REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal anti-Cox1 (clone D2G6)	Cell Signaling Technology	Cat# 9896S; RRID: AB_10860249
Rabbit monoclonal anti- Cox2 (clone D5H5)	Cell Signaling Technology	Cat# 12282S; RRID: AB_2571729
Control rat IgG2a, κ Isotype	Biolegend	Cat# 400501; RRID: AB_326523
Control mouse IgG2b, κ Isotype	Biolegend	Cat# 401201; RRID: AB_2744505)
anti-Gr1 (clone RB6-8C5)	Biolegend	Cat# 108414; RRID: AB_313379
anti-NK1.1 (clone PK136)	BioXCell	Cat# BE0036; RRID: AB_1107737
Ultra-LEAF Purified anti-Asialo-GM1 Antibody	Biolegend	Cat# 146002; RRID: AB_2562206
anti-CD4 (clone GK1.5)	BioXCell	Cat# BE0003-1; RRID: AB_1107636
anti-CD8alpha (clone YTS169.4)	BioXCell	Cat# BE0117; RRID: AB_10950145
anti-CD49b-APC (clone DX5)	BioLegend	Cat# 103516; RRID: AB_2566101)
anti-Ly6G- PE/Dazzle 594 (clone 1A8)	BioLegend	Cat# 127648; RRID: AB_2566319
anti CD4-Alexa700 (RM4-5)	BioLegend	Cat# 100536; RRID: AB_493701
anti-CD8a-PE (clone 53-6.7)	BioLegend	Cat# 100708; RRID: AB_312747
CD45-BV605 (Clone 30-F11)	eBioscience	Cat# 103155; RRID: AB_2650656
CD11b-BV785 (Clone M1/70)	BioLegend	Cat# 101243; RRID: AB_2561373
Ly6C-FITC (Clone AL- 21)	BD Bioscience	Cat# 553104; RRID: AB_394628
F4/80-PE-Cy7 (Clone CI: A3-1)	BioLegend	Cat# 123114; RRID: AB_893478
anti-MHCII I-A/I-E-Alexa700 (Clone M5/114.15.2)	BioLegend	Cat# 107622; RRID: AB_493727
anti-MHCII I-A/I-E- APC- eFluor780 (Clone M5/114.15.2)	Thermo Fisher Scientific	Cat# 47-5321-82; RRID: AB_1548783
anti-CD11c-PerCP/Cy5.5 (Clone N418)	BioLegend	Cat# 117328; RRID: AB_2129641
anti- CD103-APC (Clone 2E7)	BioLegend	Cat# 121414; RRID: AB_1227502
anti- CD103-PE (Clone 2E7)	BioLegend	Cat# 121406; RRID: AB_1133989
NK1.1-APC (Clone PK136)	BioLegend	Cat# 108710; RRID: AB_313397
NK1.1- PE (Clone PK136)	BioLegend	Cat# 108708; RRID: AB_313395
CD49b-APC (Clone DX5)	BioLegend	Cat# 108910; RRID: AB_313417
XCR1- Alexa647 (Clone ZET)	BioLegend	Cat# 148214; RRID: AB_2564369
XCR1-BV421 (Clone ZET)	BioLegend	Cat# 148216; RRID: AB_2565230
Siglec-H-BV711 (Clone E50-2440)	BD Bioscience	Cat# 740764; RRID: AB_2740427
IFNγ-eFluor450 (Clone XMG1.2)	Thermo Fisher Scientific	Cat# 48-7311-82; RRID: AB_1834366
CD44-APC-eFluor780 (Clone IM7)	Thermo Fisher Scientific	Cat# 47-0441-82; RRID: AB_1272244
CD3ε-Percp-Cy5.5 (Clone 145-2C11)	BioLegend	Cat# 100328; RRID: AB_893318
CD3ε-APC (Clone 145-2C11)	BioLegend	Cat# 100312; RRID: AB_312677
CD8a-PE (Clone 53-6.7)	BioLegend	Cat# 100708; RRID: AB_312747
CD8a-PE-Cy7 (Clone 53-6.7)	BioLegend	Cat# 100722; RRID: AB_312761
CD4-FITC (Clone RM4-5)	BioLegend	Cat# 100510; RRID: AB_312713
CD4-APC-eFluor780 (Clone GK1.5)	Thermo Fisher Scientific	Cat# 47-0041-82; RRID: AB_11218896
