Figure 4:
Flow cytometric gating scheme for identification of immune cell influx in sinonasal tissue. Eosinophils (Lin−, CD45+, CD11b+, CD11c−, and Siglec-F+), basophils (Lin−, CD45+, CD3−, FcεRIα+, c-Kit−), mast cells (Lin−, CD45+, CD3−, FcεRIα+, c-Kit+). Lineage cocktail: B220, NK1.1, Gr-1, and TER119.