Ca2+ signaling driving pacemaker activity in submucosal interstitial cells of Cajal in the murine colon

Salah A Baker; Wesley A Leigh; Guillermo Del Valle; Inigo F De Yturriaga; Sean M Ward; Caroline A Cobine; Bernard T Drumm; Kenton M Sanders

doi:10.7554/eLife.64099

. 2021 Jan 5;10:e64099. doi: 10.7554/eLife.64099

Ca²⁺ signaling driving pacemaker activity in submucosal interstitial cells of Cajal in the murine colon

Salah A Baker ^1,^✉, Wesley A Leigh ¹, Guillermo Del Valle ¹, Inigo F De Yturriaga ¹, Sean M Ward ¹, Caroline A Cobine ¹, Bernard T Drumm ^1,^†, Kenton M Sanders ¹

Editors: Mark T Nelson², Richard W Aldrich³

PMCID: PMC7806270 PMID: 33399536

Abstract

Interstitial cells of Cajal (ICC) generate pacemaker activity responsible for phasic contractions in colonic segmentation and peristalsis. ICC along the submucosal border (ICC-SM) contribute to mixing and more complex patterns of colonic motility. We show the complex patterns of Ca²⁺ signaling in ICC-SM and the relationship between ICC-SM Ca²⁺ transients and activation of smooth muscle cells (SMCs) using optogenetic tools. ICC-SM displayed rhythmic firing of Ca²⁺transients ~ 15 cpm and paced adjacent SMCs. The majority of spontaneous activity occurred in regular Ca²⁺ transients clusters (CTCs) that propagated through the network. CTCs were organized and dependent upon Ca²⁺ entry through voltage-dependent Ca²⁺ conductances, L- and T-type Ca²⁺ channels. Removal of Ca²⁺ from the external solution abolished CTCs. Ca²⁺ release mechanisms reduced the duration and amplitude of Ca²⁺ transients but did not block CTCs. These data reveal how colonic pacemaker ICC-SM exhibit complex Ca^2+-firing patterns and drive smooth muscle activity and overall colonic contractions.

Research organism: Mouse

Introduction

Interstitial cells of Cajal (ICC) serve several important functions in the gastrointestinal tract, including generation of pacemaker activity (Langton et al., 1989; Ward et al., 1994; Huizinga et al., 1995), neurotransduction (Burns et al., 1996; Ward et al., 2000a; Sanders et al., 2010), and responses to stretch (Won et al., 2005). Electrical activity in ICC is transmitted to other cells in the tunica muscularis via gap junctions (Komuro, 2006). Smooth muscle cells (SMCs), ICC and another class of platelet-derived growth factor receptor alpha (PDGFRα)-positive interstitial cells are linked together in a coupled network known as the Smooth muscle cells, ICC and PDGFRα⁺ cells (SIP) syncytium (Sanders et al., 2012). The pacemaker function of ICC was deduced from morphological studies (Faussone Pellegrini et al., 1977; Thuneberg, 1982), dissection of pacemaker regions experiments (Smith et al., 1987a; Hara et al., 1986; Serio et al., 1991), studies on isolated ICC and SMCs (Langton et al., 1989; Sanders et al., 2014a; Zhu et al., 2009), studies of muscles from animals with loss-of-function mutations in c-Kit signaling (Ward et al., 1994; Ward et al., 1995; Huizinga et al., 1995) and simultaneous impalements of ICC and SMCs (Cousins et al., 2003). While these experiments were strongly indicative of the obligatory role of ICC as pacemakers in GI smooth muscles and essential role in normal electrical and contractile patterns, no experiments to date have measured pacemaker activity in ICC and responses of SMCs in terms of Ca²⁺ signaling and contraction simultaneously.

The anatomy and distribution of ICC varies from place to place throughout the GI tract. Some areas have only an intramuscular type of ICC (ICC-IM) that are closely aligned with and transduce inputs from excitatory and inhibitory enteric motor neurons (Burns et al., 1996; Beckett et al., 2004; Lies et al., 2014; Sanders et al., 2014b). Other regions contain ICC-IM and pacemaker types of ICC, that exist as a network in the myenteric plexus region of most areas of the gut (ICC-MY) (Rumessen and Thuneberg, 1982; Faussone-Pellegrini, 1992; Komuro et al., 1996; Burns et al., 1997; Komuro, 1999). The colon is more complex in that there are at least four types of ICC, distinguished by their anatomical locations and functions (Ishikawa and Komuro, 1996; Vanderwinden et al., 2000; Vanderwinden et al., 1996; Rumessen et al., 2013). One class of colonic ICC lies along the submucosal surface of the circular muscle (CM) layer (ICC-SM). These cells are known to provide pacemaker activity in colonic muscles, and their activity is integrated with a second frequency of pacemaker activity generated by ICC-MY (Smith et al., 1987a; Smith et al., 1987b; Durdle et al., 1983; Huizinga et al., 1988; Yoneda et al., 2002; Plujà et al., 2001). Pacemaker activities generated by ICC-SM and ICC-MY cause depolarization of SMCs, generation of Ca²⁺ action potentials, and excitation-contraction coupling (Yoneda et al., 2004).

ICC-SM generate slow waves in the canine colon (Smith et al., 1987a; Berezin et al., 1988). These are large amplitude and long-duration events that produce phasic contractions (Keef et al., 1992). The integrity of the ICC-SM network is required for regenerative propagation of slow waves, and disruption of the network causes passive decay of slow waves within a few millimeters (Sanders et al., 1990). Electrical coupling of ICC-SM in a network is an important feature allowing the pacemaker activity to coordinate the electrical activation of SMCs. ICC-SM in proximal colons of rodents also display pacemaker function; however, the frequency of the slow waves is higher (10–22 min⁻¹, mean 14.8. min⁻¹) (Yoneda et al., 2002). Slow waves, in this region of the GI tract, consist of a rapid upstroke phase, 148 mVs⁻¹, that settles to a plateau phase lasting approximately 2 s. The slow waves are coupled to low-amplitude CM contractions (Yoneda et al., 2004). Colonic slow waves have been reported to depend upon both Ca²⁺ entry and intracellular Ca²⁺ release mechanisms; however, Ca²⁺ signaling in colonic pacemaker cells and the coupling of Ca²⁺ events to the electrical responses were not clarified.

Previous studies have shown that all classes of ICC in the GI tract express Ca²⁺-activated Cl^- channels encoded by Ano1 (Gomez-Pinilla et al., 2009; Hwang et al., 2009). This conductance is required for slow wave activity (Hwang et al., 2009), and therefore Ca²⁺ dynamics in ICC are of fundamental importance in understanding pacemaker activity and electrical and mechanical rhythmicity in GI muscles. In the present study, we tested the hypothesis that Ca²⁺ transients in ICC-SM are linked to mechanical activation of the CM and that propagation of activity in ICC-SM is related to and controlled by Ca²⁺ entry via voltage-dependent Ca²⁺ conductances. Experiments were performed on tissues containing ICC-SM taken from mice with cell-specific expression of GCaMP6f in ICC, and changes in intracellular Ca²⁺ were monitored by confocal microscopy and digital video imaging.

Results

ICC-SM distribution within the submucosal plexus

We optimized a preparation in which the submucosal layer was separated from the tunica muscularis. We found that ICC-SM were adherent to the submucosal tissues, so this preparation allowed very clear high resolution of Ca²⁺ transients in ICC-SM in the complete absence of motion artifacts due to muscle contractions. We confirmed the presence and maintenance of ICC-SM networks, which occur in intact muscles, in these preparations.

Kit immunoreactivity revealed a dense network of Kit-positive cells in the submucosal layer of the proximal colon (Figure 1A). The network consisted of ICC-SM interconnected with branching processes (Figure 1B). The average density of cell bodies was 312 ± 33 cells mm^–2 (n = 6), and the average minimum separation between cell bodies was 49.3 ± 2.8 μm (Figure 1A&B; n = 6). Most of the ICC-SM network appeared to be adherent to the submucosal layer of the proximal colon. Isolation of the submucosa by sharp dissection showed that few ICC-SM (1.6 ± 2.1 cells mm^–2; Figure 1C; n = 6) remained adherent to the muscularis in the uppermost region (1–2 μm) of the proximal colon.

Colonic muscles from Kit^+/copGFP mice expressing the copGFP exclusively in ICC were also used to confirm the distribution of ICC-SM. copGFP-positive cells in the submucosal region were present at an average density of 284 ± 27 cells mm^–2 and the average minimum separation between cell bodies was 52.7 ± 2.9 μm (Figure 1D and E; n = 6). ICC-SM were not resolved at the surface of the muscle layer after removing the submucosal layer (Figure 1F; n = 6). Colonic muscles expressing GCaMP6f exclusively in ICC were used to monitor Ca²⁺ signaling in ICC-SM. GCaMP6f-positive cells in the submucosal region were present at an average density of 291 ± 36 cells mm^–2 (n = 10), and the average minimum separation between cell bodies was 50.6 ± 3.8 μm. Representative images of the GCaMP6f-expressing cells in ICC-SM are shown below in the Ca²⁺-imaging experiments (Figure 2A; Figure 3A and Figure 6A).

Figure 2. — Representative dual-color imaging of ICC-SM (green; A) and SMCs (red; B) Ca²⁺ transients were recorded simultaneously from proximal colonic muscles of *Kit-iCre-GCaMP6f/Acta2-RCaMP1.07* mice (see Materials and methods for details). **C and D** Spatiotemporal maps (STMaps) of Ca²⁺ signals in ICC-SM and SMCs during a recording period (gray boxes in **A and B** denote cell locations; see Video 1). The STMaps show coordinated firing of Ca²⁺ transients in both types of cells. Ca²⁺ transient traces are plotted in E (ICC-SM-green, SMCs-red). Tissue displacement was also monitored and plotted (black trace; E). The black dotted arrow depicts the sequence of activation firing of Ca²⁺ transients in ICC-SM, to activation of Ca²⁺ events in SMCs, and tissue displacement. (F) A comparison of latencies (ms) from the start of the initiation of Ca²⁺ transients in ICC-SM to SMC activation and tissue displacement (n = 8). (G) Correlation analysis of latencies between ICC-SM and SMC Ca²⁺ transients and tissue displacement (R² = 0.82). All data graphed as mean ± SEM.

Figure 3. — (A) An image of ICC-SM network from the proximal colon of a Kit-iCre-GCaMP6f mouse visualized at 10x (low resolution) magnification. Scale bar is 100 μm in A and pertains to all images in **B-F**. **A-F** Representative montage of the propagation of a Ca²⁺ wave throughout the ICC-SM network. The yellow arrow in panel C indicates the direction of Ca²⁺ transient propagation. (G) Spatiotemporal map (STMap) of Ca²⁺ signal in single ICC from the movie in panel A showing rhythmic firing of Ca²⁺ waves. Ca²⁺ activity is color coded with warm areas (red, orange) representing bright areas of Ca²⁺ fluorescence and cold colors (blue, black) representing dim areas of Ca²⁺ fluorescence. Summary data of multiple ICC-SM Ca²⁺-firing parameters (n = 15): frequency H, duration I, intensity J, spatial spread K and area of Ca²⁺ transients L. All data graphed as mean ± SEM.

Pacemaker activity of ICC-SM drives responses in SMCs

We developed a new mouse strain (Kit-iCre-GCaMP6f/Acta2-RCaMP1.07) that allowed simultaneous optical measurements of Ca²⁺ transients in ICC-SM and SMCs and tissue displacement (i.e. an optical means of monitoring muscle contractions). The dual-color mouse allowed simultaneous imaging of the two optogenetic Ca²⁺ sensors with different fluorescence characteristics (ensuring minimal spectral overlap). The sensors were expressed in a cell-specific manner in ICC and SMCs to characterize the coordination of signaling between ICC and SMCs (Figure 2 and Video 1). Ca²⁺ imaging of colonic muscles with attached submucosa showed a strong correlation between Ca²⁺ transients in the ICC-SM network and SMCs (Figure 2A–E; n = 8). Ca²⁺ transients recordings were obtained from ICC-SM and SMC at the exact coordinates in the pixel matched images (indicated by the gray box; Figure 2A&B) The sequence of activation began in ICC-SM and spread to SMCs with a latency of 56 ± 14 ms (Figure 2E&F; n = 8). Muscle displacement, an indicator of muscle contraction, was also measured and displayed latencies of 120 ± 17 ms between the rise of Ca²⁺ in SMCs and resolvable displacement (Figure 2E&F; n = 8) and 180 ± 15 ms between activation of Ca²⁺ transients in ICC-SM and contractile displacement (Figure 2E&F; n = 8). In a few instances, the time delay between ICC-SM and SMCs was not readily resolved. This could have resulted from a shift in the site of dominant pacemaker activity to a region outside the field of view (FOV). There was close correlation between the temporal latencies of ICC-SM and SMCs to tissue displacement (Figure 2G; R² = 0.82), indicating the pacemaker nature of ICC-SM, the coupling between ICC-SM and SMCs and the management of Ca²⁺ signaling and contractions in SMCs by the pacemaker activity of ICC-SM.

Video 1. Simultaneous dual-color imaging of submucosal interstitial cells of Cajal (ICC-SM) and smooth muscle cells (SMCs) in the colon.

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A video of propagating Ca²⁺ waves through an ICC-SM network in the proximal colon of Kit-iCre-GCaMP6f/Acta2-RCaMP1.07 strain imaged with a ×20 objective. simultaneous imaging of two optogenetic Ca²⁺ sensors: GCaMP6f in ICC-SM (left field of view [FOV]; green) and RCaMP1.07 in SMCs (right FOV; red) with different fluorescence characteristics (ensuring minimal spectral overlap). The signal coordination between ICC and SMCs showing the correlation between Ca²⁺ transients in the ICC-SM network and activation of SMCs adjacent to ICC-SM. ICC-SM transients (green trace) preceded Ca²⁺ signals in SMCs (red trace). The scale bar (yellow) is 25 µm.

Global Ca²⁺-firing patterns in ICC-SM

Movement generated by muscle contractions is always an issue when imaging cells in muscle preparations in situ. Therefore, we used preparations of ICC-SM adherent to submucosa that were separated from muscle strips in most experiments. At low-magnification (×10), rhythmic Ca²⁺ waves occurred and spread through ICC-SM networks in isolated submucosal preparations. The Ca²⁺ waves occurred at 8–22 cycle min⁻¹ (Figure 3A–G) and averaged 14.9 ± 1.9 cycle min⁻¹ (Figure 3H; n = 15; c = 120). Similar behavior occurred at similar frequencies (i.e. 16.2 ± 1.4 cycle min⁻¹; n = 6) in ICC-SM attached to muscle tissues. Ca²⁺ waves propagated through the isolated ICC-SM networks with velocities of 219 ± 19 mm/s. Ca²⁺ transients imaged at ×10 appeared to be global and had average durations of 2.1 ± 0.4 s (Figure 3I; n = 15; c = 120) and amplitudes of 1.2 ± 0.3 ΔF/F₀ (Figure 3J; n = 15; c = 120). The spatial spread of Ca²⁺ transients was 36.8 ± 0.4 µm (Figure 3K; n = 15; c = 120). The average area occupied by Ca²⁺ transients in the spatiotemporal maps was 51.6 ± 1.6 µm*s (Figure 3L; n = 15; c = 120).

Cell-to-cell propagation of Ca²⁺ transients in ICC‐SM is shown in Figure 3 and Figure 4. Firing of global Ca²⁺ transients appeared to be sequential in nature (Figure 4A–G; n = 10; c = 60), as there was a strong correlation between the occurrence of global Ca²⁺ transients in multiple ICC-SM cells (Figure 4B; n = 10; c = 60). Overlays of STMaps of Ca²⁺ activity in adjacent cells running parallel to each other (Figure 4F&H) showed that the Ca²⁺ transients overlapped (65.4% of total Ca²⁺transients overlapped in the FOV during a 30-s recording period) (Figure 4G). Comparison between intervals of Ca²⁺ firing of multiple ICC-SM showed strong sequential firing as each cell demonstrated very close temporal firing intervals (Figure 4H&I; n = 6; c = 18). These results suggest that Ca²⁺ firing in ICC‐SM networks are entrained and propagate cell‐to‐cell.

ICC-SM Ca²⁺ signals composed of multiple Ca²⁺-firing sites

Low-power imaging suggested global changes in cell Ca²⁺, however, imaging of ICC-SM at higher power (×60–×100) allowed a more detailed visualization of the subcellular nature of the Ca²⁺ transients and revealed complex firing patterns. Imaging of ICC-SM networks with high spatial resolution showed that subcellular Ca²⁺ transients originated from distinct firing sites (Figure 5; n = 25). STMaps constructed from Ca²⁺ transients in single ICC-SM during 30 s of imaging (Figure 5A&B) identified the positions of all frequent firing sites within ICC-SM. Activity plots of individual firing sites showed they can fire once or multiple times during single Ca²⁺ waves (Figure 5B&C). The number of firing sites in a single ICC-SM ranged from 5 to 12 sites with an overall average of 8.2 ± 2 sites/cell (Figure 5D; n = 25).

Figure 5. — (A) Spatiotemporal map (STMap) of Ca²⁺-firing sites from a single ICC-SM during four consecutive firing cycles. Ca²⁺ activity is color coded with warm areas (red, orange) representing bright areas of Ca²⁺ fluorescence and cold colors (blue, black) representing dim areas of Ca²⁺ fluorescence. Nine distinct firing sites were detected in this cell and are marked as color squares on the right of the STMap. (B) Traces of the Ca²⁺ transients at each of the nine Ca²⁺-firing sites shown on the STMap in panel A. (C) 3-D surface plots showing the Ca²⁺ activity at the Ca²⁺-firing sites shown on the STMap in panel A over four consecutive Ca²⁺-firing cycles. (D) Average number of the firing sites per cell (n = 25). Data graphed as mean ± SEM.

We employed Ca²⁺ particle analysis (Drumm et al., 2017) to identify and quantify Ca²⁺-firing sites in ICC-SM. Ca²⁺-firing sites were distinguished based on their spatial and temporal characteristics (Figure 6A–D and Video 2). The firing sites were color coded, as shown in the example in Figure 6D, to visualize and quantify all active sites firing Ca²⁺ transients. The activities of firing sites were plotted as occurrence maps (Figure 6E). Occurrence maps of all firing sites in a region of ICC-SM network demonstrated that the global Ca²⁺ waves resolved at low power resulted from summation of localized Ca²⁺ transients from a multitude of firing sites (Figure 6D–J). Ca²⁺ transients were clustered temporally as Ca²⁺ waves swept through ICC-SM networks (Figure 6E&J). Also apparent from the occurrence maps was that the firing sequence of sites changed from Ca²⁺ wave to Ca²⁺ wave. Not all firing sites discharged Ca²⁺ transients during each wave cycle, and some sites fired more than once (Figure 6E&J). From particle analysis the average particle area/frame of Ca²⁺ transients averaged 3.2 ± 0.4 µm² (Figure 6H; n = 25) and particle count/frame averaged 0.27 ± 0.1 (Figure 6I; n = 25).

