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. 2021 Jan 9;27(6):417–423. doi: 10.1007/s11655-021-2855-2

Effects of Shenfu Injection (参附注射液) on Inflammatory Response during Post-Resuscitation Myocardial Dysfunction after Cardiac Arrest in Swine

Wei Gu 1, Xiao-min Hou 2, Chun-sheng Li 2,✉
PMCID: PMC7794065  PMID: 33420584

Abstract

Objective

To investigate whether Shenfu Injection (SFI, 参附注射液) can alleviate post-resuscitation myocardial dysfunction by inhibiting the inflammatory response.

Methods

After 8 min of ventricular fibrillation and 2 min of basic life support, 24 pigs were randomly divided into 3 groups (n=8), which were given intravenous bolus injections of SFI (1.0 mL/kg), epinephrine (EP, 0.02 mg/kg) and normal saline (SA), respectively. The animals were sacrificed at 24 h after restoration of spontaneous circulation (ROSC), and serum interleuking-6 (IL-6) and tumor necrosis factor-α (TNF-α) levels were measured by enzyme-linked immunosorbent assay (ELISA); expressions of Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) mRNAs and proteins were determined by RT-PCR and Western blot, respectively.

Results

Compared with the EP and the SA groups, the ultrastructure of myocardial cells were slightly damaged and the systolic function of the left ventricle was markedly improved in the SFI group at 24 h after ROSC (P<0.05). In addition, compared with the EP and SA groups, the SFI group also showed significantly reduced levels of serum IL-6 and TNF-α, protein and mRNA levels of myocardial NF- κB and TLR4 (P<0.05).

Conclusions

Activation of TLR4/NF-κB signaling pathway may be involved in the pathological mechanisms of post-resuscitation myocardial dysfunction. SFI may block NF-κB-mediated inflammatory response by reducing the activity of NF- κB and the level of TNF-α, thus playing a protective role in post-resuscitation myocardial dysfunction.

Keywords: Shenfu Injection, Chinese medicine, cardiopulmonary resuscitation, post-resuscitation myocardial dysfunction, TLR4/NF-κB

Author Contributions

Gu W and Li CS conceived and designed the experiments. Gu W, Zheng PY and Hou XM performed the experiments. Gu W and Hou XM analyzed the data. Hou XM contributed reagents/materials/analysis tools. Gu W and Li CS wrote the paper.

Footnotes

Supported by the Beijing Natural Science Foundation (No. 7182055) and Beijing Municipal Administration of Hospitals Incubating Program (No. 2016022)

Conflict of Interest

The authors declare that they have no conflicts of interest.

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