Figure 8.

Anti-CXCR2 mAbs treatment reduces leukocytes infiltration to the inflamed ankle and popliteal draining lymph nodes. C57BL/6 WT or hCXCR2 -KI mice were injected i.p. 200uL of K/BXN serum on day 0 and 1. When mice were exhibiting symptoms of arthritis and the cumulative clinical score reached 4 (on day 4), mice split into two groups and injected i.p. SAMX2-FcKO, HAHX2-FcKO, or isotype control mAb at a concentration of 20 mg/kg body weight, followed by 5 mg/kg every other day for 1 week. Flow cytometric analysis demonstrates the effect of SAMX2-FcKO and HAHX2-FcKO mAb treatment on the innate effector cells compare to a control group that did not receive K/BXN serum or antibody treatment (normal control): (a) C57BL/6 WT mice ankle and (b) hCXCR2-KI mice ankle, (c) C57BL/6 WT mice draining lymph nodes and (d) hCXCR2-KI mice draining lymph nodes, at the endpoint. Each data point represents an individual mouse, and n = 6 in each group. All data represented as mean ± SEM. Each graph is representative of three independent experiments. Statistics were calculated using one-way analysis of variance with Tukey posttest. *P < .05, **P < .01, ***P < .005, ****P < .0001