Figure 6. — (A) Representative image of an ICC-SM network from proximal colon of a Kit-iCre-GCaMP6f mouse at ×60 magnification. (B) Heat map of total Ca²⁺ PTCLs generated from the video shown in A (see Video 2). (C) Particles were thresholded temporally to generate a heat map indicating Ca²⁺-firing sites in the ICC-SM. (D) Image showing individually color-coded Ca²⁺-firing sites in the field of view (FOV) shown in C. (E) The temporal characteristics of each individual, color-coded firing site is displayed as an occurrence map, with each ‘lane’ representing the occurrence of firing PTCLs within each firing site. Activity traces for PTCLs for the duration of recording from the entire FOV are shown in **F and G**. Traces for PTCL area (F; red) and PTCL count (G; blue) are shown. **H and I** Summary graphs show average PTCL areas and counts for Ca²⁺-firing sites in ICC-SM (n = 25). (J) One Ca²⁺ wave in ICC-SM (white trace) is expanded and the numerous Ca²⁺ initiation sites that fired during this wave are superimposed. (K) Distribution plot showing average percentages of firing sites during a Ca²⁺ wave. Values are calculated for 1 s and plotted in 85 ms bins (n = 25). Data graphed as mean ± SEM.

Video 2. High spatial resolution ICC-SM Ca²⁺ signals composed of multiple Ca^2+-firing sites.

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A video showing subcellular Ca²⁺ transients in ICC-SM at high resolution imaged with a ×60 objective. Ca²⁺ signals were monitored using the genetically encoded Ca²⁺ indicator GCaMP6f. The left panel shows typical stellate-shaped ICC-SM with multiple interconnected processes. The scale bar (yellow) is 10 µm. The middle panel shows the Ca²⁺ particle (PTCL) activity, color coded in blue for raw PTCLs, and the centroids of particles are indicated in purple and green indicates Ca²⁺-firing sites. Note the multiple-site firing of Ca²⁺ transients in ICC-SM. The right panel shows initiation/firing sites accumulation map. The pattern of firing sites Ca²⁺ activity was temporally clustered as Ca²⁺ wave oscillations swept through ICC-SM networks.

Particle analysis also showed that Ca²⁺-firing sites were most active during the first ~256 ms of a Ca²⁺ wave, and activity decayed with time. This point was further illustrated by distribution plots showing the average percentage of firing sites discharging at various times during Ca²⁺ waves (Figure 6K; n = 25). Initially high firing and decay as a function of time suggest that Ca²⁺ entry mechanisms may be important for: (i) initiating Ca²⁺ transients and (ii) organizing the occurrence of Ca²⁺ transients into clusters. It is also possible that Ca²⁺ stores, loading during the diastolic period between Ca²⁺ waves, are more excitable at the onset of each Ca²⁺ wave.

Ca²⁺ influx mechanisms are required for initiation of clustered Ca²⁺ transients in ICC-SM

The effects of reducing extracellular Ca²⁺ ([Ca²⁺]_o) on Ca²⁺ transients was investigated to evaluate the importance of Ca²⁺ influx for the patterning of Ca²⁺ signaling in ICC-SM. Removal of [Ca²⁺]_o abolished Ca²⁺ transients in ICC-SM within 10 min (Figure 7E–L; n = 6). Stepwise reduction in [Ca²⁺]_o (from 2.5 mM to 0 mM) showed that Ca²⁺ transients decreased in a concentration-dependent manner (Figure 7; n = 6). ICC-SM Ca²⁺ PTCL area and count of firing sites were reduced in response to lowering [Ca²⁺] _o (Figure 7F–J; n = 6). Reducing [Ca²⁺]_o from 2.5 mM (control conditions) to 2 mM caused a reduction in the average PTCL area by 19.6 ± 4.5% (Figure 7K; n = 6) and PTCL count by 25.4 ± 2.9% (Figure 7L; n = 6) with no significant change in the duration of PTCLs (Figure 7M; n = 7). Further reduction of [Ca²⁺] _o to 1 mM reduced Ca²⁺ transient parameters by 47.2 ± 3.5% PTCL area (Figure 7K; n = 6) and 45.1 ± 4.4% PTCL count (Figure 7L; n = 6) and showed a significant change in the duration of PTCLs (Figure 7M; n = 7). Lowering [Ca²⁺] _o to 0.5 mM also reduced Ca²⁺ PTCL area by 73.9 ± 3% (Figure 7K; n = 6) and PTCL count by 73.2 ± 2% (Figure 7L; n = 6) with no significant change in the duration of PTCLs (Figure 7M; n = 7). Removal of Ca²⁺ from the extracellular solution (Ca²⁺‐free KRB solution containing 0.5 mM EGTA) abolished Ca²⁺ signals 8–10 min after solution replacement. The Ca²⁺ transient PTCL area was reduced by 99.4 ± 0.6% (Figure 7K; n = 6) and PTCL count by 99.3 ± 0.7% (Figure 7L; n = 6) with no significant change in the duration of PTCLs (Figure 7M; n = 7). We also noted that the highly organized CTCs occurring in the presence of 2.5 mM [Ca²⁺] _o became less organized as [Ca²⁺] _o was reduced (e.g. compare the tight clusters in Figure 7A with the more diffuse clusters in Figure 7C). These experiments highlight the importance of Ca²⁺ entry mechanisms in Ca²⁺ signaling within ICC-SM.

Figure 7. — (A) ICC-SM Ca²⁺ transients and Ca²⁺-firing sites were color coded and plotted as an occurrence map under control conditions with [Ca²⁺]_o = 2.5 mM. (**B–E**) showing the effects of reducing [Ca²⁺]_o to 2 mM B; 1 mM C; 0.5 mM D and after Ca²⁺ removal of [Ca²⁺]_o (0 mM added and final solution buffered with 0.5 mM EGTA) E. **F-J** Traces of Ca²⁺ PTCL activity in ICC-SM (PTCL area, blue and PTCL count, green) under control conditions F and after reducing [Ca²⁺]_o to 2 mM G; 1 mM H; 0.5 mM I and after removal of [Ca²⁺]_o as shown in J. Summary graphs of Ca²⁺ PTCLs in ICC-SM under control conditions and with reduced [Ca²⁺]_o in K (PTCL area); L (PTCL count) and M (PTCL duration). Data were normalized to controls and expressed as percentages (%). Significance was determined using one-way ANOVA, * = p<0.1, ** = p<0.01, **** = p<0.0001, n = 6. All data graphed as mean ± SEM.

Molecular expression of Ca²⁺entry channels in ICC-SM

The apparent dependence on the Ca²⁺ gradient to maintain pacemaker function in ICC-SM suggests that Ca²⁺ entry mechanisms are critical for initiation and organization of CTCs. Therefore, we examined expression of several Ca²⁺ channels that might be responsible for Ca²⁺ entry in ICC-SM (Figure 8). After isolation of the submucosal layer from the proximal colon of Kit^+/copGFP mice and subsequent cell dispersion, we sorted copGFP-positive ICC-SM with fluorescence activated cell-sorting (FACS) and evaluated the expression of voltage-dependent and voltage-independent Ca²⁺ channels by qPCR (Figure 8A&B). First, we confirmed the purity of sorted ICC-SM with cell-specific markers (Figure 8A). Kit receptors and ANO1 channels are signatures of ICC throughout the GI tract and enrichment of Kit and Ano1 expression was observed in sorted ICC-SM compared to unsorted cells (Kit expression was 0.21 ± 0.014; Ano1 expression was 0.14 ± 0.02 relative to Gapdh). The expression levels of Myh11 an SMC marker and Uch11 a pan neuronal marker encoding PGP9.5 were minimal (Myh11 expression was 0.03 ± 0.002; Uch11 expression was 0.002 ± 0.0001 relative to Gapdh), confirming the purity of ICC-SM sorted by FACS.

Figure 8. — (A) Relative expression of cellular-specific biomarker genes in submucosal interstitial cells of Cajal (ICC-SM; sorted to purity by FACS) and compared with unsorted cells dispersed from submucosal tissues obtained from Kit^+/copGFP mice. Relative expression was determined by qPCR and normalized to *Gapdh* expression. Genes examined were *Kit* (tyrosine kinase receptor, found in ICC), *Ano1* (Ca²⁺-activated Cl^- channel), *Uch11* (neural marker encoding PGP 9.5), *Myh11* (smooth muscle myosin). (B) Relative expression of major Ca²⁺ entry channels considered most likely to be expressed in colonic ICC from RNAseq of total ICC in murine colon (Lee et al., 2017). L-Type Ca²⁺ channels (*Cacna1c* and *Cacna1d*) T-type Ca²⁺ channels (*Cacna1g* and *Cacna1h*) and Store‐operated Ca²⁺ entry (SOCE) channels (*Orai1*, *Orai2* and *Orai3*) were evaluated. All data graphed as mean ± SEM (n = 4).

ICC-SM expressed L-type voltage-dependent Ca²⁺ channels encoded by Cacna1c and Cacna1d abundantly (Ca_V 1.2 and Ca_V 1.3 channels, respectively). Cacna1c showed a 0.012 ± 0.0005 and Cacna1d showed 0.066 ± 0.008 relative to Gapdh (Figure 8B; n = 4). ICC-SM also expressed Cacna1h (Ca_V 3.2) and to a lesser extent Cacna1g (Ca_V 3.1), both T-type voltage-dependent Ca²⁺ channels (Figure 8B; n = 4). Cacna1h expression was abundant in ICC-SM (0.07 ± 0.003 relative to Gapdh). Cacna1g expression was less than Cacna1h 0.0014 ± 0.0001 relative to Gapdh (Figure 8B; n = 4).

Maintenance and refilling of cellular Ca²⁺ stores may also be important for mediation and shaping of Ca²⁺ signals in ICC-SM. Store‐operated Ca²⁺ entry (SOCE) has been established as a mechanism for filling stores upon depletion (Putney, 2018; Gibson et al., 1998). Contributions from SOCE via STIM and Orai interactions are essential for maintenance of Ca²⁺ stores in other types of ICC (Zheng et al., 2018; Youm et al., 2019; Drumm et al., 2020a; Drumm et al., 2020b). Colonic ICC-SM showed enrichment in Orai1 and Orai2 but Orai3 was not resolved (Figure 8B; n = 4). Orai1 expression was 0.02 ± 0.001 relative to Gapdh and Orai2 was 0.074 ± 0.008 relative to Gapdh (Figure 8B; n = 4).

L-type Ca²⁺ channels are important for organization of Ca²⁺ transients in ICC-SM

L-type Ca²⁺ channels (Ca_V 1.3, Ca_V 1.2) were expressed in ICC-SM, and Ca²⁺ transients showed dependence on the Ca²⁺ gradient. Previous studies have reported that L-type channel antagonists inhibit slow waves in the colon (Yoneda et al., 2002; Keef et al., 2002). Therefore, we evaluated the contributions of Ca²⁺ entry via L-type channels to Ca²⁺ transients in ICC-SM. Nicardipine (1 μM) abolished Ca²⁺ transients in ICC-SM (Figure 9A&B; n = 8). Firing site occurrence maps (Figure 9C&D; n = 8) describe the inhibitory effects of nicardipine on Ca²⁺ transients. Ca²⁺ PTCL area was reduced to 10.5 ± 4.7% (Figure 9E–G; n = 8) and PTCL count was reduced to 12.3 ± 4.8% (Figure 9H; n = 8). The number of firing sites also decreased to 8.4 ± 3% in the presence of nicardipine (Figure 9I; n = 8). Isradipine inhibits Ca_V 1.2 and Ca_V 1.3 equally (Rüegg and Hof, 1990; Koschak et al., 2001). Isradipine (1 μM) also reduced Ca²⁺ transients in ICC-SM (Figure 9—figure supplement 1A&B; n = 7). ICC-SM Ca²⁺-firing was reduced in the presence of isradipine as shown in the firing sites occurrence maps (Figure 9—figure supplement 1C&D). PTCL area was reduced to 18 ± 5% (Figure 9—figure supplement 1E–G; n = 7), and PTCL count was reduced to 19.5 ± 6% (Figure 9—figure supplement 1H; n = 7). The number of firing sites was inhibited by isradipine to 21.5 ± 6% (Figure 9—figure supplement 1I; n = 7). These data show that ICC-SM Ca²⁺ transients depend upon Ca²⁺ influx via L-type Ca²⁺ channels, and since isradipine had nearly the same or a lesser effect on Ca²⁺ transients than nicardipine, Ca_V 1.2 appear dominant as the Ca²⁺ entry mechanism.

Figure 9. — **A and B** Representative heat-map images of an ICC-SM network from the proximal colon of a Kit-iCre-GCaMP6f mouse showing active Ca²⁺ PTCLs under control conditions and in the presence of nicardipine (1 μM). Ca²⁺ activity is color coded with warm areas (white, red) representing bright areas of Ca²⁺ fluorescence and cold colors (purple, black) representing dim areas of Ca²⁺ fluorescence. Scale bar is 10 μm in both **A and B**. **C and D** Firing sites showing Ca²⁺ activity in ICC-SM. Firing sites were color coded and plotted as an occurrence map under control conditions and in the presence of nicardipine (1 μM). Traces of firing sites showing PTCL area (E; blue) and PTCL count (E; green) under control conditions and in the presence of nicardipine; PTCL area (F; blue) and PTCL count (F; green) show the inhibitory effects of nicardipine on Ca²⁺ transients in ICC-SM. Summary graphs of Ca²⁺ PTCL activity in ICC-SM before and in the presence of nicardipine are shown in G (PTCL area/frame), (H) (PTCL count/frame), (I) the number of PTCL active sites and J (PTCL duration). Data were normalized to controls and expressed as percentages (%). Significance determined using unpaired t-test, ** = p<0.01, **** = p<0.0001, n = 8. All data graphed as mean ± SEM.

Figure 9—figure supplement 1. — **A and B** Representative heat-map images of an ICC-SM network from the proximal colon of a Kit-iCre-GCaMP6f mouse showing active Ca²⁺ PTCLs under control conditions and in the presence of nicardipine (1 μM). Ca²⁺ activity is color coded with warm areas (white, red) representing bright areas of Ca²⁺ fluorescence and cold colors (purple, black) representing dim areas of Ca²⁺ fluorescence. Scale bar is 10 μm in both **A and B**. **C and D** Firing sites showing Ca²⁺ activity in ICC-SM. Firing sites were color coded and plotted as an occurrence map under control conditions and in the presence of nicardipine (1 μM). Traces of firing sites showing PTCL area (E; blue) and PTCL count (E; green) under control conditions and in the presence of nicardipine; PTCL area (F; blue) and PTCL count (F; green) show the inhibitory effects of nicardipine on Ca²⁺ transients in ICC-SM. Summary graphs of Ca²⁺ PTCL activity in ICC-SM before and in the presence of nicardipine are shown in G (PTCL area/frame), (H) (PTCL count/frame), (I) the number of PTCL active sites and J (PTCL duration). Data were normalized to controls and expressed as percentages (%). Significance determined using unpaired t-test, ** = p<0.01, **** = p<0.0001, n = 8. All data graphed as mean ± SEM.

T-type Ca²⁺ channels contribute to Ca²⁺ transients in ICC-SM

T-type Ca²⁺ channels have been identified as the dominant voltage-dependent Ca²⁺ conductance responsible for Ca²⁺ entry and propagation of slow waves in ICC-MY of the small intestine (Kito et al., 2005; Zheng et al., 2014; ). T-type channel antagonists (Ni²⁺ and mibefradil) reduced the rate-of-rise of the upstroke component of the slow waves and higher concentrations attenuated slow wave activity (Kito et al., 2005; Hotta et al., 2007; Yoneda et al., 2003). As reported above, ICC-SM express T-type channels Cacna1h and Cacna1g. Therefore, the role of T-type Ca²⁺ channels in modulating Ca²⁺signaling in ICC-SM was evaluated with specific T-type channel antagonists, NNC 55–0396 (10 μM), TTA-A2 (10 μM) and Z-944 (1 μM). NNC 55–0396 reduced Ca²⁺ transient firing (Figure 10A&B), Ca²⁺ transients firing sites occurrence maps (Figure 10C&D) and firing sites. PTCL area and PTCL count traces show a reduction in Ca²⁺ transient firing (Figure 10E&F). PTCL area was reduced to 31.7 ± 3.3% (Figure 10E–G; n = 9) and PTCL count was reduced to 35.6 ± 4.5% (Figure 10H; n = 9). The number of firing sites was reduced by NNC 55–0396 to 37.6 ± 4.3% (Figure 10I; n = 9). The duration of PTCLs was also reduced by NNC 55–0396 to 73 ± 5.2% (Figure 10J; n = 9). TTA-A2 showed similar inhibitory effects on ICC-SM Ca²⁺ transients (Figure 10—figure supplement 1). PTCL area was reduced to 42 ± 5.8% (Figure 10—figure supplement 1E; n = 7) and PTCL count was reduced to 44.4 ± 6.2% (Figure 10—figure supplement 1F; n = 7) The number of firing sites was also reduced by TTA-A2 to 43.4 ± 6% (Figure 10—figure supplement 1G; n = 7). Z-944 significantly reduced ICC-SM Ca²⁺ transients but was somewhat less effective than NNC 55–0396 or TTA-A2. PTCL area was reduced to 56 ± 10% (Figure 10—figure supplement 1E; n = 5), and PTCL count was reduced to 53 ± 12% (Figure 10—figure supplement 1F; n = 5) The number of firing sites was also reduced by Z-944 to 53.4 ± 11% (Figure 10—figure supplement 1G; n = 5). The data suggest that T-type Ca²⁺ channels also contribute to the initiation and organization of Ca²⁺ transients in ICC-SM.

Figure 10. — **A and B** Representative heat-map images of Ca²⁺ transient particles in ICC-SM under control conditions A and in the presence of NNC 55–0396 (10 μM) (B). Active firing sites were color coded and plotted as an occurrence maps in the ICC-SM network under control C and NNC 55–0396 D conditions. Plots of Ca²⁺ transient particle activity of ICC-SM in control conditions and in the presence of NNC 55–0396 showing PTCL area (blue) and PTCL count (green) under control conditions E and in the presence of NNC 55–0396 F. Summary graphs of average percentage changes in PTCL area G, PTCL count H, the number of PTCL active sites I and PTCL duration J. Data were normalized to controls and expressed as percentages (%). Significance determined using unpaired t-test, **** = p<0.0001, n = 8. All data graphed as mean ± SEM.

