits a unique role in many pathophysiologic processes such as thrombosis, inflammation and apoptosis which may represent different scenarios for PAI-1 contribution in SLE pathogenesis.
In an attempt to explain our findings, we investigated PAI-1 serum concentrations in patients with SLE which were significantly higher than did healthy control group. Moreover, patients with LN had increased mean serum PAI-1 concentrations as compared to those without nephritis. These findings confirm and extend the results of previous studies in Turkish, Indian and Brazilian cohorts with adult-onset SLE.37–39
In this study, we speculate that the PAI-1 4G/5G SNP may alter PAI-1 expression level as we observed that the 5G to 4G switch at −675 position was associated with increased PAI-1 serum concentrations among studied subjects and the PAI-1 5G/5G, 5G/4G, and 4G/4G genotypes were associated with low, intermediate, and high PAI-1 serum levels, respectively. Consistent with these findings, previous studies have explained that the deletion of the PAI 4G allele (only 4 guanine bases) has been associated with the inability to bind a repressor protein that decreases binding of the transcriptional activator, resulting in up to 6 times more PAI-1 mRNA expression and increased circulating PAI-1 levels.15,40
The PAI‐1/t‐PA dynamic balance is involved in fibrin deposition in glomeruli, and extracellular matrix turnover. PAI‐1 mRNA levels were found to be strongly up-regulated in murine LN models, meanwhile treatment with anti-PAI-1 monoclonal antibody provided protection against the pathological changes in its kidneys.12,41 Elevated PAI‐1 circulating levels have been reported to be one of the major reasons for impaired fibrinolysis and a hypercoagulability state that promote thrombosis in patients with SLE.42 Gong et al reported that the PAI‐1 4G/4G genotype predispose to glomerular microthrombosis in LN patients.6,7 Consistent with our view, the authors explained that PAI‐1 4G homozygotes tend to have higher PAI‐1 serum concentrations; therefore, their fibrinolytic activity was blunted. Glomerular microthrombosis could incite and aggravate renal inflammation and perpetuate the lupus‐induced kidney destruction, thus worsen renal dysfunction.7 It remains unclear if the PAI-1 4G allele enhances the phenotypic expression of thrombophilia in patients with cSLE. However, a sole causal relationship between the PAI-1 4G/5G SNP and the risk of glomerular microthrombosis in pediatric patients with LN should be investigated further.
Because PAI-1 is considered to be a risk factor for many human diseases, extensive research are underway to develop selective PAI-1 inhibitors either by the use of monoclonal antibodies or antisense oligonucleotides that can inhibit PAI-1 synthesis, no such inhibitors are clinically applied yet.
Combining multi-ethnic population genomics with clinical profiling, and bioinformatics will provide new insights into biomarker development and novel targets for cSLE therapy.
Since PAI-1 serum level has a diurnal variation, a cross-sectional measurement may constitute a possible limitation in this study. All included patients had SLEDAI score > 4; indicating active disease at the time of inclusion, so the effect of inflammation on PAI-1 serum levels; as one of acute phase reactants, could not be excluded. Also, we have studied a single PAI-1 gene polymorphism which may exhibit an epistatic interaction or linkage disequilibrium with PAI-1 genomic loci or complex haplotypes directly affecting PAI-1 activity. Accordingly, genome-wide association studies are awaited across different ethnic populations to validate our findings.
Conclusion
The PAI-1 4G/5G polymorphism may confer susceptibility to childhood-onset SLE and development of lupus nephritis among Egyptian children and adolescents. Moreover, the PAI-1 4G/4G genotype and 4G allele were associated with higher PAI-1 serum levels and higher disease activity scores.
Finally, PAI-1 may be a promising biomarker and potential molecular target for novel therapeutics in autoimmune and inflammatory disorders such as childhood-onset SLE.
Abbreviations
SLE, systemic lupus erythematosus; cSLE, childhood-onset systemic lupus erythematosus; LN, lupus nephritis; ESRD, end stage renal disease; PAI-1, plasminogen activator inhibitor-1; t-PA, tissue type-plasminogen activator; U-PA, urokinase type-plasminogen activator; ELISA, enzyme-linked immunosorbent assay; SNPs, single nucleotide polymorphisms; SLEDAI, Disease Activity Index; PCR-RFLP, polymerase chain reaction-restriction fragment length polymorphism; OR, odds ratio; CI, confidence interval.
Author Contributions
All authors made a significant contribution to the work reported, whether that is in the conception, study design, execution, acquisition of data, analysis and interpretation, or in all these areas; took part in drafting, revising or critically reviewing the article; gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work.
Disclosure
The authors report no conflict of interest in this work.
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