Extended Data Fig. 9. Characterization of a translocation involving wheat chromosomes 5B and 7B.

a, Cytogenetic karyotypes of Forno (left) and Arina (right), the parents of ArinaLrFor. Note that the large recombinant chromosome 7B is represented by a distinct peak. b, Sequence of the translocation breakpoint on chromosome 7B of ArinaLrFor. Note that the exact breakpoint lies in a sequence gap (stretch of Ns). The bp positions are indicated at the left. Forward PCR primers are shown in red and reverse primers in blue. The overlap of the two reverse primers is shown in purple. The outer primer pair was used for PCR, while the inner pair was used for a nested PCR. c, PCR amplification of the fragment spanning the translocation breakpoint. The nested PCR yielded a ~5 kb fragment that spanned the translocation breakpoint and its identity was confirmed by sequencing. Both PCR and nested PCR were performed in duplicate; both replicates of the nested PCR were sequenced using the Sanger method. For gel source data, see Supplementary Fig. 1. d, Mapping of Illumina reads from the cultivars Arina and Forno on to the pseudomolecules of ArinaLrFor. Sequence derived from Forno is shown in blue, while sequenced derived from Arina is in red. Note that chromosomes 5B and 7B are derived from both parents, indicating that these parental chromosomes can recombine freely, despite the presence of a large 5B/7B translocation in Arina.