Figure 4.
Concordant and Discordant Gene Changes with Micro- and Hypergravity
(A) Overlap of differentially expressed hypergravity genes (15 × g) and reproducibly differentially expressed microgravity genes (|LFC| > 0). Green shading denotes strength of Fisher's exact odds ratio, with corresponding significance and number of overlapping genes provided within each corresponding box.
(B) Non-redundant, enriched Gene Ontology Biological Process terms for each possible hypergravity versus microgravity overlap permutation. Number of genes enriched in a given term are provided within associated boxes of the heatmap. Darker shading denotes greater significance.
(C): Predicted transcription factors of each overlapping gene set.
(D): Quantity (in boxes) and significance (green shading scale) of enriched protein interactions among genes of each possible overlap, across range of interaction “confidence” cutoffs.
(E) Protein interactions (confidence >0.15) for genes commonly upregulated with hypergravity/microgravity, as well as genes upregulated with hypergravity but downregulated by microgravity. Larger nodes depict “hub” proteins (hub score >0.8), with top-ranked hub protein in each case identified by a green node border.