Table 1.
Organic content from thermogravimetric analysis of MSNs and functionalized MSNs materials. Concentration of non-anchored protein from Bradford assay of protein functionalized MSNs.
| Material | Org. Content (wt%) | [ProteinMtb] Nominal (μg/mL) 2 | [Non-Anchored ProteinMtb] (μg/mL) | % Anchored ProteinMtb |
|---|---|---|---|---|
| MSNs | 3.9 | --- | --- | --- |
| MSNs-COOHext-CTAB | 34.6 1 | --- | --- | --- |
| MSNs-COOHext | 5.4 1 | --- | --- | --- |
| MSNs-Ag85B | 8.1 1 | 91.9 | 0.64 | 99% |
| MSNs-LprG | 8.7 1 | 67.47 | 0.56 | 99% |
| MSNs-LprA | 8.6 1 | 71.96 | 3.89 | 95% |
1 Organic content (wt%) was determined from the TGA weight losses, excluding the weight loss due to the desorption of water (up to 125 °C) and further corrected by the weight loss of the remaining alkoxysilanes after the sol-gel reaction (surfactant extracted unmodified MSNs). 2 The nominal concentration of each protein was calculated considering the amount of proteins utilized in the volume of reaction.