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. 2020 Dec 18;8(12):628. doi: 10.3390/biomedicines8120628

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Gene Expression of innate immune receptors after MgA treatment of promastigote-exposed spheroids. Samples of 3D-hepatocytes treated with MgA were collected to evaluate NOD1 (A), NOD2 (B), TLR2 (C), TLR4 (D), and TLR9 (E) mRNA accumulation at 1.5, 5, and 24 h of treatment. Untreated hepatocyte aggregates exposed to parasites for 72 h (LiPexp) also were evaluated. Results of 10 dogs performed in triplicate are represented by Tukey graphs. Black dots are indicative of outlier values. The non-parametric Wilcoxon test was used for statistical comparisons, and * indicates statistically significant differences (p < 0.05).