Extended Data Figure 5: Overview of the final adult clusters.

a, Hierarchical clustering tree of our adult clusters, based on the average log-normalized non-integrated expression of the 2,000 most variable genes of the dataset (the genes used to define the unsupervised clusters, Methods). Indicated in blue are the glial clusters. b, Number of cells in our identified neuronal clusters, excluding the T4/T5 cluster that contains 10,780 cells, with unicolumnar neurons in blue and multicolumnar neurons in orange. Importantly, photoreceptors (PR) and lamina neurons L1-L5 clusters contain fewer cells, as these neuronal types were not included in equal proportions in all libraries. As the number of cells per optic lobe for a given neuronal type is rarely formally counted, unicolumnar vs. multicolumnar character is based both on general knowledge and the following references^7,8,12,54.