Figure 10—figure supplement 1. — **A and B** Representative heat-map images of Ca²⁺ transient particles in ICC-SM under control conditions A and in the presence of NNC 55–0396 (10 μM) (B). Active firing sites were color coded and plotted as an occurrence maps in the ICC-SM network under control C and NNC 55–0396 D conditions. Plots of Ca²⁺ transient particle activity of ICC-SM in control conditions and in the presence of NNC 55–0396 showing PTCL area (blue) and PTCL count (green) under control conditions E and in the presence of NNC 55–0396 F. Summary graphs of average percentage changes in PTCL area G, PTCL count H, the number of PTCL active sites I and PTCL duration J. Data were normalized to controls and expressed as percentages (%). Significance determined using unpaired t-test, **** = p<0.0001, n = 8. All data graphed as mean ± SEM.

Effects of membrane hyperpolarization on Ca²⁺ transients in ICC-SM

Experiments described above showed that Ca²⁺ transients in ICC-SM depend on voltage-dependent Ca²⁺ influx mechanisms (Figures 7–10). The role and contributions of the voltage-dependent channels expressed in ICC-SM (Ca_V 1.3 and Ca_V 1.2 and Ca_V 3.2) was further examined under conditions of membrane hyperpolarization induced by activation of K_ATP channels. K_ATP is functional in colonic SMCs but not in ICC (Huang et al., 2018). Therefore, these experiments were performed on preparations in which ICC-SM remained attached to the muscularis. Pinacidil produces hyperpolarization of SMCs via activation of K_ATP channels (Koh et al., 1998) and the hyperpolarization is expected to conduct to ICC, as they are electrically coupled. Pinacidil (10 μM), a selective K_ATP channel agonist, hyperpolarizes murine colonic muscles by ~10 mV (Kito et al., 2005; Koh et al., 1998; Kito and Suzuki, 2003). Pinacidil had no significant change to Ca²⁺ transient firing, except to reduce the duration of CTCs (Figure 11—figure supplement 1A–L). Ca²⁺ PTCL area was increased to 130.3 ± 24.3% (Figure 11—figure supplement 1G; p value = 0.24; n = 6) and PTCL count was increased to 121.8 ± 17.8% (Figure 11—figure supplement 1H; p value = 0.25; n = 6). The number of firing sites was not affected by pinacidil (Figure 11—figure supplement 1I; p value = 0.22; n = 6). The effects of pinacidil on global Ca²⁺ transients were evaluated. Pinacidil significantly reduced the duration of global Ca²⁺ transients from 1.78 ± 0.2 s to 0.89 ± 0.1 s (Figure 11—figure supplement 1J&K; n = 6). Reduction in the duration of Ca²⁺ transients was associated with a tendency for an increase in frequency, but this parameter did not reach significance. Ca²⁺ oscillation frequency under control conditions was 15.1 ± 1.1 cpm and in the presence of pinacidil was 16.5 ± 1.2 cpm (Figure 11—figure supplement 1J&L; p value = 0.45; n = 6).

The effects of nicardipine were tested in the presence of pinacidil. Under these conditions, nicardipine significantly reduced Ca²⁺ transients in ICC-SM (Figure 11G&H; n = 5). PTCL area was reduced to 38.5 ± 7.6% (Figure 11G; n = 5) and PTCL count was reduced to 42.3 ± 9.1% (Figure 11H; n = 5). In some regards, these results were surprising as membrane potential hyperpolarization might reduce contributions from L-type Ca²⁺ channels (Ca_V 1.2). One explanation is that Ca_V 1.3, which are abundant in ICC-SM and activate at more negative potentials than Ca_V 1.2 (Xu and Lipscombe, 2001) may contribute to Ca²⁺ entry at more hyperpolarized potentials. Further addition of NNC 55–0396 (10 μM) inhibited Ca²⁺ transients in ICC-SM to a greater extent. PTCL area was reduced to 9.0 ± 2% (Figure 11G; n = 5), and PTCL count was reduced to 11.2 ± 1.9% (Figure 11H; n = 5). The utilization of two Ca²⁺ conductances with different ranges of voltage-dependent activation for the initiation of CTCs provides a safety factor that insures persistence of pacemaker activity over a broad range of membrane potentials.

Figure 11. — (A) Ca²⁺-firing sites in ICC-SM are color coded and plotted in an occurrence map in the presence of pinacidil (10 μM). (B) Shows the effects of nicardipine (1 μM) in the continued presence of pinacidil. (C) shows effects of combining nicardipine and NNC 55–0396 (10 μM) in the continued presence of pinacidil. Traces of firing sites PTCL area (blue) and PTCL count (green) under each condition are shown in D pinacidil E pinacidil and nicardipine and F combination of pinacidil, nicardipine, and NNC 55–0396. Summary graphs of Ca²⁺ PTCL activity in ICC-SM in the presence of voltage-dependent Ca²⁺ channel antagonists (nicardipine and NNC 55–0396) are shown in G (PTCL area) and H (PTCL count). (I) The number of Ca^2+-firing events were tabulated during 2 s intervals before the initiation of Ca²⁺ transient clusters in ICC-SM (period of tabulation indicated by the gray box) and summarized in J under control conditions, in pinacidil, in pinacidil and nicardipine and in a combination of pinacidil, nicardipine, and NNC 55–0396. Data were normalized to controls and expressed as percentages (%) in G and H. Significance determined using one-way-ANOVA, * = p<0.1, ** = p<0.01, *** = p<0.001 **** = p<0.0001, n = 5. All data graphed as mean ± SEM.

Figure 11—figure supplement 1. — (A) Ca²⁺-firing sites in ICC-SM are color coded and plotted in an occurrence map in the presence of pinacidil (10 μM). (B) Shows the effects of nicardipine (1 μM) in the continued presence of pinacidil. (C) shows effects of combining nicardipine and NNC 55–0396 (10 μM) in the continued presence of pinacidil. Traces of firing sites PTCL area (blue) and PTCL count (green) under each condition are shown in D pinacidil E pinacidil and nicardipine and F combination of pinacidil, nicardipine, and NNC 55–0396. Summary graphs of Ca²⁺ PTCL activity in ICC-SM in the presence of voltage-dependent Ca²⁺ channel antagonists (nicardipine and NNC 55–0396) are shown in G (PTCL area) and H (PTCL count). (I) The number of Ca^2+-firing events were tabulated during 2 s intervals before the initiation of Ca²⁺ transient clusters in ICC-SM (period of tabulation indicated by the gray box) and summarized in J under control conditions, in pinacidil, in pinacidil and nicardipine and in a combination of pinacidil, nicardipine, and NNC 55–0396. Data were normalized to controls and expressed as percentages (%) in G and H. Significance determined using one-way-ANOVA, * = p<0.1, ** = p<0.01, *** = p<0.001 **** = p<0.0001, n = 5. All data graphed as mean ± SEM.

Inhibiting voltage-dependent Ca²⁺ channels in the presence of pinacidil unmasked underlying Ca²⁺ transients that occurred more randomly than the clustered transients occurring normally (Figure 11F&I). We tabulated the number of Ca²⁺ events in the intervals between CTCs (calculated from a period of 2 s before the onset of a CTC). Underlying Ca²⁺ events were more frequent in the presence of pinacidil and nicardipine and increased again upon addition of NNC 55–0396 (Figure 11J; n = 5).

Contributions of intracellular Ca²⁺ stores and release channels in ICC-SM Ca²⁺activity

Previous studies have demonstrated that Ca²⁺ signaling in ICC-MY in the small intestine depends not only on Ca²⁺ influx but also on Ca²⁺ release from intracellular stores (Drumm et al., 2017). Ca²⁺ release from stores is also critical for generation of pacemaker currents and slow waves (Ward et al., 2000b; Zhu et al., 2015; Bayguinov et al., 2007). The role of Ca²⁺ release mechanisms in Ca²⁺ signaling in ICC-SM was also evaluated. Thapsigargin (1 μM; A SERCA pump antagonist) reduced, but did not block, Ca²⁺ transient firing in ICC-SM (Figure 12A–D). PTCL area was reduced to 29 ± 12% (Figure 12E; n = 6) and PTCL count was reduced to 22 ± 10% (Figure 12F; n = 6). The number of firing sites was reduced by thapsigargin to 21 ± 11% (Figure 12G; n = 6). The duration of PTCLs was increased by thapsigargin to 126.7 ± 9% (Figure 12H; n = 6). Cyclopiazonic acid (CPA, 10 μM), another SERCA antagonist, also reduced Ca²⁺ transient firing (Figure 12I–L). PTCL area was reduced to 36.1 ± 8.3% (Figure 12M; n = 5) and PTCL count was reduced to 29.1 ± 6.3% (Figure 12N; n = 5). The number of firing sites was reduced by CPA to 56 ± 9.5% (Figure 12O; n = 5). There was no significant change in the duration of PTCLs in the presence of CPA (Figure 12P; n = 5).

Figure 12. — (A) Ca²⁺ activity of firing sites in ICC-SM are color coded and plotted in occurrence maps under control conditions and in the presence of thapsigargin (1 μM) (B). Traces of firing sites PTCL area (C; blue) and PTCL count (C; green) under control conditions and in the presence of thapsigargin PTCL area (D; blue) and PTCL count (D; green). Scale bars in C applies to traces in D. Summary graphs of Ca²⁺ PTCL activity in ICC-SM in the presence of thapsigargin are shown in E (PTCL area), (F) (PTCL count), (G) the number of PTCL active sites and H (PTCL duration; n = 6). CPA (SERCA pump inhibitor) reduced transients compared to control as shown in occurrence maps of firing sites **I and J** and Ca²⁺ activity traces **K and L**. Summary graphs of Ca²⁺ PTCL activity in ICC-SM in the presence of CPA are shown in M (PTCL area), (N) (PTCL count), (O) the number of PTCL active sites and P (PTCL duration; n = 5). Data were normalized to controls and expressed as percentages (%). Significance determined using unpaired t-test, * = p<0.1, ** = p<0.01, *** = p<0.001 **** = p<0.0001. All data graphed as mean ± SEM.

ER release channels RyRs and IP₃Rs amplify and sustain Ca²⁺ signaling via direct localized release of Ca²⁺ or Ca²⁺-induced Ca²⁺ release (CICR) (Kaßmann et al., 2019; Vierra et al., 2019; Amberg et al., 2006; Takeda et al., 2011; Fill and Copello, 2002; Dupont and Goldbeter, 1994; Thomas et al., 1996; Berridge, 2009). Contributions from RyRs and IP₃Rs to Ca²⁺ transients in ICC-SM were therefore investigated. Ryanodine (100 μM) significantly reduced Ca²⁺ event firing in ICC-SM (Figure 13A–F). PTCL area was reduced to 76.1 ± 1.6% (Figure 13G; n = 4) and PTCL count was reduced to 80.9 ± 2.7% (Figure 13H; n = 4). The number of firing sites was also reduced by ryanodine to 75.7 ± 2.1% (Figure 13I; n = 4). There was no significant change in the duration of PTCLs in the presence of ryanodine (Figure 13J; n = 5). We also noted that the greatest effects of ryanodine on Ca²⁺ transients occurred after the first ~300 ms of CTCs (Figure 13K&L; n = 4). Thus, ryanodine shortens the durations of the CTCs. Ca²⁺ release mechanisms via RyRs contribute to the overall patterns of Ca²⁺ waves in ICC-SM, as shown by the distribution plots of average percentages of firing sites during a Ca²⁺ wave (Figure 13L; n = 4).

Xestospongin C (10 μM; An IP₃R antagonist) also reduced Ca²⁺ events in ICC-SM (Figure 13—figure supplement 1A–I; n = 4). PTCL area was significantly reduced to 78 ± 5.8% (Figure 13—figure supplement 1G; n = 4) and although both PTCL count and number were reduced to 75.3 ± 10.5% and 74.8 ± 9.3%, respectively, these effects did not reach statistical significance (PTCL count: p value = 0.08 and PTCL number: p value = 0.06; Figure 13—figure supplement 1H&I; n = 4). Xestospongin C displayed inhibitory effects similar to ryanodine; most of the inhibition of Ca²⁺ events occurred after the first ~400 ms of CTCs (Figure 13—figure supplement 1J&K; n = 4). Thus, Ca²⁺ release via IP₃Rs also contributes to the overall pattern of Ca²⁺ waves in ICC-SM, as shown by the distribution plots of average percentages of firing sites during a Ca²⁺ wave (Figure 13—figure supplement 1K; n = 4).

2-APB (100 μM) and tetracaine were also tested (100 μM; Figure 13—figure supplement 2A&B; n = 5) as secondary tests of the contributions of IP₃Rs and RyRs in CTCs. 2-APB reduced Ca²⁺ PTCL area to 61.1 ± 14.5% (Figure 13—figure supplement 2 Ai; n = 5) and reduced PTCL count to 58 ± 13.2% (Figure 13—figure supplement 2 Aii; n = 5). The number of firing sites was also reduced by 2-APB to 63.7 ± 11.3% (Figure 13—figure supplement 2 Aiii; n = 5). Tetracaine reduced Ca²⁺ PTCL area to 85 ± 5.1% (Figure 13—figure supplement 2 Bi; n = 5) and reduced PTCL count to 69.5 ± 7.8% (Figure 13—figure supplement 2 Bii; n = 5). The number of firing sites was also reduced by tetracaine to 71.3 ± 5.9% (Figure 13—figure supplement 2 Biii; n = 5).

The effects of 2-APB could be non-specific and may include effects on store-operated Ca²⁺ entry channels (SOCE; e.g. by blocking Orai channels). Previous studies have shown SOCE to be important for maintenance of Ca²⁺ stores and sustaining Ca²⁺ release from the ER (Zheng et al., 2018; Lyfenko and Dirksen, 2008; Trebak et al., 2013; Chen and Sanderson, 2017; Putney, 2011; Prakriya and Lewis, 2015). ICC-SM express Orai channels (Orai1 and Orai2; Figure 8B), so the role of SOCE in maintenance of Ca²⁺ transients was examined using an Orai antagonist. GSK 7975A (10 μM; An Orai antagonist) reduced the firing frequency of CTCs (Figure 14A&B). Firing site occurrence (Figure 14C&D) and PTCL counts and areas were reduced (Figure 14E&F). Ca²⁺PTCL area was reduced to 42.4 ± 9.4% (Figure 14E–G; n = 7), and PTCL count was reduced to 48 ± 7% (Figure 14H; n = 7). The number of firing sites was also inhibited by GSK 7975A to 47.5 ± 4.1% (Figure 14I; n = 7). There was no significant change in the duration of PTCLs in the presence of GSK 7975A (Figure 14J; n = 7).

Figure 14. — (A) Representative Heat-map image of an ICC-SM network showing total active Ca²⁺ PTCLs under control conditions and in the presence of GSK-7975A (10 μM, for 20 min) B. **C and D** Ca²⁺-firing sites are color coded and plotted in occurrence maps showing the effect of the SOCE channel antagonist, GSK-7975A (100 μM), on ICC-SM Ca²⁺ transients. Traces of PTCL area (E; blue) and PTCL count (E; green) under control conditions and in the presence of GSK-7975A, PTCL area (F; blue) and PTCL count (F; green). Summary graphs of Ca²⁺ PTCL activity in ICC-SM in the presence of GSK-7975A are shown in G (PTCL area), (H) (PTCL count), (I) the number of PTCL active sites, and J (PTCL duration; n = 7). Significance determined using unpaired t-test, **** = p<0.0001. All data graphed as mean ± SEM.

Discussion

This study characterized Ca²⁺ transients responsible for the pacemaker function of ICC-SM that contributes to contractile patterns in colonic motility. The sequence of activation from ICC-SM to colonic SMCs was quantified using two optogenetic sensors, expressed specifically in ICC or SMCs. Correlation analysis demonstrated a 1:1 relationship between CTCs in ICC-SM and Ca²⁺ signaling and contractile responses in SMCs. The CTCs in ICC-SM consisted of ~2 s bursts of activity from multiple sites within cells. Organization of the Ca²⁺ transients into clusters was due to voltage-dependent Ca²⁺ entry that appeared to be due to activation of both high-voltage activated Ca²⁺ channels (L-type encoded by Cacna1c and Cacna1d in ICC-SM) and low-voltage activated Ca²⁺ channels (T-type encoded by Cacna1h and possibly Cacna1g in ICC-SM). A portion of the Ca²⁺ transients making up CTCs were due to Ca²⁺ entry, as the earliest Ca²⁺ transients resolved in a cluster were not blocked by thapsigargin, CPA or antagonists of ryanodine and IP₃ receptors. The earliest events were blocked by nicardipine, suggesting that L-type Ca²⁺ channels are the dominant Ca²⁺ entry pathway at basal resting potentials. Ca²⁺ transients in ICC-SM were not as sensitive to block by STIM and Orai, as in other ICC (Zheng et al., 2018; Drumm et al., 2019a), However, the Orai antagonist reduced the frequency of CTCs and may have blocked these events completely with longer treatment periods. Our data show that Ca²⁺ entry is fundamental in ICC-SM Ca²⁺ transients. In fact, localized Ca²⁺ influx via clusters of L-type Ca²⁺ channels may cause localized elevation in [Ca²⁺]_i and activation of ANO1 channels directly. Localized Ca²⁺ entry events through L-type Ca²⁺ channels have been termed Ca²⁺sparklets, and these events could be involved in Ca²⁺ influx (Santana et al., 2008; Sonkusare et al., 2012; Navedo and Amberg, 2013a) and initiation of pacemaker activity in ICC-SM. However, the decrease in the frequency of CTCs by manipulations to reduce stored Ca²⁺ also suggest an important role for Ca²⁺ release in pacemaker activity, perhaps resulting from coupling between Ca²⁺ entry and CICR (Navedo and Santana, 2013b).

In this study, we developed a new preparation in which ICC-SM adherent to the submucosa was used to allow very high-resolution imaging without complications from muscular contractions. In situ preparations of this type in which the natural structure and connectivity between ICC are maintained may be valuable for future studies of cellular mechanisms responsible for pacemaker activity and factors that regulate or degrade pacemaker activity in pathophysiological conditions.

