Figure 7.

Upregulation of ectonucleotidase expression in T cells with effector/memory phenotype and functional properties. Expression of CD39 and CD73 was measured on CD44^highCD62 L⁺ central memory and CD44^highCD62 L⁻ effector memory as well as CD44^lowCD62 L⁺ naïve subsets of total CD8⁺ T cells (A, B) or CD4⁺FoxP3⁻ Tcon (C, D) from spleens of healthy mice by flow cytometry. Data are presented as the percentage of CD39⁺ and CD73⁺ cells within indicated T cell subsets. Spleen CD8⁺ (E, F) or CD4⁺ Tcon (G, H) were stimulated with phorbol 12-myristate 13-acetate and ionomycin for 5 h followed by the detection of intracellular IFN-γ and ectonucleotidase expression on IFN-γ ⁺ or IFN-γ ^– cells (n = 10). Data are presented as the percentage of CD39⁺ and CD73⁺ cells among IFN-γ⁺ and IFN-γ⁻ T cells. **P < .01, ***P < .001