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. 2020 Dec 19;12(12):1468. doi: 10.3390/v12121468

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Phylogenetic analysis targeting the VP1/P2B junction of hepatitis A virus (HAV) strains detected in samples from all five wastewater treatment plants (WWTPs)—the tree highlights strains from the “SAn Minor cluster”. For better visualisation, branches are coloured red and brown to represent virus lineages from WWTP4 and 5, respectively. HAV strains detected in samples from WWTP2 (green) and WWTP5 (brown) are highlighted. Viruses detected after viability treatment are indicated with ■ (sewage) and ▲ (treated discharge). Previously characterised HAV strains from clinical sources in Gauteng (●) are indicated. The neighbour-joining tree was constructed using the Kimura two-parameter model. Bootstrap values greater or equal to 70% are shown at the nodes.