The pacemaker function of ICC-SM was demonstrated in a novel manner by simultaneous two-color optogenetic imaging with green (GCaMP6f) and red (RCaMP1.07) Ca²⁺ sensors expressed in ICC and SMCs, respectively. Imaging in this manner revealed the sequence of activation in ICC-SM and SMCs, showing clearly the frequency, onset and duration of Ca²⁺ transients in ICC-SM, the spatial spread of Ca²⁺ transients in ICC-SM networks, the development of Ca²⁺ transients in SMCs and tissue displacement (i.e. an optical indicator of muscle contraction). Correlation analysis demonstrated the coherence of these events. Ca²⁺ transients, lasting for about 2 s, propagated without decrement through networks of ICC-SM and preceded and likely initiated Ca²⁺ signaling and contractions in SMC, as was also suggested by intracellular microelectrode recordings from cells along the innermost surface of canine colonic muscles (Sanders et al., 1990). ICC-SM are electrically coupled to each other and the network of ICC-SM is coupled to SMCs via gap junctions, providing a means of electrical communication. Ca²⁺ transients initiate depolarization of ICC-SM via activation of ANO1 channels, depolarizing currents conduct to SMCs, and depolarization of SMCs activates Ca²⁺ entry, by increasing the open probability of L-type Ca²⁺ channels, and excitation-contraction coupling. Depolarizing signals from multiple ICC-SM influence the excitability of SMC, and the overall depolarization driving SMCs results from the summation of activity from the ICC-SM network.

Slow waves with characteristics similar to those found in the stomach and small intestine (i.e. relatively fast upstroke depolarization and a plateau phase) are generated along the submucosal surface of the CM layer in the colon (Smith et al., 1987a). It was discovered that peeling the submucosa from the innermost surface of CM blocked slow waves (Durdle et al., 1983). While the authors of that study thought this tissue was mostly connective tissue with possibly some adherent SMCs, it became clear that a population of pacemaker cells, ICC-SM, are present along the submucosal surface (Berezin et al., 1988; Ward et al., 1991). In the present study, we found that ICC-SM and the networks they form are preserved and remain functionally similar in isolated submucosal tissues to ICC-SM attached to the muscularis. It should be noted that ICC-SM were more adherent to CM in the distal colon, and it was more difficult to obtain ICC-SM/submucosal preparations from that region. Submucosal tissues with adherent ICC-SM were used in the current study to eliminate movement artifacts generated by muscle contractions that plague high-resolution Ca²⁺ imaging in most smooth muscle tissues.

While the frequency of pacemaker activity was relatively stable over time in a given preparation, the sequence of activation of individual ICC-SM within the network varied as a function of time, as previously observed in gastric (Hennig et al., 2004) and small intestinal (Park et al., 2006) ICC-MY networks. What appeared as global Ca²⁺ transients in low resolution imaging partitioned into clusters of localized Ca²⁺ transients when viewed with a ×60 objective at 30 frames/s or at higher acquisition speeds. Summation of the clustered events reproduced the frequency and duration of the Ca²⁺ waves observed at low resolution. Multiple firing sites, averaging ~8 per cell, were identified. This pattern of clustered Ca²⁺ transients (CTCs) was also observed in ICC-MY of the small intestine, the pacemaker cells in that region (Drumm et al., 2017). Organization of Ca²⁺ transients into clusters in ICC-SM was dependent upon voltage-dependent Ca²⁺ entry, and our data revealed that in contrast to ICC-MY of the small intestine, Ca²⁺ entry by both dihydropyridine-sensitive and insensitive mechanisms contributes to clustering and propagation of Ca²⁺ waves in intact networks. Nicardipine and isradipine reduced the occurrence of CTCs dramatically, and the T-channel antagonists, NNC 55–0396, TTA-A2, and Z-944 also reduced the occurrence and disordered the Ca²⁺ transients. Cacna1c, Cacna1d, and Cacna1h were expressed in purified ICC-SM, and the presence and function of these channels can explain the pharmacological observations. Channels resulting from Cacna1d (encoding Ca_Vα1D) activate at relatively hyperpolarized membrane potentials and their currents are partially inhibited by dihydropyridines (~50–70% of current density block) in comparison to Cacna1c gene products (Ca_Vα1C) (Xu and Lipscombe, 2001; Bell et al., 2001), but isradipine blocks Ca_Vα1C and Ca_Vα1D equally (Anekonda et al., 2011; Berjukow et al., 2000; Scholze et al., 2001). The fact that isradipine had no greater effect on the occurrence of clustered Ca²⁺ transients than nicardipine suggests that the L-type component of Ca²⁺ entry may be carried primarily by Ca_Vα1C channels. We have observed relatively robust expression of Cacna1d in a variety of ICC in mice (Drumm et al., 2017; Lee et al., 2017); however, the function of these channels has not been identified specifically.

Having three independent voltage-dependent Ca²⁺ conductances with different voltage-dependent properties coordinate clustering of Ca²⁺ transients provides a safety factor for preservation of pacemaker activity over a broad range of membrane potentials. In spite of overarching changes in membrane potential that might influence the availability of ion channels with narrow voltage-ranges, the broader range of activation potentials offered by expression and function of both L-type and T-type Ca²⁺ channels might protect against voltage-dependent inhibition of pacemaker activity. L-type channels are activated at less polarized potentials than T-type channels (Nowycky et al., 1985). Thus, a factor producing tonic hyperpolarization of the SIP syncytium (e.g. purinergic inhibitory neurotransmission; Gallego et al., 2008; Hwang et al., 2012) may tend to switch the dominant voltage-dependent Ca²⁺ entry mechanism from L-type to T-type Ca²⁺ channels. This concept was demonstrated by the decreased inhibitory effects of nicardipine and increased effects of NNC 55–0396 on Ca²⁺ transients after exposure of tissues to pinacidil. The opposite might be true if the SIP syncytium experiences a depolarizing influence (e.g. neurogenic or humerogenic).

Pinacidil hyperpolarizes colonic muscles through activation of K_ATP channels in SMCs (Koh et al., 1998). This compound increased the frequency and decreased the duration of CTCs. These results are consistent with the effects of pinacidil on electrical pacemaker activity in the small intestine where it increases the dV/dt_max of the upstroke depolarization and decreases the duration of slow waves (Kito et al., 2005). The increase in frequency may have been due to reduced inactivation and increased availability of Ca_Vα1D and T-type channels (Ca_Vα1H) at more hyperpolarized potentials. The decrease in the duration of CTCs may be due to a relative shift in the importance of T-type vs. L-type Ca²⁺ channels with hyperpolarization. In the presence of pinacidil, NNC 55–0396 had increased antagonistic effects on CTCs. Ca²⁺ currents via T-type channels inactivate rapidly, whereas L-type channel inactivation is slower and incomplete (Hirano et al., 1989; Tseng and Boyden, 1989; Xu and Best, 1992). Thus, the Ca²⁺ entry period for T-type currents is likely to be more transient than with L-type currents. Channel density in proximity to Ca²⁺ release channels may also affect the degree of coupling between Ca²⁺ entry and CICR, but, as yet, little is known about the structure and functional components of microdomains in ICC.

The importance of Ca²⁺ entry as the primary means of activation and organization of pacemaker activity in ICC-SM was shown by the discoordination of Ca²⁺ transients when extracellular Ca²⁺ was decreased and the incomplete effects of thapsigargin and CPA on Ca²⁺ transients. We noted tight clustering of Ca²⁺ transients at 2.5 and 2.0 mM [Ca²⁺]_o but the tightness of the CTCs disassociated when the driving force for Ca²⁺ entry (i.e. [Ca²⁺]_o was reduced to 1 mM), and frequency of CTCs was greatly reduced at concentrations lower than 1 mM. Our concept is that Ca²⁺ entry couples to CICR in ICC. Reducing the driving force for Ca²⁺ entry would be expected to reduce the probability for effective coupling to CICR. CICR would be negligible when Ca²⁺ entry falls below threshold levels. Concentrations of thapsigargin and CPA that blocked Ca²⁺ transients quantitatively in other ICC (Drumm et al., 2020a; Drumm et al., 2019a; Drumm et al., 2017) caused a partial block of Ca²⁺ transients in ICC-SM. In fact, these drugs caused a marked narrowing of the duration of the CTCs, and this led us to analyze the temporal characteristics of Ca²⁺ transients within clusters. Ca²⁺ transients at the beginning of the CTCs were unaffected by ryanodine and xestospongin C, but transients toward the end of the clusters were blocked. These data suggest that the initial Ca²⁺ transients may result primarily from Ca²⁺ entry, and CTCs are sustained by Ca²⁺ release. This concept is also supported by previous studies showing inhibition of slow waves by blocking Ca²⁺ entry (L-type and T-type Ca²⁺ channels) (Drumm et al., 2017).

We have searched for a preparation of pacemaker ICC that would allow us to investigate the underlying pacemaker activity. We have speculated that stochastic Ca²⁺ release events, occurring in all ICC (Sanders et al., 2014a), are responsible for the spontaneous transient depolarizations (STDs) observed in patch clamp recordings from isolated ICC (Zhu et al., 2009; Zhu et al., 2011). No simultaneous recordings of Ca²⁺ transients and membrane currents or potentials changes have been achieved yet, and the expected link between Ca²⁺ transients and STDs is based on the fact that these events have common pharmacology and sensitivity to drugs that interfere with Ca²⁺ release (Zhu et al., 2015). Ca²⁺ transients and the spontaneous transient inward currents (STICs) due to activation of ANO1 channels result in spontaneous transient membrane depolarizations (STDs). Temporal summation of STDs is likely to be the generator potentials that activate T-type or L-type Ca²⁺ currents and initiate propagating slow wave events. In this concept it is logical to suggest that inhibition of Ca²⁺ release should reduce the duration of the CTCs, and inhibition of Ca²⁺ entry should inhibit the organizing influence of Ca²⁺ entry and block CTCs. When CTCs are blocked, stochastic Ca²⁺ transients may be unleashed, as occur in ICC-IM (Drumm et al., 2019a) and ICC-DMP (Baker et al., 2016) that lack expression of voltage-dependent Ca²⁺ entry mechanisms. Block of CTCs and unmasking of stochastic Ca²⁺ transients was accomplished by hyperpolarization with pinacidil and reduction in the availability of L-type and T-type Ca²⁺ channels with nicardipine and NNC 55–0396. ICC-SM, as imaged in the current experiments provide a potent model for investigating basic pacemaker mechanisms and what happens to these events in response to neurotransmission, hormonal and paracrine inputs and pathological or inflammatory conditions.

Previous studies have supported a role for store-operated Ca²⁺ entry (SOCE) in maintaining Ca²⁺ release events in ICC (Zheng et al., 2018; Drumm et al., 2019a). This is logical because Ca²⁺ release is extremely dynamic in ICC, and it is likely that Ca²⁺ stores would be depleted without an effective recovery mechanism. SOCE depends upon the expression of Orai channels and the ER delimited activator of Orai, STIM, that senses ER Ca²⁺ and binds to and activates Orai channels when Ca²⁺ depletion of the ER occurs (Butorac et al., 2020). STIM and Orai are expressed in colonic ICC (Lee et al., 2017). However, an antagonist of Orai, GSK7975A, reduced the frequency of CTCs, but failed to block these events at a concentration effective in blocking Ca²⁺ transients in small intestinal ICC-MY (Zheng et al., 2018). STIM/Orai interactions appear to have a role in Ca²⁺ store maintenance in ICC-SM, but our data suggest that Ca²⁺ entry via L-type and T-type Ca²⁺ channels also provide Ca²⁺ entry mechanisms that may contribute to store refilling. It could also be suggested that GSK7975A, a well-known antagonist for Orai1 (IC₅₀ = 4 μM; Derler et al., 2013), is less effective on Orai2, the dominant isoform expressed in ICC-SM. However, studies on cortical neurons that express only Orai2 showed about 50% block of SOCE by GSK7975A (5 μM) (Chauvet et al., 2016).

In summary ICC-SM, as suggested from dissection and electrophysiological experiments (Smith et al., 1987a), are pacemaker cells distributed in an electrically coupled network along the submucosal surface of the CM layer. Our experiments demonstrate that Ca²⁺ transients in ICC-SM couple to activation of Ca²⁺ transients and contractions in neighboring SMCs. The contractile events, called ‘ripples’ by some authors in describing integrated colonic contractions (Corsetti et al., 2019; D'Antona et al., 2001), summate with the larger amplitude contractions emanating from the myenteric region of the tunica muscularis to produce mixing and propagated movements characteristic of colonic motility (Rosli et al., 2020). Data from this study suggest that voltage-dependent Ca²⁺ entry serves at least four important functions in the pacemaker activity of ICC-SM: (i) Propagation of activity within the ICC-SM network depends upon voltage-dependent Ca²⁺ entry, and the functions and voltage-dependent properties of three types of Ca²⁺ conductances appear to provide a safety factor that tends to preserve pacemaker activity over a broad range of membrane potentials (see Figure 15 and Figure 15—figure supplement 1). (ii) Ca²⁺ entry is the mechanism that organizes Ca²⁺ release events into CTCs. These events constitute the Ca²⁺ waves that propagate through ICC-SM networks, and cause slow wave depolarizations by activation of ANO1 channels. (iii) Ca²⁺ entry also appears to contribute to refilling of stores, as pacemaker activity was not as immediately dependent upon SOCE as in other ICC (Zheng et al., 2018; Drumm et al., 2019a). (iv) The observations that treatments expected to reduce Ca²⁺ release from stores and reduce coupling between Ca²⁺ entry and CICR reduced, but did not block CTCs, may indicate that transient Ca²⁺ entry (sparklets), possibly through activation of ANO1 channels and depolarization, may underlie the pacemaker functions of ICC-SM. Additional studies will be necessary to resolve these hypotheses in finer detail. The preparation of excised submucosal tissue with adherent ICC-SM removes movement artifacts from imaging and is likely to provide a powerful tool for improving resolution of pacemaker mechanisms and determining how regulatory and pathophysiological factors affect basic pacemaker mechanisms.

Figure 15. — (A) Shows segments of plasma membrane (PM) and endoplasmic reticulum membrane (ER) that form PM-ER junctions and microdomains. At least three types of voltage-dependent Ca²⁺ channels (VDCC) are expressed in ICC-SM, Ca_V1.2, Ca_V1.3, and Ca_V3.2. These conductances, with voltage-dependent activation and inactivation properties spanning a broad range of negative potentials, insure maintenance of pacemaker activity under conditions of hyperpolarization or depolarization in ICC-SM. Pacemaker activity (1. Initial events) in ICC-SM could be due to spontaneous release of Ca²⁺ from stores in the ER and utilize either IP₃R or RYR receptors or both (Ca²⁺ sparks and puffs). However, our data cannot rule out the possibility that transient openings of voltage-dependent Ca²⁺ channels (sparklets) and amplification of Ca²⁺ in microdomains by CICR constitute the initial events of pacemaker activity. In this case, Ca²⁺ release from stores is not the primary pacemaker event but a secondary response to Ca²⁺ entry. Inhibition of Ca²⁺ release from stores would lead to reduced probability of CICR and decrease the frequency of CTCs. Our hypothesis is that Ca²⁺ entry and/or release from stores activates Ca²⁺-dependent Cl^- current due to ANO1 channels in the plasma membrane. Active propagation between cells in interstitial cells of Cajal (ICC) networks (Phase 2) was inhibited by blocking voltage-dependent Ca²⁺ channels. Active propagation may also require or depend upon amplification of Ca²⁺ in microdomains by CICR. The duration of Ca²⁺ entry is likely to be brief due to voltage-dependent inactivation of L- and T-type Ca²⁺ channels. The duration of CTCs appears to be enhanced by CICR (Phase 3). Our data show that the duration of CTCs is reduced by several manipulations known to inhibit Ca²⁺ release from stores. In Phase four store reloading may occur by multiple mechanisms and may include: (i) transient Ca²⁺ entry via sparklets, (ii) activation of SOCE via STIM/ORAI interactions, and (iii) the increase in Ca²⁺ entry that occurs via depolarization and activation of Ca²⁺ entry at the onset of each CTC. (B) A novel hypothesis emerges from this study suggesting that the pacemaker mechanism in non-voltage-clamped cells includes a cyclical, positive-feedback phenomenon that may be responsible for initiation of CTCs and relies on: (i) Ca²⁺ entry through voltage-dependent Ca²⁺ channels. Openings of clusters of these channels would generate sparklets; (ii) Ca²⁺ entry initiates CICR which amplifies [Ca²⁺]_i within microdomains; (iii) the rise in [Ca²⁺]_i activates ANO1 channels in the PM causing depolarization; (iv) depolarization enhances the open probability of voltage-dependent Ca²⁺ channels, increasing Ca²⁺ entry. This cycle creates positive feedback for Ca²⁺ entry, clustering of localized Ca²⁺ transients due to Ca²⁺ entry during the first 350–450 ms of CTCs and development of slow wave depolarizations in ICC-SM.

Figure 15—figure supplement 1. — (A) Shows segments of plasma membrane (PM) and endoplasmic reticulum membrane (ER) that form PM-ER junctions and microdomains. At least three types of voltage-dependent Ca²⁺ channels (VDCC) are expressed in ICC-SM, Ca_V1.2, Ca_V1.3, and Ca_V3.2. These conductances, with voltage-dependent activation and inactivation properties spanning a broad range of negative potentials, insure maintenance of pacemaker activity under conditions of hyperpolarization or depolarization in ICC-SM. Pacemaker activity (1. Initial events) in ICC-SM could be due to spontaneous release of Ca²⁺ from stores in the ER and utilize either IP₃R or RYR receptors or both (Ca²⁺ sparks and puffs). However, our data cannot rule out the possibility that transient openings of voltage-dependent Ca²⁺ channels (sparklets) and amplification of Ca²⁺ in microdomains by CICR constitute the initial events of pacemaker activity. In this case, Ca²⁺ release from stores is not the primary pacemaker event but a secondary response to Ca²⁺ entry. Inhibition of Ca²⁺ release from stores would lead to reduced probability of CICR and decrease the frequency of CTCs. Our hypothesis is that Ca²⁺ entry and/or release from stores activates Ca²⁺-dependent Cl^- current due to ANO1 channels in the plasma membrane. Active propagation between cells in interstitial cells of Cajal (ICC) networks (Phase 2) was inhibited by blocking voltage-dependent Ca²⁺ channels. Active propagation may also require or depend upon amplification of Ca²⁺ in microdomains by CICR. The duration of Ca²⁺ entry is likely to be brief due to voltage-dependent inactivation of L- and T-type Ca²⁺ channels. The duration of CTCs appears to be enhanced by CICR (Phase 3). Our data show that the duration of CTCs is reduced by several manipulations known to inhibit Ca²⁺ release from stores. In Phase four store reloading may occur by multiple mechanisms and may include: (i) transient Ca²⁺ entry via sparklets, (ii) activation of SOCE via STIM/ORAI interactions, and (iii) the increase in Ca²⁺ entry that occurs via depolarization and activation of Ca²⁺ entry at the onset of each CTC. (B) A novel hypothesis emerges from this study suggesting that the pacemaker mechanism in non-voltage-clamped cells includes a cyclical, positive-feedback phenomenon that may be responsible for initiation of CTCs and relies on: (i) Ca²⁺ entry through voltage-dependent Ca²⁺ channels. Openings of clusters of these channels would generate sparklets; (ii) Ca²⁺ entry initiates CICR which amplifies [Ca²⁺]_i within microdomains; (iii) the rise in [Ca²⁺]_i activates ANO1 channels in the PM causing depolarization; (iv) depolarization enhances the open probability of voltage-dependent Ca²⁺ channels, increasing Ca²⁺ entry. This cycle creates positive feedback for Ca²⁺ entry, clustering of localized Ca²⁺ transients due to Ca²⁺ entry during the first 350–450 ms of CTCs and development of slow wave depolarizations in ICC-SM.

