Extended Data Figure 7. Reelin is efficiently deleted in Reln^ΔLEC/ΔLEC cardiac-associated lymphatics.

a, Immunostaining of E17.5 control and Reln^ΔLEC/ΔLEC heart sections with antibodies against Reelin and Lyve1 confirms that Reelin is deleted from cardiac lymphatics in Reln^ΔLEC/ΔLEC hearts (TAM injected at E13.5 and E14.5). N = 3 embryos/genotype from 2 separate litters. Control embryos are TAM treated Cre^- embryos and Cre⁺; Reln^+/+embryos. b, Co-immunostaining with antibodies against Vimentin, PECAM1, CD68, Six2 and Hnf4α, together with EdU labeling shows comparable proliferation of cardiac fibroblasts, blood endothelial cells and macrophages, and of nephron progenitors and hepatocytes between controls and E17.5 Reln^ΔLEC/ΔLEC hearts (TAM injected at E13.5 and E14.5). White arrows indicate EdU+ proliferating cells. Quantification of the proliferation for each of those cell types is shown on the right panels. N = 3 embryos/genotype from 2 separate litters. Control embryos are TAM treated Cre^- and Cre⁺;Reln^+/+ littermates. Data are presented as mean ± S.E.M. n.s. not significant difference by unpaired two-tailed Student’s t test. Scale bar, 25 μm.