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. Author manuscript; available in PMC: 2021 Mar 3.
Published in final edited form as: Biochemistry. 2020 Feb 24;59(8):921–932. doi: 10.1021/acs.biochem.9b00993

Figure 3. Effective concentration (EC50) of E2 stimulation of OTUB1 activity.

Figure 3.

Cleavage of FRET-K48 diUbiquitin (400 nM) by OTUB1 (50 nM) was assayed with increasing concentrations of E2 enzymes (1 pM to ~50 μM). Reactions were assayed in triplicate.