Table 1.
Count of isolated cells from human and mouse hearts with different protocols for enzymatic digestion and tissue handling.
| Enzyme digestion protocols | Valid cell objects (no/uL) |
|||
|---|---|---|---|---|
| Human, RNAlater | Mouse, RNAlater | Mouse, snapfrozen | Mouse, fresh | |
| Collagenase type II | 1.3 | 2 | 2.2 | 7.2 |
| Elastase | 0 | 0 | 0 | 2.5 |
| Collagenase type II + Elastase | 0 | 2 | 1 | 2.7 |
| Collagenase type I + Hyaluronidase | 0 | 3 | 4 | 6.1 |
| Liberase | 0.2 | 2 | 3 | 13.2 |
Valid cell objects counted automatically and curated manually based on DRAQ5 DNA staining. Cells were counted automated image analysis in a high-throughput screening microscope.