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. 2021 Jan 1;11(5):2364–2380. doi: 10.7150/thno.47996

Figure 2.

Figure 2

Human BMSC-derived sEVs promote MM cell cycle. (A) MM1S cells were cultured with or without 50 or 100 μg/mL BMSC-derived sEVs for 48 h and the cell cycle was determined using PI staining and flow cytometry. Representative flow cytometry plots are shown in the left panel. The proportions of MM1S cells in different cell cycle stages were measured using flow cytometry and displayed using histogram (right panel). (B) RPMI 8226, (C) U266, or (D) H929 cells were cultured with or without 50 or 100 μg/mL BMSC-derived sEVs for 48 h and the proportions of cells in different cell cycle phases were determined using PI staining and flow cytometry. One representative result in triplicate of three experiments was presented by histograms. Similar results were obtained in three independent experiments. (E) MM1S, RPMI 8226, U266, or H929 cells were cultured with or without 50 or 100 μg/mL DID-labeled BMSC-derived sEVs for 48 h and representative flow cytometry plots are shown. (F) Mean fluorescence intensity of DID in the cells at different cell cycle stages were determined using PI staining and flow cytometry and presented by histograms. One-way ANOVA followed by multiple compressions was used for comparing multiple groups. Error bar, mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.