Table 5.

Biomolecules biosynthesized by greenhouse plants and in vitro types of calli of Phyllanthus amarus under different conditions and identified by GC-MS.

Rt (min)	Formula	Molar Weight	Biomolecule	Greenhouse Plants	Callus				References
					BA + Light (Relative Content %)			2,4-D + Dark
		(g·mol−1)		(Leaves + Stem)	Control	Salicylic Acid	Chitosan	Salicylic Acid
10.86	C15H24	204	alpha-muurolene		1.33	2.23			NIST-11
			ni				0.61
		298	ni					13.90
		300	ni		3.97	2.49		14.69
		314	ni					0.81
		316	ni					1.50
		386	ni	3.15%
		392	ni					0.50
		394	ni					0.59
16.40	C23H30O6	402	5-demethoxy-niranthin	23.34%					Library CPQBA
16.91	C24H34O6	418	Phyllanthin	30.03%					Library CPQBA
17.02	C24H32O6	416	Phyltetralin	6.19%					Library CPQBA
17.34	C23H28O6	400	5-demethoxy-nirtetralin	10.01%					Library CPQBA
18.26	C24H30O7	430	Nirtetralin	3.07%					Library CPQBA
19.85	C24H32O7	432	Niranthin	18.92%					Library CPQBA
24.96	C29H48O	412	Stigmasterol		5.22	8.14	5.45	8.02	NIST-11
25.04	C30H50	410	Squalene					0.73	NIST-11
26.86	C29H50O	414	γ-Sitosterol		2.56	3.35	5.14	6.72	NIST-11
28.42	C30H50O	426	β-Amyrin		2.58	2.57			NIST-11
		422	ni		9.93	8.95	7.90	29.10
			ni
			ni		4.74	4.57	2.60
			ni			2.34
			ni			6.2
32.67	C30H48O2	440	Betulin aldehyde or betulone		62.5	60.92	75.98	4.69	NIST-11
			ni		4.29		2.32
			ni		2.88
		Total Molecules		7	10	10	7	11

ni, not identified molecules presented in calli and detected by GC-MS; NIST-11, National Institute of Standards and Technology Library; CPQBA, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas.