Appendix 1—figure 3. Methods used for evaluating the efficiency of integrase alleles.
(a) scheme of conjugative and chromosomal tests. In both cases the recombination between attI sites produces a selectable phenotype. The dynamic range of each assay is different and so are the results for a given integrase allele. There is a drop of approximately two orders of magnitude between the conjugative and the chromosomal test. The chromosomal assay allowed us to determine the efficiency of highly active mutants that were close to the upper limit (10−1) of the conjugative test. (b) example of recombination rates for intI1 and intI18mut measured in both assays.