anti-CD16/32 (clone 2.4G2)	BioLegend	Cat# 101302; RRID: AB_312801
affinity purified anti-Ly6G (Clone 1A8)	BD Bioscience	Cat# 551459; RRID: AB_394206
affinity purified anti-NK1.1- biotynilated (Clone PK136)	BioLegend	Cat# 108704; RRID: AB_313391
CD19-eFluor450 (Clone eBio1D3)	Thermo Fisher Scientific	Cat# 48-0193-82; RRID: AB_2734905
Chemicals, Peptides, and Recombinant Proteins
Collagenase IV	Worthington Biochemical	Cat# LS004188
DNase I	Roche	Cat#11284932001
Aqua LIVE/Dead-405 nm staining	Thermo Fisher Scientific	Cat# L34957
Brefeldin A Solution (1,000X)	Biolegend	Cat# 420601
Monensin Solution (1,000X)	Biolegend	Cat# 420701
10 μm latex beads	Beckman Coulter	Cat# 6602796
OCT embedding medium	Thermo Fisher Scientific	Cat# 12678646
Recombinant FC IL-2	Thermo Fisher Scientific	Cat# 34-8021-85
CellTrace Calcein Green	Thermo Fisher Scientific	Cat# C34852
Calcein Red- Orange	Thermo Fisher Scientific	Cat# C34851
RLT lysis buffer	QIAGEN	Cat# 79216
PureZOL RNA isolation Reagent	BioRad	Cat# 7326890
Critical Commercial Assays
RNeasy RNA isolation Kit	QIAGEN	Cat# 74106
Direct-zol RNA MiniPrep Kit	Zymo Research	Cat# R2052
High Capacity cDNA archive Kit	Applied Biosystems	Cat# 4368813
MojoSort Mouse NK Cell Isolation Kit	Biolegned	Cat# 480050
Lexogen QuantSeq 3′-mRNaseq Library Prep Kit FWD	Lexogen	Cat# 015
Chromium Single Cell 3′ Reagent Kit v3	10X Genomics	PN- 1000075, PN-120262
Chromium Chip B	10X Genomics	PN-1000073
Kapa Library Quantification Kit for Illumina sequencing platforms	Kapa Biosystems	Cat# KK4873
Prostaglandin E2 Express ELISA Kit	Cyman Chemical	Cat# 500141
Deposited Data
Raw and processed data (bulk tumor RNaseq)	This paper	GEO: GSE139044
Raw and processed data (sorted TAM RNaseq)	This paper	GEO: GSE139045
Raw and processed data (single cell RNaseq)	This paper	GEO: GSE139046
Table of cluster biomarkers (scRNaseq)	This paper	Table S1
DEG analysis in Myeloid population (scRNaseq)	This paper	Table S2
Single sample GSEA (scRNaseq)	This paper	Table S3
Ingenuity Pathway Analysis (sorted TAM RNaseq)	This paper	Table S4
Signature gene list (human dataset analysis)	This paper	Table S5
Immune infiltrate in neutrophil-depleted tumor bearing mice (FACS analysis)	This paper	https://doi.org/10.17632/gngxk9mjkm.1
Experimental Models: Cell Lines
Melanoma C57BL/6 Braf +/LSL-V600E;Tyr::CreERT2 +/o;p16 INK4a−/− cell line	Dhomen et al., 2009	Ptgs+/+
Melanoma C57BL/6 Braf +/LSL-V600E;Tyr::CreERT2 +/o;p16 INK4a−/− cell line COX1/2-deficient	Zelenay et al., 2015	Ptgs−/−
CT26	Cancer Research UK Manchester Institute	N/A
MC38	Cancer Research UK Manchester Institute	N/A
4T1	Cancer Research UK Manchester Institute	N/A
CT26 COX2-deficient	Zelenay et al., 2015	N/A
MC38 COX2-deficient	This paper	N/A
