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Experimental protocol. Experimental protocol used in the present study. Streptozotocin (STZ) was injected s. c.to the 2 days old pups and then allowed to grow under breast milk. At 4 weeks of age the mice were given either normal diet or high-fat diet (HFD) 32. Le Carbone suspension (LC) treatment was given to the NASH + LC group mice from 6 weeks of age to16 weeks of age. All the mice were sacrificed at 16 weeks of age.
