Table 3.
Summary of differential expression levels across different sample libraries
| Expression | log2 Fold Change (FC) | FDR p value | Number of transcripts | ||
|---|---|---|---|---|---|
| IMFvsFF | MFvsFF | IMFvsMF | |||
| Up-regulated | ≥ 1 | ≤ 0.001 | 3003 | 4259 | 219 |
| ≥ 1 | 0.001 < P ≤ 0.05 | 5082 | 6302 | 1025 | |
| ≥ 1 | > 0.05 | 13,626 | 14,438 | 16,836 | |
| Not DEGs | │0 ≤ log2FC < 1│ | – | 32,957 | 27,793 | 34,937 |
| ≤ − 1 | > 0.05 | 14,158 | 16,374 | 12,831 | |
| Down-regulated | ≤ − 1 | ≤ 0.001 | 778 | 559 | 2611 |
| ≤ − 1 | 0.001 < P ≤ 0.05 | 2025 | 1904 | 3170 | |
| No data | 2204 | 2204 | 2204 | ||
| Total | 73,833 | 73,833 | 73,833 | ||
The transcripts with log2FC values of ≥ │1│ and FDR p value of ≤ 0.05 were considered to be differentially expressed. The expression levels of transcripts were categorized into upregulation (8085 in IMFvsFF, 10,561 in MFvsFF and 1244 in IMFvsMF), downregulation (2803 in IMFvsFF, 2463 in MFvsFF and 5781 in IMFvsMF), no differential expression (60,741 in IMFvsFF, 58,605 in MFvsFF and 64,604 in IMFvsMF) and no data (2204 in each comparison) between flower sex types based on log2 fold change (≤ − 1 or ≥ 1) and FDR p values (≤ 0.05)
FDR false discovery rate, FC fold change; DEGs differentially expressed genes