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. 2020 Dec 10;25(24):5845. doi: 10.3390/molecules25245845

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Treatment with pyranoxanthone 2 (PX2) triggers the spindle assembly checkpoint. Immunofluorescence staining using antibodies against (a) Mad2 (green), (b) BubR1 (green), CREST (red) and DAPI (blue) in untreated and PX2-treated cells, as indicated. In untreated cells, Mad2 and BubR1 is not located in kinetochores, consistent with their normal localization pattern. In PX2-treated cells (bottom panel), these proteins are present in all mitotic cells, indicating spindle assembly checkpoint activation. Bar, 5 µm.