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. 2020 Dec 15;9:e63665. doi: 10.7554/eLife.63665

Figure 1. In adipocytes p53 can be up-regulated by nutrient starvation, but without canonical pathway activation.

Figure 1.

(A) Three groups of WT C57 mice were either not treated (Control), fasted for 24 hr (Fasting), or followed by refeeding for 24 hr (Refeeding). Primary adipocytes isolated from epididymal fat tissue were subjected to western blot using indicated antibodies. (B–C) p53 protein levels in isolated primary adipocytes from mice fed a high-fat diet (HFD) for 3 days (B) or 12 weeks (C) were examined by western blots. The quantification of relative p53 expression levels was normalized by tubulin and shown in bar graphs. (D–E) Gene expressions of p53 canonical pathway were measured by RT-PCR in samples from A-C. (F) The protein expression levels of p53 and actin were measured from 3T3-L1 adipocytes cultured in three media conditions: normal culture (DMEM with 10% FBS), fasting (PBS with 1% BSA and ISO, 10 mM for 6 hr), and refeeding (normal plus insulin for 6 hr after fasting). (G) Gene expressions of p53 canonical pathway were measured by RT-PCR in samples from F.

Figure 1—source data 1. An Excel sheet with numerical quantification data.