Materials and methods

Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Antibody	Anti- c-Kit (Goat polyclonal)	R and D Systems	Cat# AF1356, RRID:AB_354750	IHC (1:500)
Antibody	Alexa-488 (donkey anti-goat IgG)	Invitrogen/ Thermo Fisher Scientific	(Cat# A32814, RRID:AB_2762838)	IHC (1:1000)
Sequence-based reagent	Kit, Ano1, Myh11,Uchl1, Cacna1c Cacna1d, Cacna1g Cacna1h Orai1,Orai Orai3	This paper	PCR primers	Suppl. Table 1
Chemical compound, drug	NNC 55–0396, TTA-A2	Alomone Labs	Cat# N-206 Cat# T-140
Chemical compound, drug	2-APB, tetracaine, nicardipine, pinacidil, EGTA	Millipore-Sigma	Cat# D9754; Cat# T7508; Cat# N7510; Cat# P154; Cat# E4378
Chemical compound, drug	Thapsigargin, isradipine, Z-944, CPA, ryanodine	Tocris Bioscience	Cat# 1138/1; Cat# 2004/10; Cat# 6367/10; Cat# 1235/10; Cat# 1329/1
Chemical compound, drug	GSK 7975A	Aobious	Cat# AOB4124
Chemical compound, drug	Xestospongin C (XeC)	Cayman Chemical	Cat# 64950
Software, algorithm	STMapAuto, Ca²⁺ Analysis Software	https://github.com/gdelvalle99/STMapAuto	https://doi.org/10.1016/j.ceca.2020.102260

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Animals

Kit^+/copGFP mice (B6.129S7-^{Kittm1Rosay/J}; 5–8 wk old) were bred in house (Ro et al., 2010). GCaMP6f-floxed mice (Ai95 (RCL-GCaMP6f)-D) and C57BL/6 mice, their wild-type siblings, were purchased from Jackson Laboratories (Bar Harbor, MN, USA). Kit-iCre mice (c-Kit^+/Cre-ERT2) were gifted from Dr. Dieter Saur (Technical University Munich, Munich, Germany).

Generation of Kit-iCre-GCaMP6f/Acta2-RCaMP1.07 mice

Acta2-RCaMP1.07 mice (tg(RP23-370F21-RCaMP1.07)B3-3Mik/J) express the fluorescent Ca²⁺ indicator RCaMP1.07 in SMCs under the control of the Acta2 locus promoter/enhancer regions were obtained from Jackson Laboratories (Bar Harbor, MN, USA). To generate cell-specific expression in two distinct cell types (ICC and SMCs) Acta2-RCaMP1.07 mice were bred with Kit^Cre‐ERT2/GCaMP6f^fl/fl mice. The offspring Kit-iCre-GCaMP6f/Acta2-RCaMP1.07 mice were identified by genotyping after receiving tamoxifen which served to delete the STOP cassette in the Cre-expressing cells; resulting in the expression of the fluorescent Ca²⁺ indicator protein, GCaMP6f. These mice allowed simultaneous, dual color imaging of ICC and SMCs. iCre mice were injected with tamoxifen (TAM; Intraperitoneal injection; IP) at 6–8 weeks of age (2 mg of TAM for three consecutive days), as described previously (Baker et al., 2016), to induce activation of the Cre recombinase and expression of optogenetic sensors. Mice were used for experiments 10–15 days after the tamoxifen injections. On days of experiments the mice were anaesthetized by inhalation of isoflurane (Baxter, Deerfield, IL, USA) and killed by cervical dislocation before excision of tissues.

The animals used, protocols performed and procedures in this study were in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by the Institutional Animal Use and Care Committee at the University of Nevada, Reno (IACUC; Protocol: 00053).

Tissue preparation

Colonic segments (2 cm in length, proximal region) were removed from mice after an abdominal incision and placed in Krebs-Ringer bicarbonate solution (KRB). The tissues were cut along the mesenteric border and intraluminal contents were washed away with KRB. Tissues were prepared by blunt dissection in two ways: (1) The submucosa layer was isolated after carefully removing the mucosal layer and the tunica muscularis. (2) The submucosa layer was left attached to the tunica muscularis after removal of the mucosa. The isolated submucosal layer preparation provided better imaging of ICC-SM by eliminating motion artifacts associated with muscle contractions. We used the isolated submucosal layer preparation in most cases in this study with the exception of two experiments where muscle attachments were necessary to test important questions (see Results).

Immunohistochemistry

Colonic tissues from wild-type mice were processed to assess distribution of c-Kit immunoreactivity. Whole mounts of submucosal layer after removing the mucosa and tunica muscularis were fixed in 4% paraformaldehyde and visualized as described previously (Sanders et al., 2014b). Briefly, after block with 1% bovine serum albumin, colonic tissues were incubated with a polyclonal antibody raised against c-Kit (mSCFR, R and D Systems, MN, USA; 1:500 dilution in 0.5% Triton-X working solution) for 48 hr. Immunoreactivity was detected using Alexa-488 labeled donkey anti-goat IgG (1:1000 in PBS; Invitrogen, NY, USA). Colonic tissues were visualized using a Zeiss LSM 510 confocal microscope and images were constructed using Image J software (National Institutes of Health, MD, USA, http://rsbweb.nih.gov/ij). ICC (copKit) images were visualized using a spinning-disk confocal system (CSU-W1; spinning disk, Yokogawa Electric, Tokyo, Japan).

Cell sorting and quantitative PCR

Kit^+/copGFP mice (B6.129S7-^{Kittm1Rosay/J}; 5–8 wks old) were used for evaluations of gene expression in ICC-SM. Cell-specific expression of the fluorescent reporter allows unequivocal identification of ICC (Ro et al., 2010). After cell dispersion, ICC-SM were sorted by fluorescence-activated cell sorting (FACS) and evaluated for purity as previously described (Baker et al., 2016). Total RNA was isolated using an Illustra RNAspin Mini RNA Isolation Kit (GE Healthcare). qScript cDNA SuperMix (Quanta Biosciences), used according to the manufacturer’s instructions, was used to synthesize first-strand cDNA. Quantitative PCR (qPCR) was performed using Fast Sybr Green chemistry on the 7900HT Fast Real-Time PCR System (Applied Biosystems) and gene-specific primers (Supplementary file 1). Regression analysis was performed to generate standard curves from the mean values of technical triplicate qPCRs of log10 diluted cDNA samples. Evaluation of gene expression in ICC-SM was compared with expression in the unsorted cells from the submucosal tissue of Kit^+/copGFP mice.

Ca²⁺ imaging

The isolated/intact submucosal layers were pinned to Sylgard coated dish and perfused with KRB solution at 37°C for a 60-min equilibration period. Ca²⁺ imaging was performed using a spinning-disk confocal system (CSU-W1; spinning disk, Yokogawa Electric, Tokyo, Japan) mounted on an upright Nikon Eclipse FN1 microscope equipped with several water immersion Nikon CFI Fluor lenses (10 × 0.3 NA, 20 × 0.5 NA, 40 × 0.8 NA, 60 × 0.8 NA and 100 × 1.1 NA) (Nikon Instruments, New York, USA). The system is equipped with two solid-state laser lines of 488 nm and 561 nm. The laser lines are combined with a borealis system (ANDOR Technology, Belfast, UK) to increase laser intensity and uniformity throughout the imaging FOV. The system also has two high-speed electron multiplying charged coupled devices (EMCCD) cameras (Andor iXon-Ultra 897 EMCCD Cameras; ANDOR Technology, Belfast, UK) to allow dual-color imaging simultaneously and maintain sensitive and fast speed acquisition at full frame of 512 × 512 active pixels as previously described (Baker et al., 2015). Briefly, images were captured, and image sequences were collected at 33 to 50 fps using MetaMorph software (MetaMorph Inc, TN, USA). In experiments with pharmacological agents, a control activity period of (30 s) was recorded prior of drug application into the chamber for 15 min.

Ca²⁺ imaging analysis

Movies of Ca²⁺ transients in ICC-SM were imported, preprocessed and analyzed using a combination of three image analysis programs: (1) custom build software (Volumetry G8d, Dr. Grant Hennig); (2) Fiji/Image J (National Institutes of Health, MD, USA, http://rsbweb.nih.gov/ij); (3) Automated Spatio Temporal Map analysis plugin (STMapAuto), https://github.com/gdelvalle99/STMapAuto as described previously (Drumm et al., 2017; Drumm et al., 2019b; Leigh et al., 2020). Briefly, movies of Ca²⁺ transients (stacks of TIFF images) were imported into Volumetry G8d and motion stabilized, background subtracted and smoothed (Gaussian filter: 1.5 × 1.5 µm, StdDev 1.0). A particle analysis routine was employed using a flood-fill algorithm to enhance Ca²⁺ transient detection. particles (PTCLs) representing the areas of active Ca²⁺ signals in cells were saved as a coordinate-based particle movie and combined area and total number of PTCLs were calculated. To better isolate firing sites, only those particles that did not overlap with any particles in the previous frame but overlap with particles in the subsequent 70 ms were considered firing/initiation sites. To show the overall regions in cells where Ca²⁺ transients occurred, PTCLs were summed throughout the video to create an image of their occurrence. The spatial information in the Ca²⁺ occurrence maps data is an indication of each firing site that gives information on their temporal activation and provides no information on their spatial spread. This was implemented in our analysis to accommodate a large number of firing sites and effectively plot them in a 2D occurrence map.

Drugs and solutions

All tissues were perfused continuously with KRB solution containing (mmol/L): NaCl, 5.9; NaHCO₃, 120.35; KCl, 1.2; MgCl₂, 15.5; NaH₂PO₄,1.2; CaCl₂, 2.5; and glucose, 11.5. The KRB solution was warmed to a physiological temperature of 37 ± 0.3°C and bubbled with a mixture of 97% O₂ – 3% CO₂. For experiments utilizing external solutions with 0 [Ca²⁺]_o, CaCl₂ was omitted and 0.5 mM ethylene glycol-bis (β-aminoethyl ether)-N, N, N’, N’–tetraacetic acid (EGTA) was added to the solution. NNC 55–0396 and TTA-A2 were purchased from Alomone Labs (Jerusalem, Israel). 2-aminoethyl-diphenylborinate (2-APB), tetracaine, nicardipine, pinacidil was purchased from Millipore-Sigma (St. Louis, Missouri, USA). Thapsigargin, isradipine, Z-944, CPA and ryanodine were purchased from Tocris Bioscience (Ellisville, Missouri, USA). GSK 7975A was purchased from Aobious (Aobious INC, MA, USA), and xestospongin C (XeC) was purchased from Cayman Chemical (Michigan, USA).

Statistical analysis

Data is presented as the mean ± standard error unless otherwise stated. Statistical analysis was performed using either a Students t-test or one-way ANOVA with a Tukey post hoc test where appropriate. In all tests, p<0.05 was considered significant. When describing data, n refers to the number of animals used in a dataset. Probabilities < 0.05 are represented by a single asterisk (*), probabilities < 0.01 are represented by two asterisks (**), probabilities < 0.001 are represented by three asterisks (***) and probabilities < 0.0001 are represented by four asterisks (****). All statistical tests were performed using GraphPad Prism 8.0.1 (San Diego, CA).

Acknowledgements

The authors extend their sincere appreciation to Yulia Bayguinov for assistance with immunohistochemical experiments, Lauren O’Kane for assistance with qPCR experiments and David White and Emily Fox for assistance with FACS.

Funding Statement

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Contributor Information

Salah A Baker, Email: sabubaker@med.unr.edu.

Mark T Nelson, University of Vermont, United States.

Richard W Aldrich, The University of Texas at Austin, United States.

Funding Information

This paper was supported by the following grants:

National Institute of Diabetes and Digestive and Kidney Diseases R01 DK-120759 to Salah A Baker, Kenton M Sanders.
National Institute of Diabetes and Digestive and Kidney Diseases R01 DK-078736 to Caroline A Cobine.

Additional information

Competing interests

No competing interests declared.

Author contributions

Conceptualization, Resources, Data curation, Software, Formal analysis, Supervision, Funding acquisition, Validation, Investigation, Visualization, Methodology, Writing - original draft, Project administration, Writing - review and editing.

Data curation, Formal analysis, Investigation, Methodology.

Formal analysis.

Data curation, Formal analysis, Investigation.

Validation, Writing - review and editing.

Data curation, Formal analysis, Methodology, Writing - review and editing.

Formal analysis, Investigation, Methodology, Writing - review and editing.

Conceptualization, Resources, Data curation, Supervision, Funding acquisition, Validation, Writing - original draft, Project administration, Writing - review and editing.

Ethics

Animal experimentation: The animals used, protocols performed and procedures in this study were in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by the Institutional Animal Use and Care Committee at the University of Nevada, Reno (IACUC; Protocol# 00053).

Additional files

Supplementary file 1. Table summary of gene primers sequences of Kit, Ano1, Myh11, Uchl1, Cacna1c, Cacna1d, Cacna1g, Cacna1h, and Orai1-3.

elife-64099-supp1.docx^{(14.3KB, docx)}

Transparent reporting form

elife-64099-transrepform.docx^{(246.3KB, docx)}

Data availability

All data generated or analysed during this study are included in the manuscript and supporting files.

