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. 2020 Dec 12;12(12):1428. doi: 10.3390/v12121428

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cloning of the CS sequence into reporter constructs transfers cleavage activity. (A) Schematic representation of constructs expressing mCherry and Gaussia luciferase (GL) separated by the P2A or CS sequences. The amino acid sequences of the P2A and CS are shown. (B) Western blots showing detection of mCherry (anti-RFP antibody [5F8], shown in green) in cell lysate (left blot) and supernatant (right blot) of HEK-293T cells transfected with mCh–CS–GL and mCh-P2A-GL. Black arrowhead indicates position of cleavage product, white arrowhead indicates precursor protein. One of three independent experiments is shown.