Figure 4.

The Bmal1 protein level decreases dramatically in the brain of old turquoise killifish

(A and B) Protein levels, phosphorylation patterns (left), and quantified protein levels (right) of Bmal1 (A) and Clockb (B) under LD.

(C and D) (C) Bmal1 and Clockb protein levels (left) and their quantification (right) under DD. The protein levels were normalized to that of tubulin. Data are presented as the mean ± SD from three biological replicates. ∗p < 0.05 and ∗∗∗p < 0.001 after two-way ANOVA followed by the Sidak's multiple comparison test.

See also Figures S5 and S6 and Table S1.