References

Amberg GC, Navedo MF, Santana LF. On the loose: uncaging Ca2+-induced Ca2+ release in smooth muscle. Journal of General Physiology. 2006;127:221–223. doi: 10.1085/jgp.200609510. [DOI] [PMC free article] [PubMed] [Google Scholar]
Anekonda TS, Quinn JF, Harris C, Frahler K, Wadsworth TL, Woltjer RL. L-type voltage-gated calcium channel blockade with isradipine as a therapeutic strategy for alzheimer's disease. Neurobiology of Disease. 2011;41:62–70. doi: 10.1016/j.nbd.2010.08.020. [DOI] [PMC free article] [PubMed] [Google Scholar]
Baker SA, Hennig GW, Ward SM, Sanders KM. Temporal sequence of activation of cells involved in Purinergic neurotransmission in the Colon. The Journal of Physiology. 2015;593:1945–1963. doi: 10.1113/jphysiol.2014.287599. [DOI] [PMC free article] [PubMed] [Google Scholar]
Baker SA, Drumm BT, Saur D, Hennig GW, Ward SM, Sanders KM. Spontaneous ca(2+) transients in interstitial cells of cajal located within the deep muscular plexus of the murine small intestine. The Journal of Physiology. 2016;594:3317–3338. doi: 10.1113/JP271699. [DOI] [PMC free article] [PubMed] [Google Scholar]
Bayguinov O, Ward SM, Kenyon JL, Sanders KM. Voltage-gated Ca2+ currents are necessary for slow-wave propagation in the canine gastric antrum. American Journal of Physiology. Cell Physiology. 2007;293:C1645–C1659. doi: 10.1152/ajpcell.00165.2007. [DOI] [PubMed] [Google Scholar]
Beckett EA, Bayguinov YR, Sanders KM, Ward SM, Hirst GD. Properties of unitary potentials generated by intramuscular interstitial cells of cajal in the murine and guinea-pig gastric fundus. The Journal of Physiology. 2004;559:259–269. doi: 10.1113/jphysiol.2004.063016. [DOI] [PMC free article] [PubMed] [Google Scholar]
Bell DC, Butcher AJ, Berrow NS, Page KM, Brust PF, Nesterova A, Stauderman KA, Seabrook GR, Nürnberg B, Dolphin AC. Biophysical properties, pharmacology, and modulation of human, neuronal L-type (alpha(1D), Ca(V)1.3) voltage-dependent calcium currents. Journal of Neurophysiology. 2001;85:816–827. doi: 10.1152/jn.2001.85.2.816. [DOI] [PubMed] [Google Scholar]
Berezin I, Huizinga JD, Daniel EE. Interstitial cells of cajal in the canine Colon: a special communication network at the inner border of the circular muscle. The Journal of Comparative Neurology. 1988;273:42–51. doi: 10.1002/cne.902730105. [DOI] [PubMed] [Google Scholar]
Berjukow S, Marksteiner R, Gapp F, Sinnegger MJ, Hering S. Molecular mechanism of calcium channel block by isradipine role of a drug-induced inactivated channel conformation. The Journal of Biological Chemistry. 2000;275:22114–22120. doi: 10.1074/jbc.M908836199. [DOI] [PubMed] [Google Scholar]
Berridge MJ. Inositol trisphosphate and calcium signalling mechanisms. Biochimica Et Biophysica Acta (BBA) - Molecular Cell Research. 2009;1793:933–940. doi: 10.1016/j.bbamcr.2008.10.005. [DOI] [PubMed] [Google Scholar]
Burns AJ, Lomax AE, Torihashi S, Sanders KM, Ward SM. Interstitial cells of cajal mediate inhibitory neurotransmission in the stomach. PNAS. 1996;93:12008–12013. doi: 10.1073/pnas.93.21.12008. [DOI] [PMC free article] [PubMed] [Google Scholar]
Burns AJ, Herbert TM, Ward SM, Sanders KM. Interstitial cells of cajal in the guinea-pig gastrointestinal tract as revealed by c-Kit immunohistochemistry. Cell and Tissue Research. 1997;290:11–20. doi: 10.1007/s004410050902. [DOI] [PubMed] [Google Scholar]
Butorac C, Krizova A, Derler I. Review: structure and activation mechanisms of CRAC channels. Advances in Experimental Medicine and Biology. 2020;1131:547–604. doi: 10.1007/978-3-030-12457-1_23. [DOI] [PubMed] [Google Scholar]
Chauvet S, Jarvis L, Chevallet M, Shrestha N, Groschner K, Bouron A. Pharmacological characterization of the native Store-Operated calcium channels of cortical neurons from embryonic mouse brain. Frontiers in Pharmacology. 2016;7:486. doi: 10.3389/fphar.2016.00486. [DOI] [PMC free article] [PubMed] [Google Scholar]
Chen J, Sanderson MJ. Store-operated calcium entry is required for sustained contraction and Ca2+ oscillations of airway smooth muscle. The Journal of Physiology. 2017;595:3203–3218. doi: 10.1113/JP272694. [DOI] [PMC free article] [PubMed] [Google Scholar]
Corsetti M, Costa M, Bassotti G, Bharucha AE, Borrelli O, Dinning P, Di Lorenzo C, Huizinga JD, Jimenez M, Rao S, Spiller R, Spencer NJ, Lentle R, Pannemans J, Thys A, Benninga M, Tack J. First translational consensus on terminology and definitions of colonic motility in animals and humans studied by manometric and other techniques. Nature Reviews Gastroenterology & Hepatology. 2019;16:559–579. doi: 10.1038/s41575-019-0167-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
Cousins HM, Edwards FR, Hickey H, Hill CE, Hirst GD. Electrical coupling between the myenteric interstitial cells of cajal and adjacent muscle layers in the guinea-pig gastric antrum. The Journal of Physiology. 2003;550:829–844. doi: 10.1113/jphysiol.2003.042176. [DOI] [PMC free article] [PubMed] [Google Scholar]
D'Antona G, Hennig GW, Costa M, Humphreys CM, Brookes SJ. Analysis of motor patterns in the isolated guinea-pig large intestine by spatio-temporal maps. Neurogastroenterology and Motility. 2001;13:483–492. doi: 10.1046/j.1365-2982.2001.00282.x. [DOI] [PubMed] [Google Scholar]
Derler I, Schindl R, Fritsch R, Heftberger P, Riedl MC, Begg M, House D, Romanin C. The action of selective CRAC channel blockers is affected by the orai pore geometry. Cell Calcium. 2013;53:139–151. doi: 10.1016/j.ceca.2012.11.005. [DOI] [PMC free article] [PubMed] [Google Scholar]
Drumm BT, Hennig GW, Battersby MJ, Cunningham EK, Sung TS, Ward SM, Sanders KM, Baker SA. Clustering of Ca2+ transients in interstitial cells of cajal defines slow wave duration. Journal of General Physiology. 2017;149:703–725. doi: 10.1085/jgp.201711771. [DOI] [PMC free article] [PubMed] [Google Scholar]
Drumm BT, Hwang SJ, Baker SA, Ward SM, Sanders KM. Ca2+ signalling behaviours of intramuscular interstitial cells of cajal in the murine Colon. The Journal of Physiology. 2019a;597:3587–3617. doi: 10.1113/JP278036. [DOI] [PMC free article] [PubMed] [Google Scholar]
Drumm BT, Hennig GW, Baker SA, Sanders KM. Applications of Spatio-temporal mapping and particle analysis techniques to quantify intracellular Ca2+ signaling in situ. Journal of Visualized Experiments. 2019b;7:58989. doi: 10.3791/58989. [DOI] [PMC free article] [PubMed] [Google Scholar]
Drumm BT, Rembetski BE, Messersmith K, Manierka MS, Baker SA, Sanders KM. Pacemaker function and neural responsiveness of subserosal interstitial cells of cajal in the mouse Colon. The Journal of Physiology. 2020a;598:651–681. doi: 10.1113/JP279102. [DOI] [PMC free article] [PubMed] [Google Scholar]
Drumm BT, Rembetski BE, Huynh K, Nizar A, Baker SA, Sanders KM. Excitatory cholinergic responses in mouse Colon intramuscular interstitial cells of cajal are due to enhanced ca. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. 2020b;34:10073–10095. doi: 10.1096/fj.202000672R. [DOI] [PMC free article] [PubMed] [Google Scholar]
Dupont G, Goldbeter A. Properties of intracellular Ca2+ waves generated by a model based on ca(2+)-induced Ca2+ release. Biophysical Journal. 1994;67:2191–2204. doi: 10.1016/S0006-3495(94)80705-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
Durdle NG, Kingma YJ, Bowes KL, Chambers MM. Origin of slow waves in the canine Colon. Gastroenterology. 1983;84:375–382. doi: 10.1016/S0016-5085(83)80136-X. [DOI] [PubMed] [Google Scholar]
Faussone Pellegrini MS, Cortesini C, Romagnoli P. Ultrastructure of the Tunica muscularis of the cardial portion of the human esophagus and stomach, with special reference to the so-called cajal's interstitial cells] Archivio Italiano Di Anatomia E Di Embriologia. Italian Journal of Anatomy and Embryology. 1977;82:157–177. [PubMed] [Google Scholar]
Faussone-Pellegrini MS. Histogenesis, structure and relationships of interstitial cells of cajal (ICC): from morphology to functional interpretation. European Journal of Morphology. 1992;30:137–148. [PubMed] [Google Scholar]
Fill M, Copello JA. Ryanodine receptor calcium release channels. Physiological Reviews. 2002;82:893–922. doi: 10.1152/physrev.00013.2002. [DOI] [PubMed] [Google Scholar]
Gallego D, Gil V, Aleu J, Aulí M, Clavé P, Jiménez M. Purinergic and nitrergic junction potential in the human Colon. American Journal of Physiology-Gastrointestinal and Liver Physiology. 2008;295:G522–G533. doi: 10.1152/ajpgi.00510.2007. [DOI] [PubMed] [Google Scholar]
Gibson A, McFadzean I, Wallace P, Wayman CP. Capacitative Ca2+ entry and the regulation of smooth muscle tone. Trends in Pharmacological Sciences. 1998;19:266–269. doi: 10.1016/s0165-6147(98)01222-x. [DOI] [PubMed] [Google Scholar]
Gomez-Pinilla PJ, Gibbons SJ, Bardsley MR, Lorincz A, Pozo MJ, Pasricha PJ, Van de Rijn M, West RB, Sarr MG, Kendrick ML, Cima RR, Dozois EJ, Larson DW, Ordog T, Farrugia G. Ano1 is a selective marker of interstitial cells of cajal in the human and mouse gastrointestinal tract. American Journal of Physiology-Gastrointestinal and Liver Physiology. 2009;296:G1370–G1381. doi: 10.1152/ajpgi.00074.2009. [DOI] [PMC free article] [PubMed] [Google Scholar]
Hara Y, Kubota M, Szurszewski JH. Electrophysiology of smooth muscle of the small intestine of some mammals. The Journal of Physiology. 1986;372:501–520. doi: 10.1113/jphysiol.1986.sp016022. [DOI] [PMC free article] [PubMed] [Google Scholar]
Hennig GW, Hirst GD, Park KJ, Smith CB, Sanders KM, Ward SM, Smith TK. Propagation of pacemaker activity in the guinea-pig antrum. The Journal of Physiology. 2004;556:585–599. doi: 10.1113/jphysiol.2003.059055. [DOI] [PMC free article] [PubMed] [Google Scholar]
Hirano Y, Fozzard HA, January CT. Characteristics of L- and T-type Ca2+ currents in canine cardiac purkinje cells. American Journal of Physiology-Heart and Circulatory Physiology. 1989;256:H1478–H1492. doi: 10.1152/ajpheart.1989.256.5.H1478. [DOI] [PubMed] [Google Scholar]
Hotta A, Okada N, Suzuki H. Mibefradil-sensitive component involved in the plateau potential in submucosal interstitial cells of the murine proximal Colon. Biochemical and Biophysical Research Communications. 2007;353:170–176. doi: 10.1016/j.bbrc.2006.12.003. [DOI] [PubMed] [Google Scholar]
Huang X, Lee SH, Lu H, Sanders KM, Koh SD. Molecular and functional characterization of inwardly rectifying K + currents in murine proximal Colon. The Journal of Physiology. 2018;596:379–391. doi: 10.1113/JP275234. [DOI] [PMC free article] [PubMed] [Google Scholar]
Huizinga JD, Shin A, Chow E. Electrical coupling and pacemaker activity in colonic smooth muscle. American Journal of Physiology-Cell Physiology. 1988;255:C653–C660. doi: 10.1152/ajpcell.1988.255.5.C653. [DOI] [PubMed] [Google Scholar]
Huizinga JD, Thuneberg L, Klüppel M, Malysz J, Mikkelsen HB, Bernstein A. W/kit gene required for interstitial cells of cajal and for intestinal pacemaker activity. Nature. 1995;373:347–349. doi: 10.1038/373347a0. [DOI] [PubMed] [Google Scholar]
Hwang SJ, Blair PJ, Britton FC, O'Driscoll KE, Hennig G, Bayguinov YR, Rock JR, Harfe BD, Sanders KM, Ward SM. Expression of anoctamin 1/TMEM16A by interstitial cells of cajal is fundamental for slow wave activity in gastrointestinal muscles. The Journal of Physiology. 2009;587:4887–4904. doi: 10.1113/jphysiol.2009.176198. [DOI] [PMC free article] [PubMed] [Google Scholar]
Hwang SJ, Blair PJ, Durnin L, Mutafova-Yambolieva V, Sanders KM, Ward SM. P2Y1 purinoreceptors are fundamental to inhibitory motor control of murine colonic excitability and transit. The Journal of Physiology. 2012;590:1957–1972. doi: 10.1113/jphysiol.2011.224634. [DOI] [PMC free article] [PubMed] [Google Scholar]
Ishikawa K, Komuro T. Characterization of the interstitial cells associated with the submuscular plexus of the guinea-pig Colon. Anatomy and Embryology. 1996;194:49–55. doi: 10.1007/BF00196314. [DOI] [PubMed] [Google Scholar]
Kaßmann M, Szijártó IA, García-Prieto CF, Fan G, Schleifenbaum J, Anistan YM, Tabeling C, Shi Y, le Noble F, Witzenrath M, Huang Y, Markó L, Nelson MT, Gollasch M. Role of ryanodine type 2 receptors in elementary ca. Journal of the American Heart Association. 2019;8:e010090. doi: 10.1161/JAHA.118.010090. [DOI] [PMC free article] [PubMed] [Google Scholar]
Keef KD, Ward SM, Stevens RJ, Frey BW, Sanders KM. Electrical and mechanical effects of acetylcholine and substance P in subregions of canine Colon. American Journal of Physiology-Gastrointestinal and Liver Physiology. 1992;262:G298–G307. doi: 10.1152/ajpgi.1992.262.2.G298. [DOI] [PubMed] [Google Scholar]
Keef KD, Anderson U, O'Driscoll K, Ward SM, Sanders KM. Electrical activity induced by nitric oxide in canine colonic circular muscle. American Journal of Physiology-Gastrointestinal and Liver Physiology. 2002;282:G123–G129. doi: 10.1152/ajpgi.00217.2001. [DOI] [PubMed] [Google Scholar]
Kito Y, Ward SM, Sanders KM. Pacemaker potentials generated by interstitial cells of cajal in the murine intestine. American Journal of Physiology-Cell Physiology. 2005;288:C710–C720. doi: 10.1152/ajpcell.00361.2004. [DOI] [PubMed] [Google Scholar]
Kito Y, Suzuki H. Modulation of slow waves by hyperpolarization with potassium channel openers in antral smooth muscle of the guinea-pig stomach. The Journal of Physiology. 2003;548:175–189. doi: 10.1113/jphysiol.2002.035550. [DOI] [PMC free article] [PubMed] [Google Scholar]
Koh SD, Bradley KK, Rae MG, Keef KD, Horowitz B, Sanders KM. Basal activation of ATP-sensitive potassium channels in murine colonic smooth muscle cell. Biophysical Journal. 1998;75:1793–1800. doi: 10.1016/S0006-3495(98)77621-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
Komuro T, Tokui K, Zhou DS. Identification of the interstitial cells of cajal. Histology and Histopathology. 1996;11:769–786. [PubMed] [Google Scholar]
Komuro T. Comparative morphology of interstitial cells of cajal: ultrastructural characterization. Microscopy Research and Technique. 1999;47:267–285. doi: 10.1002/(SICI)1097-0029(19991115)47:4<267::AID-JEMT5>3.0.CO;2-O. [DOI] [PubMed] [Google Scholar]
Komuro T. Structure and organization of interstitial cells of cajal in the gastrointestinal tract. The Journal of Physiology. 2006;576:653–658. doi: 10.1113/jphysiol.2006.116624. [DOI] [PMC free article] [PubMed] [Google Scholar]
Koschak A, Reimer D, Huber I, Grabner M, Glossmann H, Engel J, Striessnig J. alpha 1d (Cav1.3) Subunits Can Form L-type Ca 2+Channels Activating at Negative Voltages. Journal of Biological Chemistry. 2001;276:22100–22106. doi: 10.1074/jbc.M101469200. [DOI] [PubMed] [Google Scholar]
Langton P, Ward SM, Carl A, Norell MA, Sanders KM. Spontaneous electrical activity of interstitial cells of cajal isolated from canine proximal Colon. PNAS. 1989;86:7280–7284. doi: 10.1073/pnas.86.18.7280. [DOI] [PMC free article] [PubMed] [Google Scholar]
Lee MY, Ha SE, Park C, Park PJ, Fuchs R, Wei L, Jorgensen BG, Redelman D, Ward SM, Sanders KM, Ro S. Transcriptome of interstitial cells of cajal reveals unique and selective gene signatures. PLOS ONE. 2017;12:e0176031. doi: 10.1371/journal.pone.0176031. [DOI] [PMC free article] [PubMed] [Google Scholar]
Leigh WA, Del Valle G, Kamran SA, Drumm BT, Tavakkoli A, Sanders KM, Baker SA. A high throughput machine-learning driven analysis of ca. Cell Calcium. 2020;91:102260. doi: 10.1016/j.ceca.2020.102260. [DOI] [PMC free article] [PubMed] [Google Scholar]
Lies B, Gil V, Groneberg D, Seidler B, Saur D, Wischmeyer E, Jiménez M, Friebe A. Interstitial cells of cajal mediate nitrergic inhibitory neurotransmission in the murine gastrointestinal tract. American Journal of Physiology-Gastrointestinal and Liver Physiology. 2014;307:G98–G106. doi: 10.1152/ajpgi.00082.2014. [DOI] [PubMed] [Google Scholar]
Lyfenko AD, Dirksen RT. Differential dependence of store-operated and excitation-coupled Ca2+ entry in skeletal muscle on STIM1 and Orai1. The Journal of Physiology. 2008;586:4815–4824. doi: 10.1113/jphysiol.2008.160481. [DOI] [PMC free article] [PubMed] [Google Scholar]
Navedo MF, Amberg GC. Local regulation of L-type Ca²⁺ channel sparklets in arterial smooth muscle. Microcirculation. 2013a;20:290–298. doi: 10.1111/micc.12021. [DOI] [PMC free article] [PubMed] [Google Scholar]
Navedo MF, Santana LF. CaV1.2 sparklets in heart and vascular smooth muscle. Journal of Molecular and Cellular Cardiology. 2013b;58:67–76. doi: 10.1016/j.yjmcc.2012.11.018. [DOI] [PMC free article] [PubMed] [Google Scholar]
Nowycky MC, Fox AP, Tsien RW. Three types of neuronal calcium channel with different calcium agonist sensitivity. Nature. 1985;316:440–443. doi: 10.1038/316440a0. [DOI] [PubMed] [Google Scholar]
Park KJ, Hennig GW, Lee HT, Spencer NJ, Ward SM, Smith TK, Sanders KM. Spatial and temporal mapping of pacemaker activity in interstitial cells of cajal in mouse ileum in situ. American Journal of Physiology-Cell Physiology. 2006;290:C1411–C1427. doi: 10.1152/ajpcell.00447.2005. [DOI] [PubMed] [Google Scholar]
Plujà L, Albertí E, Fernández E, Mikkelsen HB, Thuneberg L, Jiménez M. Evidence supporting presence of two pacemakers in rat Colon. American Journal of Physiology-Gastrointestinal and Liver Physiology. 2001;281:G255–G266. doi: 10.1152/ajpgi.2001.281.1.G255. [DOI] [PubMed] [Google Scholar]
Prakriya M, Lewis RS. Store-Operated calcium channels. Physiological Reviews. 2015;95:1383–1436. doi: 10.1152/physrev.00020.2014. [DOI] [PMC free article] [PubMed] [Google Scholar]
Putney JW. The physiological function of store-operated calcium entry. Neurochemical Research. 2011;36:1157–1165. doi: 10.1007/s11064-010-0383-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
Putney JW. Forms and functions of store-operated calcium entry mediators, STIM and orai. Advances in Biological Regulation. 2018;68:88–96. doi: 10.1016/j.jbior.2017.11.006. [DOI] [PMC free article] [PubMed] [Google Scholar]
Ro S, Park C, Jin J, Zheng H, Blair PJ, Redelman D, Ward SM, Yan W, Sanders KM. A model to study the phenotypic changes of interstitial cells of cajal in gastrointestinal diseases. Gastroenterology. 2010;138:1068–1078. doi: 10.1053/j.gastro.2009.11.007. [DOI] [PMC free article] [PubMed] [Google Scholar]
Rosli RM, Heitmann PT, Kumar R, Hibberd TJ, Costa M, Wiklendt L, Wattchow DA, Arkwright J, de Fontgalland D, Brookes SJH, Spencer NJ, Dinning PG. Distinct patterns of myogenic motor activity identified in isolated human distal Colon with high-resolution manometry. Neurogastroenterology & Motility. 2020;32:e13871. doi: 10.1111/nmo.13871. [DOI] [PMC free article] [PubMed] [Google Scholar]
Rüegg UT, Hof RP. Pharmacology of the calcium antagonist isradipine. Drugs. 1990;40:3–9. doi: 10.2165/00003495-199000402-00004. [DOI] [PubMed] [Google Scholar]
Rumessen JJ, Vanderwinden JM, Hansen A, Horn T. Ultrastructure of interstitial cells in subserosa of human Colon. Cells Tissues Organs. 2013;197:322–332. doi: 10.1159/000346314. [DOI] [PubMed] [Google Scholar]
Rumessen JJ, Thuneberg L. Plexus muscularis profundus and associated interstitial cells. I. light microscopical studies of mouse small intestine. The Anatomical Record. 1982;203:115–127. doi: 10.1002/ar.1092030111. [DOI] [PubMed] [Google Scholar]
Sanders KM, Stevens R, Burke E, Ward SW. Slow waves actively propagate at Submucosal surface of circular layer in canine Colon. American Journal of Physiology-Gastrointestinal and Liver Physiology. 1990;259:G258–G263. doi: 10.1152/ajpgi.1990.259.2.G258. [DOI] [PubMed] [Google Scholar]
Sanders KM, Hwang SJ, Ward SM. Neuroeffector apparatus in gastrointestinal smooth muscle organs. The Journal of Physiology. 2010;588:4621–4639. doi: 10.1113/jphysiol.2010.196030. [DOI] [PMC free article] [PubMed] [Google Scholar]
Sanders KM, Koh SD, Ro S, Ward SM. Regulation of gastrointestinal motility--insights from smooth muscle biology. Nature Reviews Gastroenterology & Hepatology. 2012;9:633–645. doi: 10.1038/nrgastro.2012.168. [DOI] [PMC free article] [PubMed] [Google Scholar]
Sanders KM, Ward SM, Koh SD. Interstitial cells: regulators of smooth muscle function. Physiological Reviews. 2014a;94:859–907. doi: 10.1152/physrev.00037.2013. [DOI] [PMC free article] [PubMed] [Google Scholar]
Sanders KM, Salter AK, Hennig GW, Koh SD, Perrino BA, Ward SM, Baker SA. Responses to enteric motor neurons in the gastric fundus of mice with reduced intramuscular interstitial cells of cajal. Journal of Neurogastroenterology and Motility. 2014b;20:171–184. doi: 10.5056/jnm.2014.20.2.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
Santana LF, Navedo MF, Amberg GC, Nieves-Cintrón M, Votaw VS, Ufret-Vincenty CA. Calcium sparklets in arterial smooth muscle. Clinical and Experimental Pharmacology and Physiology. 2008;35:1121–1126. doi: 10.1111/j.1440-1681.2007.04867.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
Scholze A, Plant TD, Dolphin AC, Nürnberg B. Functional expression and characterization of a voltage-gated CaV1.3 (alpha1D) calcium channel subunit from an insulin-secreting cell line. Molecular Endocrinology. 2001;15:1211–1221. doi: 10.1210/mend.15.7.0666. [DOI] [PubMed] [Google Scholar]
Serio R, Barajas-Lopez C, Daniel EE, Berezin I, Huizinga JD. Slow-wave activity in Colon: role of network of submucosal interstitial cells of cajal. American Journal of Physiology-Gastrointestinal and Liver Physiology. 1991;260:G636–G645. doi: 10.1152/ajpgi.1991.260.4.G636. [DOI] [PubMed] [Google Scholar]
Smith TK, Reed JB, Sanders KM. Origin and propagation of electrical slow waves in circular muscle of canine proximal Colon. American Journal of Physiology-Cell Physiology. 1987a;252:C215–C224. doi: 10.1152/ajpcell.1987.252.2.C215. [DOI] [PubMed] [Google Scholar]
Smith TK, Reed JB, Sanders KM. Interaction of two electrical pacemakers in muscularis of canine proximal Colon. American Journal of Physiology-Cell Physiology. 1987b;252:C290–C299. doi: 10.1152/ajpcell.1987.252.3.C290. [DOI] [PubMed] [Google Scholar]
Sonkusare SK, Bonev AD, Ledoux J, Liedtke W, Kotlikoff MI, Heppner TJ, Hill-Eubanks DC, Nelson MT. Elementary Ca2+ signals through endothelial TRPV4 channels regulate vascular function. Science. 2012;336:597–601. doi: 10.1126/science.1216283. [DOI] [PMC free article] [PubMed] [Google Scholar]
Takeda Y, Nystoriak MA, Nieves-Cintrón M, Santana LF, Navedo MF. Relationship between Ca2+ sparklets and sarcoplasmic reticulum Ca2+ load and release in rat cerebral arterial smooth muscle. American Journal of Physiology. Heart and Circulatory Physiology. 2011;301:H2285–H2294. doi: 10.1152/ajpheart.00488.2011. [DOI] [PMC free article] [PubMed] [Google Scholar]
Thomas AP, Bird GS, Hajnóczky G, Robb-Gaspers LD, Putney JW. Spatial and temporal aspects of cellular calcium signaling. The FASEB Journal. 1996;10:1505–1517. doi: 10.1096/fasebj.10.13.8940296. [DOI] [PubMed] [Google Scholar]
Thuneberg L. Interstitial cells of cajal: intestinal pacemaker cells? Advances in Anatomy, Embryology, and Cell Biology. 1982;71:1–130. [PubMed] [Google Scholar]
Trebak M, Zhang W, Ruhle B, Henkel MM, González-Cobos JC, Motiani RK, Stolwijk JA, Newton RL, Zhang X. What role for store-operated Ca²⁺ entry in muscle? Microcirculation. 2013;20:330–336. doi: 10.1111/micc.12042. [DOI] [PMC free article] [PubMed] [Google Scholar]
Tseng GN, Boyden PA. Multiple types of Ca2+ currents in single canine purkinje cells. Circulation Research. 1989;65:1735–1750. doi: 10.1161/01.RES.65.6.1735. [DOI] [PubMed] [Google Scholar]
Vanderwinden JM, Rumessen JJ, Liu H, Descamps D, De Laet MH, Vanderhaeghen JJ. Interstitial cells of cajal in human Colon and in Hirschsprung's disease. Gastroenterology. 1996;111:901–910. doi: 10.1016/S0016-5085(96)70057-4. [DOI] [PubMed] [Google Scholar]
Vanderwinden JM, Rumessen JJ, Bernex F, Schiffmann SN, Panthier JJ. Distribution and ultrastructure of interstitial cells of cajal in the mouse Colon, using antibodies to kit and kit(W-lacZ) mice. Cell and Tissue Research. 2000;302:155–170. doi: 10.1007/s004419900170. [DOI] [PubMed] [Google Scholar]
Vierra NC, Kirmiz M, van der List D, Santana LF, Trimmer JS. Kv2.1 mediates spatial and functional coupling of L-type calcium channels and ryanodine receptors in mammalian neurons. eLife. 2019;8:e49953. doi: 10.7554/eLife.49953. [DOI] [PMC free article] [PubMed] [Google Scholar]
Ward SM, Keller RG, Sanders KM. Structure and organization of electrical activity of canine distal Colon. American Journal of Physiology-Gastrointestinal and Liver Physiology. 1991;260:G724–G735. doi: 10.1152/ajpgi.1991.260.5.G724. [DOI] [PubMed] [Google Scholar]
Ward SM, Burns AJ, Torihashi S, Sanders KM. Mutation of the proto-oncogene c-kit blocks development of interstitial cells and electrical rhythmicity in murine intestine. The Journal of Physiology. 1994;480:91–97. doi: 10.1113/jphysiol.1994.sp020343. [DOI] [PMC free article] [PubMed] [Google Scholar]
Ward SM, Burns AJ, Torihashi S, Harney SC, Sanders KM. Impaired development of interstitial cells and intestinal electrical rhythmicity in steel mutants. American Journal of Physiology-Cell Physiology. 1995;269:C1577–C1585. doi: 10.1152/ajpcell.1995.269.6.C1577. [DOI] [PubMed] [Google Scholar]
Ward SM, Beckett EA, Wang X, Baker F, Khoyi M, Sanders KM. Interstitial cells of cajal mediate cholinergic neurotransmission from enteric motor neurons. The Journal of Neuroscience. 2000a;20:1393–1403. doi: 10.1523/JNEUROSCI.20-04-01393.2000. [DOI] [PMC free article] [PubMed] [Google Scholar]
Ward SM, Ordog T, Koh SD, Baker SA, Jun JY, Amberg G, Monaghan K, Sanders KM. Pacemaking in interstitial cells of cajal depends upon calcium handling by endoplasmic reticulum and mitochondria. The Journal of Physiology. 2000b;525:355–361. doi: 10.1111/j.1469-7793.2000.t01-1-00355.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
Won KJ, Sanders KM, Ward SM. Interstitial cells of cajal mediate mechanosensitive responses in the stomach. PNAS. 2005;102:14913–14918. doi: 10.1073/pnas.0503628102. [DOI] [PMC free article] [PubMed] [Google Scholar]
Xu X, Best PM. Postnatal changes in T-type calcium current density in rat atrial myocytes. The Journal of Physiology. 1992;454:657–672. doi: 10.1113/jphysiol.1992.sp019285. [DOI] [PMC free article] [PubMed] [Google Scholar]
Xu W, Lipscombe D. Neuronal ca(V)1.3alpha(1) L-type channels activate at relatively hyperpolarized membrane potentials and are incompletely inhibited by dihydropyridines. The Journal of Neuroscience. 2001;21:5944–5951. doi: 10.1523/JNEUROSCI.21-16-05944.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
Yoneda S, Takano H, Takaki M, Suzuki H. Properties of spontaneously active cells distributed in the submucosal layer of mouse proximal Colon. The Journal of Physiology. 2002;542:887–897. doi: 10.1113/jphysiol.2002.018705. [DOI] [PMC free article] [PubMed] [Google Scholar]
Yoneda S, Takano H, Takaki M, Suzuki H. Effects of nifedipine and nickel on plateau potentials generated in submucosal interstitial cells distributed in the mouse proximal Colon. Journal of Smooth Muscle Research. 2003;39:55–65. doi: 10.1540/jsmr.39.55. [DOI] [PubMed] [Google Scholar]
Yoneda S, Fukui H, Takaki M. Pacemaker activity from submucosal interstitial cells of cajal drives high-frequency and low-amplitude circular muscle contractions in the mouse proximal Colon. Neurogastroenterology and Motility. 2004;16:621–627. doi: 10.1111/j.1365-2982.2004.00546.x. [DOI] [PubMed] [Google Scholar]
Youm JB, Zheng H, Koh SD, Sanders KM. Na-K-2Cl cotransporter and Store-Operated Ca2+ Entry in Pacemaking by Interstitial Cells of Cajal. Biophysical Journal. 2019;117:767–779. doi: 10.1016/j.bpj.2019.07.020. [DOI] [PMC free article] [PubMed] [Google Scholar]
Zheng H, Park KS, Koh SD, Sanders KM. Expression and function of a T-type Ca2+ conductance in interstitial cells of cajal of the murine small intestine. American Journal of Physiology-Cell Physiology. 2014;306:C705–C713. doi: 10.1152/ajpcell.00390.2013. [DOI] [PMC free article] [PubMed] [Google Scholar]
Zheng H, Drumm BT, Earley S, Sung TS, Koh SD, Sanders KM. SOCE mediated by STIM and orai is essential for pacemaker activity in the interstitial cells of cajal in the gastrointestinal tract. Science Signaling. 2018;11:eaaq0918. doi: 10.1126/scisignal.aaq0918. [DOI] [PMC free article] [PubMed] [Google Scholar]
Zhu MH, Kim TW, Ro S, Yan W, Ward SM, Koh SD, Sanders KM. A ca(2+)-activated cl(-) conductance in interstitial cells of cajal linked to slow wave currents and pacemaker activity. The Journal of Physiology. 2009;587:4905–4918. doi: 10.1113/jphysiol.2009.176206. [DOI] [PMC free article] [PubMed] [Google Scholar]
Zhu MH, Sung IK, Zheng H, Sung TS, Britton FC, O'Driscoll K, Koh SD, Sanders KM. Muscarinic activation of Ca2+-activated cl- current in interstitial cells of cajal. The Journal of Physiology. 2011;589:4565–4582. doi: 10.1113/jphysiol.2011.211094. [DOI] [PMC free article] [PubMed] [Google Scholar]
Zhu MH, Sung TS, O'Driscoll K, Koh SD, Sanders KM. Intracellular ca(2+) release from endoplasmic reticulum regulates slow wave currents and pacemaker activity of interstitial cells of cajal. American Journal of Physiology-Cell Physiology. 2015;308:C608–C620. doi: 10.1152/ajpcell.00360.2014. [DOI] [PMC free article] [PubMed] [Google Scholar]

