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. 2021 Jan 1;11(4):1655–1671. doi: 10.7150/thno.52710

Figure 7.

Figure 7

FUS+MB BBB treatments cause neutrophil recruitment. Polymorphonuclear neutrophils were observed in FUS+MB treated cortical tissue. (A, B) Brain sections were stained for neutrophils (MPO, magenta), astrocytes (GFAP, green), and nuclei (DAPI, cyan). Numerous MPO+ neutrophils were present in the dura (*), in both sonicated and control animals, likely due to the cranial window procedure. (C-E) Neutrophils were found within and outside of blood vessels. (C) A blood vessel is outlined by GFAP+ (green) astroglial processes. (D,E) Neutrophils were identified as MPO+ (magenta) cells containing multi-lobed nuclei (counter-stained with DAPI, cyan). (F) Neutrophils were more numerous and located deeper in the cortex in FUS+MB treated animals (blue), compared to control animals (orange). Scale bars: (A,B) 50 µm, (C-E) 10 µm. GFAP: glial fibrillary acidic protein, MPO: myeloperoxidase.