TABLE 1.

Significant effects of neonatal gut metabolites on C. parvum growth

Rank order of fold changea	Metabolite name	Concn (mM)	Avg growth ratio (mean ± SD)b	P value
11	Docosahexaenoic acid	0.1	1.44 ± 0.29	<0.0001
18	Linolenic acid	0.5	1.40 ± 0.02	<0.0001
13	Linoleic acid	0.5	1.33 ± 0.07	<0.0001
35	Dihydroxyacetone	0.5	1.22 ± 0.05	<0.0001
42	Behenic acid	0.02	1.22 ± 0.08	0.0003
31	Hexadecane	0.5	1.20 ± 0.07	0.0002
41	Benzylalcohol	0.5	1.19 ± 0.22	0.0006
34	N-acetyl-d-mannosamine	0.5	1.19 ± 0.10	0.0006
32	Pentadecanoic acid	0.1	1.18 ± 0.11	0.0009
23	Glucose-6-phosphate	0.5	1.18 ± 0.35	0.0022
17	Diphosphoric acid	0.5	1.16 ± 0.24	0.0089
20	Nicotinamide	0.5	1.15 ± 0.15	0.0150
29	Maltose	0.5	1.14 ± 0.12	0.0310
27	UDP-N-acetylglucosamine	0.5	1.14 ± 0.22	0.0390
15	Oxamic acid	0.5	1.13 ± 0.07	0.0488
10	1-Monostearin	0.5	0.78 ± 0.13	<0.0001
2	1-Monopalmitin	0.1	0.77 ± 0.21	<0.0001
19	Palmitic acid	0.1	0.77 ± 0.07	<0.0001
5	Myristic acid	0.5	0.68 ± 0.16	<0.0001
6	Lauric acid	0.5	0.61 ± 0.08	<0.0001
1	Monomyristin	0.5	0.59 ± 0.08	<0.0001
14	Capric acid	0.5	0.32 ± 0.07	<0.0001

^aRank order of metabolites by fold change in abundance from week 1 to week 3 as shown in Table S1.

^bRatio of C. parvum growth relative to the DMSO control averaged across three independent experiments, with two to three technical replicates per experiment, as shown in Fig. 3. Two way ANOVA with Dunnett's test for multiple comparisons.