eLife. doi: 10.7554/eLife.64099.sa1

Decision letter

Editor: Mark T Nelson¹

Reviewed by: Michael J Davis²

In the interests of transparency, eLife publishes the most substantive revision requests and the accompanying author responses.

Acceptance summary:

Gastrointestinal motility is controlled by the activity of interstitial cells of Cajal (ICC). Here the authors elucidate the calcium signals that drive the activity of submucosal ICCs which in turn elicit phasic contractions of the colon. The authors demonstrate that the calcium signals depend on calcium entry into submucosal ICCs.

Decision letter after peer review:

Thank you for submitting your article "Ca²⁺ signaling driving pacemaker activity in submucosal interstitial cells of Cajal in the colon" for consideration by eLife. Your article has been reviewed by three peer reviewers, one of whom is a member of our Board of Reviewing Editors, and the evaluation has been overseen by Richard Aldrich as the Senior Editor. The following individual involved in review of your submission has agreed to reveal their identity: Michael J Davis (Reviewer #3).

The reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission.

We would like to draw your attention to changes in our revision policy that we have made in response to COVID-19 (https://elifesciences.org/articles/57162). Specifically, we are asking editors to accept without delay manuscripts, like yours, that they judge can stand as eLife papers without additional data, even if they feel that they would make the manuscript stronger. Thus the revisions requested below only address clarity and presentation.

Summary:

In the manuscript entitled "Ca²⁺ signaling driving pacemaker activity in submucosal interstitial cells of Cajal in the colon", the authors investigated the role of different Ca²⁺ pathways on pacemaker (slow wave) activity in the mouse colon. While much of the investigation uses techniques and tools used to previously by the authors study Ca²⁺ dynamics in ICC in other regions of the gut (small intestine), they have refined the colonic tissue preparation to remove motion artifacts and have used dual Ca²⁺ indicator mice to better understand how ICC-SM influence smooth muscle Ca²⁺ dynamics and contraction. Overall, this solid study has used a comprehensive battery of drugs that interfere with Ca²⁺ signaling to demonstrate that extracellular Ca²⁺ entry is a particularly important component of pacemaker activity in ICC-SM.

Revisions:

1) Paradoxically, firing failure usually causes subsequent pacemaker events to release more Ca²⁺ (amplitude/duration), and are likely to result in stronger/more complete activation of the SM. In terms of motility, it is possible (and likely) that "stronger" pacing at half control frequency may impart more useful force to contents and potentially improve motility. As the authors are well aware, this may be the default situation in the stomach (2pacemaker:1contraction). An analysis of a number of common parameters (amplitude, duration, upstroke velocity) performed on each observable event (rather than on all the events in the recording period) would be important to better assess the effects of the drugs used.

2) Interestingly, the authors show a different aspect of the "recovery" effect on the coordination of intracellular release site firing – which is somewhat unexpected, and important. Decreasing extracellular Ca²⁺ began to cause instabilities in the coordination/synchronization in site firing at 1mM while maintaining a frequency close to that observed at 2.5mM. At 0.5mM, firing failure occurred, but in the examples where firing occurred, it was the most synchronous / coordinated observed in the entire experiment. This, again, could indicate that lengthening the duration of "recovery" after a pacemaker event is one of the major factors determining the coordination of both intracellular (site) firing, and by extension, intracellular (network) activity. In contrast to the author's conclusions, this process does not appear to be that dependent on extracellular Ca²⁺ entry and needs to be commented on. In addition, lowering calcium will also affect membrane surface charge, which in itself will affect the electrical field experience by voltage-dependent ion channels.

3) The spatial segmentation of dynamic Ca²⁺ data into PTCLs based on "first" event seems arbitrary, as the degree to which Ca²⁺ spreads from the position of the "first" event appears to be quite variable. The image illustrating individual sites appears to be hand-drawn. If site demarcation was somewhat arbitrary, this should be clarified.

4) The cell density value reported (95 cells per mm2) seems low by an order of magnitude. If the quoted average minimum separation distance is 30µm, one would expect 33 cells across a 1mm in 1D. Using the same separation distance, in 2D, one would expect 33 cells x 33 cells = ~1000 cells per square mm.

5) While a correlation exists between the ICC-SM Ca²⁺ events, smooth muscle Ca²⁺ events and resultant micromotion (Figure 2) in general terms, the traces shown in Figure 2E do not show a 1:1 correlation of the event waveforms. While some variability is expected between micromotions and fluorescence signals due to unknown dynamic/static stress and strain in the gut wall, one would expect the Ca²⁺ event waveforms in ICC-SM and SM to be more correlated given the tight electrical coupling via gap junctions. While all the presented Ca²⁺ events are biphasic in nature, the first event ICC-SM shows a clear separation between the 2 peaks. The time between the 2 peaks appears sufficient to allow for re-stimulation the SM (~1s), given the fluorescence has returned 80-90% back to baseline – yet there is no inflection or change in slope in the SM Ca²⁺ event. The authors should comment on this disparity and qualify under which conditions (frequency/amplitude/? range) the correlation holds true using cross-correlation analysis. Differentiation and distortion vectors would also better locate contraction epicenters in videos and differentiation of traces would provide a more reliable landmark (max dI/dT) to compare time delays.

6) The stomach and small intestine generate slow waves in the outermost ICC layer (myenteric layer: ICC-MY) that co-activates circular and longitudinal muscle layers. Activation of colonic CM from submucosal ICC layer prevents slow waves from directly co-activating longitudinal SM, and this is evident in more independent and varied longitudinal motions (tenia coli tone, greater neural control). The role of colonic ICC-MY is more confusing but appears to synchronize activity in certain conditions, which has been proposed to change the excitability of CM and possibly other layers in the gut wall. As such, without knowing the output from colonic ICC-MY, attempts to correlate CM activity to ICC-SM activity may be highly variable and unreliable. A comment on this would be helpful.

7) The conclusion that Ca²⁺ entry through L-type Ca²⁺ channels is vital to the generation of pacemaker activity is valid with regard to network activity, but the effect on intracellular release sites seems less clear. In Figure 9 A, there are 8-9 well defined "hot spots" demarcated by their white/red color (denoting how long the site was active). The cytoplasm surrounding these hotspots shows a lower occurrence of activity, consistent with an occasional propagating intracellular Ca²⁺ wave. Interestingly, all of the main hotspots remained after nicardipine, albeit firing at a much lower frequency. Wouldn't this suggest that release sites are not intrinsically dependent on L-type Ca²⁺ entry and that the L-Type channels are more involved in facilitating spread of Ca²⁺ through the cytoplasm?

8) Have the authors used an L-type Ca²⁺ channel opener (BayK8644) on the ICC-SM preparation? This would yield important information regarding the role of channel inactivation/"recovery" and the importance of entrainment and limiting ectopic activity on the formation of coherent pacemaker activity.

9) Wouldn't large effect of NNC after nicardipine (Figure 12) suggest that T-type Ca²⁺ channel entry is equally important is L-type entry. The frequency of control activity prior to the addition of NNC (Figure 10) was quite high and is likely to have predisposed cells to firing failure to a much larger degree as slower control frequencies.

10) Raw data ± SE was presented in the both the text and in graphical form, particularly when presenting % data. The text in the Results section or the latter parts of many figures could be reduced considerably and easier to read/ view without the repetition. This would considerably raise the impact of the work.

11) The results of Pinacidil alone (Figure 11) could easily be removed as they didn't reach significance; at the very least, moved to the supplementary section.

