Table 1. Quantification of emx2:Gfp positive cells in wild-type (WT) and emx2 mutant neuromasts.
Genotype | Fish | Neuromast | Total hair cells (HCs) | Gfp+ cells | Anterior Gfp+ HCs |
Posterior Gfp+ HCs |
Mislocated Gfp+ HCs |
---|---|---|---|---|---|---|---|
WT | 7 | 20 | 160 | 78 (49%) |
78/78* (100%) |
0/78 (0%) |
0/78 (0%) |
emx2Gfp/-(loss of function [LOF]) | 3 | 10 | 70 | 33 (47%) |
32/33 (97%) |
1/33 (3%) |
1/33 (3%) |
emx2GOF(F1); emx2Gfp/+ | 10 | 18 | 123 | 30 (24%) |
22/30* (73%) |
8/30 (27%) |
8/30 (27%) |
emx2GOF(F3); emx2Gfp/+ | 29 | 29 | 246 | 100 (41%) |
98/100* (98%) |
2/100 (2%) |
2/100 (2%) |
*All Gfp+ cells are also positive for Emx2 immunostaining.