Fig. 7.

3D projections of 24-h old biomass treated with 10 μg mL⁻¹ tobramycin and stained with live/dead stains. A) The biofilm 1 h after the beginning of treatment. B) The biofilm 3 h after the beginning of treatment. C) The biofilm 5 h after the beginning of treatment. D) The biofilm 17 h after the beginning of treatment. E) Side view of 17-h old treated biofilm, also shown in 5D. Zoom showing the top layer of PI-stained biomass on top of Syto9-stained biomass. F) Fraction of biomass stained green with Syto9. Biomass of both PI- and Syto9-stained cells were quantified, and the part stained with Syto9 was taken as a fraction of the total. G) CFU after 17 h of Tobramycin (10 μg mL⁻¹) treatment or untreated biomass in microtiter wells grown 24, 48, or 72 h before treatment. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)