Figure 5.

iNKT cell help in the lymph node. Wild-type C57BL/6 mice were transferred with OT-I T cells and one day later injected with 0,172 mg of PLGA nanoparticles (containing 6ug Ovalbumin and 30ng IMM60) via different routes. 24 hours later spleens and lymph nodes were isolated. (a–c) CD40+ percentages of XCR-1+ cDC1 s in (a) spleen, (b) lymph nodes and (c) livers. (d–f) OT-I T cells were analyzed for CD69 expression in (d) spleen, (e) lymph nodes, (f) livers. Each mouse is shown as a dot with mean values. Two-way ANOVA or Kruskal Wallis tests were used for statistical analysis