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. Author manuscript; available in PMC: 2020 Dec 24.
Published in final edited form as: Biochemistry. 2020 Feb 4;59(6):790–801. doi: 10.1021/acs.biochem.9b01006

Figure 7. Amino acid substitutions tune the BirA allosteric response.

Figure 7.

A. Amino acid substitutions in BirA yield a broad range of holoBirA dimerization free energies. The bracketed line above the bars indicates variants with substitutions that dimerize with free energies within ±1 kcal/mol of holoBirAwt and asterisks signify residues that contribute directly to the dimer interface. B. C-alpha carbons of amino acid positions in A shown on the holoBirA monomer with small spheres signifying modest (within ±1 kcal.mol) and large spheres indicating larger (>±1 kcal/mol) effects on the dimerization free energy. Color code: Loops, Orange:116-124; Red:140-146; Purple:170-176; Green:193-199; Cyan:211-222; Blue:280-283; Yellow:310-313; Gray: Protein Core. The model was created in Pymol with pdb file 2EWN as input.