Figure 3.

Functional characterization of best hits in IP1 production inhibition assay. Inhibition of LTD4- (a) or LTC4- (b) induced IP1 production by selected compounds. Experiments were performed in HEK293 cells transiently expressing the wild-type CysLT1R or CysLT2R. Curves correspond to normalized data; values from non-stimulated cells were set as 0% IP1 production and those from cells stimulated with either LTD4 or LTC4 were set as 100% IP1 production. Data represent mean ± SEM of three independent experiments, tested in quadruplicate. NS = non-stimulated.