Table 2.
Recovery percentages (% ± SD) for atropine and scopolamine in standard solutions (Ss) and buckwheat (Bu-F1O) and sorghum (So-FO) flours extracts using sulfonic acid-functionalized SBA-15 and commercial materials as SCX-SPE sorbents *.
| Solvent | M-SBA-15-SO3− SCX-SPE Sorbent | MFE-PAK® SCX-SPE Sorbent | ||||||
|---|---|---|---|---|---|---|---|---|
| Atropine | Scopolamine | Atropine | Scopolamine | |||||
| Ss | Bu-F1O | So-FO | Ss | Bu-F1O | So-FO | Ss | Ss | |
| ACN/H2O, 0.5% HCOOH (3:5, v/v) | 74 ± 2 | 46± 22 | 10 ± 1 | 81 ± 4 | 5 ± 1 | 8 ± 1 | - | - |
| ACN/H2O, 1% HCOOH (3:5, v/v) | 78 ± 1 | 7 ± 3 | 7 ± 2 | 80 ± 5 | 4 ± 2 | 7 ± 3 | - | - |
| MeOH/H2O, 0.5% HAc (2:1, v/v) | 86 ± 3 | 16 ± 3 | 15 ± 2 | 105 ± 10 | 12 ± 1 | 16 ± 0 | 65 ± 3 | 65 ± 2 |
| H2O, 1.1% HCl (pH 1.0) | 90 ± 7 | 43 ± 10 | 66 ± 3 | 103 ± 1 | 25 ± 7 | 48± 3 | 36 ± 4 | 15 ± 2 |
* SPE experimental conditions - Sorbent amount: 50 mg. Loading: 8 mL of 0.1 mg/L TAs standard solution or sample extracts from flours spiked with 0.1 mL of a 1 mg/L TAs standard solution. Elution: 3 mL of MeOH and 2 × 3 mL MeOH + 10% of ammonia solution (pH 11.8). Conditioning (5 mL) and washing (3 mL) of the same mixture used for the loading step.