4T1 COX1/2-deficient	Zelenay et al., 2015	N/A
Experimental Models: Organisms/Strains
Mouse: C57BL/6JOlaHsd	ENVIGO	Stock No: 057
Mouse: BALB/cOlaHsd	ENVIGO	Stock No: 162
Mouse: Rag1−/−	Cancer Research UK Manchester Institute	N/A
Mouse: Ifng−/−	Cancer Research UK Manchester Institute	N/A
Mouse: Tmem173−/−	Cancer Research UK Manchester Institute	N/A
Mouse: Cgas−/−	Cancer Research UK Manchester Institute	N/A
Mouse: Trif−/−	Cancer Research UK Manchester Institute	N/A
Mouse: Mavs−/−	Cancer Research UK Manchester Institute	N/A
Mouse: Myd88−/−	Cancer Research UK Manchester Institute	N/A
Mouse: Gzmb-Cre Ptger4floxed/floxed /Ptger2−/−	Cancer Research UK Manchester Institute	N/A
Mouse: Gzmb-Cre Ptger4floxed/floxed	Cancer Research UK Manchester Institute	N/A
Mouse: Ptger2−/−	Cancer Research UK Manchester Institute	N/A
Mouse: Tcrd−/−	Instituto de Medicina Molecular, Lisbon	N/A
Oligonucleotides
Ccl2-Taqman probe	Thermo Fisher Scientific	Mm00441242_m1
Ccl5-Taqman probe	Thermo Fisher Scientific	Mm01302427_m1
Cd8a-Taqman probe	Thermo Fisher Scientific	Mm01182107_g1
Ptgs2-Taqman probe	Thermo Fisher Scientific	Mm00478374_m1
Cxcl1 -Taqman probe	Thermo Fisher Scientific	Mm04207460_m1
Cxcl10-Taqman probe	Thermo Fisher Scientific	Mm00445235_m1
Cxcl2-Taqman probe	Thermo Fisher Scientific	Mm00436450_m1
Cxcl9-Taqman probe	Thermo Fisher Scientific	Mm00434946_m1
Eomes-Taqman probe	Thermo Fisher Scientific	Mm01351984_m1
Csf3-Taqman probe	Thermo Fisher Scientific	Mm00438334_m1
Gapdh-Taqman probe	Thermo Fisher Scientific	Mm99999915_g1
Gzmb -Taqman probe	Thermo Fisher Scientific	Mm00442837_m1
Hprt-Taqman probe	Thermo Fisher Scientific	Mm03024075_m1
Ifng-Taqman probe	Thermo Fisher Scientific	Mm01168134_m1
Il12a-Taqman probe	Thermo Fisher Scientific	Mm00434169_m1
Il12b-Taqman probe	Thermo Fisher Scientific	Mm01288989_m1
Il1a-Taqman probe	Thermo Fisher Scientific	Mm00439620_m1
Il1b-Taqman probe	Thermo Fisher Scientific	Mm00434228_m1
Il6-Taqman probe	Thermo Fisher Scientific	Mm00446190_m1
Prf1-Taqman probe	Thermo Fisher Scientific	Mm00812512_m1
Stat1-Taqman probe	Thermo Fisher Scientific	Mm01257286_m1
Tbx21-Taqman probe	Thermo Fisher Scientific	Mm00450960_m1
Vegfa-Taqman probe	Thermo Fisher Scientific	Mm00437306_m1
Software and Algorithms
ImageJ/Fiji software	https://imagej.nih.gov/ij/	version 2.0.0-rc14/1.49
Ingenuity Pathways Analysis software (IPA)	QIAGEN	2018 update
Cell Ranger Pipeline	10X Genomics	version 3.1
GraphPad Prism	GraphPad Software	version 8.2.0
FlowJo Software	TreeStar FlowJo LLC.	version 10.4.2
Cytobank Software	CytoBank, Inc.	N/A
Partek Flow software (Single cell Toolkit)	Partek	build version 8.0.19.0610
R software	R project	N/A
Other
Mouse Diet: RM3 expanded and irradiated	SDS	Code: 801185