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. 2020 Dec 14;9:e55730. doi: 10.7554/eLife.55730

Figure 1. Cardiac endothelial cells express myofibrillar genes normally associated with cardiomyocytes.

Figure 1.

(A) RNASeq of Translating Ribosome Affinity Purification (TRAP) endothelial RNA freshly isolated from hearts of Cdh5-NuTRAP animals. Expression (Log2CPM) of immunoprecipitated endothelial RNA (IP) versus non-endothelial RNA from the remaining fraction after IP (supernatant). Shown in the blue box are the top 300 expressed genes, with CMF genes highlighted in red. Kendall-Tau rank analyses performed on these top 300 expressed genes. (B) Relative expression of selected genes from freshly isolated tissue-specific adult endothelial cell TRAP, collected from (Rpl22fl/fl, Tek2-Cre+)/animals (Cleuren, et al, GSE138630) vs total tissue TRAP RNA (Rpl22fl/fl, EIIa-Cre+/0). (C) Kendall-Tau analysis of TRAP vs total tissue of heart tissue from Cleuren, et al. Analyses performed on the highest 300 expressed genes in the total heart tissue (D) Multidimensional scaling (MDS) clustering of the top 1000 variably expressed genes in the tissues analyzed in (B). (E) Relative expression of the indicated genes in tissue-specific adult murine endothelial cells in data sets from Coppiello et al., 2015 (GSE48209) and Nolan et al., 2013 (GSE47067) (F) Relative expression of tissue-specific fetal human endothelial RNASeq from Marcu et al., 2018 (GSE114607).