Figure 4.

HES1 and HES4 repress NK cell development. (A) Flow cytometric analysis of control, ICN1-, HES1- and HES4-transduced CB CD34⁺Lin^– precursors cultured on the MS-5 feeder (Notch-OFF) for 3 weeks in the presence of the NK-lineage specific cytokines IL-7, SCF, FLT3L and IL-15, showing the development of CD56+CD7- and CD56⁺CD7⁺ NK cells. Plots shown are representative for six independent experiments. (B-C) Absolute numbers of CD56⁺CD7^– (B) and CD56⁺CD7⁺ (C) NK cells generated in corresponding cultures shown in (A). Data shows the average of six independent experiments. Error bars indicate the standard error of the mean (SEM). *P<0.05 (non-parametric paired Wilcoxon test); ns: not significant.