12) The effects of pinacidil on Ca channel blockers could perhaps be best illustrated by plotting the red and blue bars in Figure 11 with the normalized results in Figures 9 and 10 to illustrate any effect. “Tendency” statements that should be removed include: “Pinacidil showed a tendency toward increased Ca²⁺ transient firing, but this effect did not reach statistical significance” and “Reduction in the duration of Ca²⁺ transients was associated with a tendency for an increase in frequency, but this parameter did not reach significance”

13) The Xestospongin modulation of the Ca store signalling in ICC-SM is not significant and probably doesn't require illustration or it should be moved to the supplementary data section.

14) The qPCR analyses provide evidence for message for those two channels and the use of three different pharmacological inhibitors (for Cav3) with similar effects (Figure 10, Figure 10—figure supplement 2) support their functional expression. However, can the authors rule out the possibility that NNC 55-0396, TTA-A2 and Z-944 are producing their respective effects on calcium transients by partial inhibition of L-type channels? e.g., does a lower concentration of nicardipine produce similar effects to those agents?

15) Pinacidil was used to activate KATP channels in the muscle layer, when it remained connected to the ICC-SM layer; this presumably(?) led to hyperpolarization of the ICC layer due to electrical coupling between the layers. Is that correct? (If so that assumption was not clearly stated). The differential effects of the calcium channel inhibitors before and after PIN were then used as evidence that voltage-activated calcium channels with more negative thresholds played a role when PIN was used. But shouldn't the effects of isradipine also have been tested in the presence of PIN in order to make conclusions about the contribution of Cav1.3 channels? Supporting electrophysiology data showing the extent of PIN-induced hyperpolarization in ICC-SM cells would aid in interpretation of these data.

16) The authors state that ICC in general do not express KATP channels, but wasn't that analysis made for another type of ICC (ICC-MY?)? If that is so, they should check for Kir6 and SUR expression in these purified ICC-SM cells.

17) Previous studies of ICC-SM in mouse have used 0.01 – 1 μm nifedipine (Yoneda et al., 2002; 2003) and Ni2+ to dissect out contributions of L-and T- type channels to the initial and plateau phases of the slow waves in these cells. That data should be discussed at least to some extent. Do the authors think that the pattern of calcium events (fast and transient vs prolonged) correspond to the initial and sustained phases of the slow waves measured in previous studies, in which it was concluded that T- and L-type calcium channels respectively contributed?

eLife. 2021 Jan 5;10:e64099. doi: 10.7554/eLife.64099.sa2

Author response

Revisions:

1) Paradoxically, firing failure usually causes subsequent pacemaker events to release more Ca²⁺ (amplitude/duration), and are likely to result in stronger/more complete activation of the SM. In terms of motility, it is possible (and likely) that "stronger" pacing at half control frequency may impart more useful force to contents and potentially improve motility. As the authors are well aware, this may be the default situation in the stomach (2pacemaker:1contraction). An analysis of a number of common parameters (amplitude, duration, upstroke velocity) performed on each observable event (rather than on all the events in the recording period) would be important to better assess the effects of the drugs used.

We analyzed the Ca²⁺ firing sites in ICC-SM using particle analysis method. This analysis is based on temporal characteristics to define firing sites. The number of Ca²⁺ firing events is enormous and variable cycle-to-cycle, as firing sites can fire multiple times. The particle analysis method relies on binary transformation to effectively define and segment Ca²⁺ firing sites. Therefore, the intensity amplitude of each firing site is not available, but this binary transformation enables us to effectively quantify the large number of sites within the FOV in terms of temporal information and provides parameters of Ca²⁺ event size, count, duration and frequency.

Following the reviewer's suggestion, in addition to firing sites size and frequency count, we analyzed all data sets under control and drug conditions and added the duration information for Ca²⁺ particle events to aid the readers to better understand the observed drug effects. The event duration data are included in updated figures (Figures 7, 9, 10, 12, 13 and 14) and in the Results section.

2) Interestingly, the authors show a different aspect of the "recovery" effect on the coordination of intracellular release site firing – which is somewhat unexpected, and important. Decreasing extracellular Ca²⁺ began to cause instabilities in the coordination/synchronization in site firing at 1mM while maintaining a frequency close to that observed at 2.5mM. At 0.5mM, firing failure occurred, but in the examples where firing occurred, it was the most synchronous / coordinated observed in the entire experiment. This, again, could indicate that lengthening the duration of "recovery" after a pacemaker event is one of the major factors determining the coordination of both intracellular (site) firing, and by extension, intracellular (network) activity. In contrast to the author's conclusions, this process does not appear to be that dependent on extracellular Ca²⁺ entry and needs to be commented on. In addition, lowering calcium will also affect membrane surface charge, which in itself will affect the electrical field experience by voltage-dependent ion channels.

We agree with the reviewer that reducing [Ca²⁺] _o to 0.5 mM caused a firing failure, which is most likely due to a reduction in Ca²⁺ entry and effectively maintaining store refilling and CICR mechanisms. This observation confirms the role of Ca²⁺ entry in maintaining Ca²⁺ release mechanisms as indicated by the reduction of the number of Ca²⁺ firing sites (Figure 7 K and L)

Following the reviewer’s suggestion, we analyzed the duration of Ca²⁺ firing sites under reduced [Ca²⁺] _o conditions (New Figure 7). There was no significant change in the average individual firing sites duration except when we reduce [Ca²⁺] _o to 1 mM, we noticed a significant increase. This observed increase in Ca²⁺ firing duration at 50% [Ca²⁺] _o may be providing a compensatory mechanism for pacemaker firing failure. Reducing [Ca²⁺] _o further had no significant change in individual firing site duration but the duration of the whole Ca²⁺ transient cycle (CTC) was reduced, and this is in line with previous paper (Ward et al. J Physiology 1992; https://doi.org/10.1113/jphysiol.1992.sp019303) that showed reducing [Ca²⁺] _o to 0.5 mM and 0.25 mM caused a firing failure in colonic slow waves that was accompanied with a reduction in electrical slow wave duration in colonic tissues.

We agree with the reviewer that membrane surface charge could influence some experiments where the divalent ion concentration decreased as [Ca²⁺] _o was reduced. We have addressed this point in a previous paper with a series of cation replacement of Ca²⁺ by Mg²⁺ were tested on colonic electrical slow waves and found no significant differences in the responses (Ward et al., J Physiology 1992).

3) The spatial segmentation of dynamic Ca²⁺ data into PTCLs based on "first" event seems arbitrary, as the degree to which Ca²⁺ spreads from the position of the "first" event appears to be quite variable. The image illustrating individual sites appears to be hand-drawn. If site demarcation was somewhat arbitrary, this should be clarified.

The Ca²⁺ particle analysis used in the study relies on the calculation of the combined area and total number of PTCLs for the entire video. Flags (8-bit, bit-hashed) allowed entire PTCLs to be distinguished based on whether they met certain spatiotemporal (ST) conditions such as size and temporal characteristics. To show the overall regions in cells where Ca²⁺ transients occurred, PTCLs were summed throughout the video to create an image of their occurrence.

Yes. The spatial information in the Ca²⁺ occurrence maps data is an indication of each firing site that gives information on their temporal activation and provides no information on their spatial spread. This was implemented in our analysis to accommodate a large number of firing sites and effectively plot them in a 2D occurrence map. We clarified this point in the Materials and methods section.

4) The cell density value reported (95 cells per mm2) seems low by an order of magnitude. If the quoted average minimum separation distance is 30µm, one would expect 33 cells across a 1mm in 1D. Using the same separation distance, in 2D, one would expect 33 cells x 33 cells = ~1000 cells per square mm.

We thank the reviewer for raising this point. The original minimum separation distances between ICC-SM cells were calculated only with the inclusion of very closely associated ICC-SM cells. The ICC-SM network seems to have multiple processes that can vary in length, as visualized in Figure 1. Therefore, we reanalyzed our images and increased the number of images to provide more accurate cell density measurements. New cell density data was added to the Results section.

5) While a correlation exists between the ICC-SM Ca²⁺ events, smooth muscle Ca²⁺ events and resultant micromotion (Figure 2) in general terms, the traces shown in Figure 2E do not show a 1:1 correlation of the event waveforms. While some variability is expected between micromotions and fluorescence signals due to unknown dynamic/static stress and strain in the gut wall, one would expect the Ca²⁺ event waveforms in ICC-SM and SM to be more correlated given the tight electrical coupling via gap junctions. While all the presented Ca²⁺ events are biphasic in nature, the first event ICC-SM shows a clear separation between the 2 peaks. The time between the 2 peaks appears sufficient to allow for re-stimulation the SM (~1s), given the fluorescence has returned 80-90% back to baseline – yet there is no inflection or change in slope in the SM Ca²⁺ event. The authors should comment on this disparity and qualify under which conditions (frequency/amplitude/? range) the correlation holds true using cross-correlation analysis. Differentiation and distortion vectors would also better locate contraction epicenters in videos and differentiation of traces would provide a more reliable landmark (max dI/dT) to compare time delays.

We agree with the reviewer that the ICC network is coupled to SMCs via gap junctions providing an electric signal from ICC to neighboring SMCs. Because of the syncytial nature of the 2 networks, multiple ICC-SM can influence the activity of any given SMC. Thus, the overall signal in adjacent SMC represents a summation of inputs from many ICC-SM, and this may explain the observation of the reviewer.

In addition, ICC demonstrate complex patterns of Ca²⁺ firing in comparison to SMCs. Ca²⁺ transients in ICC activate ANO1 currents. Conduction of currents and depolarization increases the open probability of L-type Ca²⁺ channels in SMCs, resulting in the Ca²⁺ transients in these cells. The sequence of signals and coupling between Ca²⁺ transients and electrical responses is different in ICC and SMCs. In fact it is opposite: a rise in [Ca²⁺]_o initiates ANO1 currents and depolarization in ICC, and depolarization initiates openings of Ca²⁺ channels and Ca²⁺ entry in SMCs. We don’t anticipate that subtle changes in Ca²⁺ transients in one or a few ICC during a single cycle would have great influence on the Ca²⁺ signals in SMCs, as depolarizing currents from ICC activate Ca²⁺ action potentials. Thus, activation of SMCs may be independent of the exact waveforms of Ca²⁺ transients in ICC as long as threshold is reached. Another factor separating 1:1 waveform behavior in ICC and SMCs is the refractory properties of Ca²⁺ action potentials in SMC and Ca²⁺ in ICC are fundamentally different, causing differences in the temporal and overall “shaping” of responses. To clarify this point, we added comment on the contribution of multiple ICC to the overall SMC signals in the Discussion section.

6) The stomach and small intestine generate slow waves in the outermost ICC layer (myenteric layer: ICC-MY) that co-activates circular and longitudinal muscle layers. Activation of colonic CM from submucosal ICC layer prevents slow waves from directly co-activating longitudinal SM, and this is evident in more independent and varied longitudinal motions (tenia coli tone, greater neural control). The role of colonic ICC-MY is more confusing but appears to synchronize activity in certain conditions, which has been proposed to change the excitability of CM and possibly other layers in the gut wall. As such, without knowing the output from colonic ICC-MY, attempts to correlate CM activity to ICC-SM activity may be highly variable and unreliable. A comment on this would be helpful.

We agree with the reviewer that several types of ICC exist in the colon, and all, through coupling of Ca²⁺ transients and activation of ANO1 channels, can contribute to colonic excitability. Both ICC-SM and ICC-MY provide pacemaker activities in the colon. To understand the complex behaviors of these cells, a reductionist approach is first needed. In this study we focused on the intrinsic pacemaker role of ICC-SM in driving adjacent smooth muscle. Most of the experiments in the study, used an isolated ICC-SM preparation to define Ca²⁺ signals in ICC-SM with minimal motion artifacts. To examine if ICC-SM drive activity in CM, we used a preparation that included attached SMCs. It is true these preparations included additional types of ICC that might also influence the ultimate behaviors of SMCs. Therefore, we cannot exclude the fact that the signals in SMCs under the conditions of these specific experiments are influenced by additional types of ICC. We know from this study and previously published papers that ICC-SM in the mouse colon generates slow waves at ~ 15 /min whereas ICC-MY ~2/min. Contractions observed in our experiments followed the frequency of behaviors in ICC-SM. Possible influences from additional cells within the muscle tissues were not evaluated in this study. To fully understand the highly integrated functions in colonic motility that result from the behaviors of many types of cells: ICC, SMCs and interstitial cells labeled with antibodies to PDGFRα (i.e. the SIP syncytium), enteric neural inputs and tuning of neural activity by glial cells, will require considerably more complex imaging approaches and refined techniques to limit movements without interfering with the electrical behaviors and Ca²⁺ dynamics of the cells involved.

At this point, we do not know if the activity of ICC-SM propagates through the CM to influence the behaviors of ICC-MY and longitudinal muscle or vice versa. Previous studies in larger animals have shown that pacemaker propagation declines as a function of distance from the submucosal region towards the myenteric region. Smith et al., 1987 AJP.

7) The conclusion that Ca²⁺ entry through L-type Ca²⁺ channels is vital to the generation of pacemaker activity is valid with regard to network activity, but the effect on intracellular release sites seems less clear. In Figure 9 A, there are 8-9 well defined "hot spots" demarcated by their white/red color (denoting how long the site was active). The cytoplasm surrounding these hotspots shows a lower occurrence of activity, consistent with an occasional propagating intracellular Ca²⁺ wave. Interestingly, all of the main hotspots remained after nicardipine, albeit firing at a much lower frequency. Wouldn't this suggest that release sites are not intrinsically dependent on L-type Ca²⁺ entry and that the L-Type channels are more involved in facilitating spread of Ca²⁺ through the cytoplasm?

After the addition of nicardipine a few remnant Ca²⁺ release sites continued to fire throughout experiments (hot-spots). But after nicardipine these sites fired randomly, and their frequency was very low. Thus, we interpret these observations to mean that Ca²⁺ release is an intrinsic property of ICC, and it occurs from specific sites within cells. Ca²⁺ influx enhances the excitability of Ca²⁺ sites and is crucial for organizing Ca²⁺ release into clusters that cause sustained activation of ANO1 channels and electrical depolarizations (slow waves). Ca²⁺ entry organizes Ca²⁺ release via CICR. Ca²⁺ entry through L-type Ca²⁺ channels may also contribute to refilling of stores.

8) Have the authors used an L-type Ca²⁺ channel opener (BayK8644) on the ICC-SM preparation? This would yield important information regarding the role of channel inactivation/"recovery" and the importance of entrainment and limiting ectopic activity on the formation of coherent pacemaker activity.

We have used the L-type channel antagonist (Nicardipine) in this study to evaluate contributions of these channels, however we have not used an L-type channel agonist, such as BayK8644. It is an interesting suggestion, and we will utilize this compound in future studies.

9) Wouldn't large effect of NNC after nicardipine (Figure 12) suggest that T-type Ca²⁺ channel entry is equally important is L-type entry. The frequency of control activity prior to the addition of NNC (Figure 10) was quite high and is likely to have predisposed cells to firing failure to a much larger degree as slower control frequencies.

We agree with the reviewer that both L-type and T-type Ca²⁺ channels are present and may contribute to pacemaker activity in ICC-SM. However, the resting membrane potential of colonic muscles is usually not at highly negative potentials (e.g. ~45 mV; Koh et al. Neurogastroenterol Motil. 2012: doi: 10.1111/j.1365-2982.2012.01956.x). Thus, the relative importance of T-type Ca²⁺ channels may be reduced due to tonic inactivation of these channels. In cases of depolarized cells, L-type Ca²⁺ channels may dominate, as appears to happen under resting conditions in our experiments. T-type Ca²⁺ channels, however, can become more important if membrane potential is hyperpolarized, as indicated by our experiments when pinacidil was used to hyperpolarize cells. This shift in importance between the two conductances could be important in pathophysiological conditions associated with changes in the membrane potential. Having two conductance expressed with different voltage-dependencies provides a safety factor for maintenance of pacemaker activity, an important and novel observation of the present study.

10) Raw data ± SE was presented in the both the text and in graphical form, particularly when presenting % data. The text in the Results section or the latter parts of many figures could be reduced considerably and easier to read/ view without the repetition. This would considerably raise the impact of the work.

We thank the reviewer for this suggestion, we feel that including the Raw data ± SE in the Results section would provide the reader of details on the degree of drug effects and help in tabulate the results presented.

11) The results of Pinacidil alone (Figure 11) could easily be removed as they didn't reach significance; at the very least, moved to the supplementary section.

Following the reviewer's suggestion, we removed the effects of pinacidil alone to a new supplementary figure (Figure 11—figure supplement 1).

12) The effects of pinacidil on Ca channel blockers could perhaps be best illustrated by plotting the red and blue bars in Figure 11 with the normalized results in Figures 9 and 10 to illustrate any effect. “Tendency” statements that should be removed include: “Pinacidil showed a tendency toward increased Ca²⁺ transient firing, but this effect did not reach statistical significance” and “Reduction in the duration of Ca²⁺ transients was associated with a tendency for an increase in frequency, but this parameter did not reach significance”

Following the reviewer's suggestion, we removed the effects of pinacidil alone to a new supplementary figure. We also removed all tendency statements from the Results section.

13) The Xestospongin modulation of the Ca store signalling in ICC-SM is not significant and probably doesn't require illustration or it should be moved to the supplementary data section.

Following the reviewer's suggestion, we removed the effects of Xestospongin into a new supplementary figure (Figure 13—figure supplement 1).

14) The qPCR analyses provide evidence for message for those two channels and the use of three different pharmacological inhibitors (for Cav3) with similar effects (Figure 10, Figure 10—figure supplement 1) support their functional expression. However, can the authors rule out the possibility that NNC 55-0396, TTA-A2 and Z-944 are producing their respective effects on calcium transients by partial inhibition of L-type channels? e.g., does a lower concentration of nicardipine produce similar effects to those agents?

A previous report has examined the selectivity of these drugs. Ming li et al. 2005 (DOI: 10.1111/j.1527-3466.2005.tb00164.x) demonstrated that 100 microM NNC 55-0396 has no detectable effect on high voltage-activated currents and does not produce metabolite that causes inhibition of L-type Ca²⁺ channel.

15) Pinacidil was used to activate KATP channels in the muscle layer, when it remained connected to the ICC-SM layer; this presumably(?) led to hyperpolarization of the ICC layer due to electrical coupling between the layers. Is that correct? (If so that assumption was not clearly stated). The differential effects of the calcium channel inhibitors before and after PIN were then used as evidence that voltage-activated calcium channels with more negative thresholds played a role when PIN was used. But shouldn't the effects of isradipine also have been tested in the presence of PIN in order to make conclusions about the contribution of Cav1.3 channels? Supporting electrophysiology data showing the extent of PIN-induced hyperpolarization in ICC-SM cells would aid in interpretation of these data.

In the current study, pinacidil application was used to produce a membrane hyperpolarization in SMCs via activation of KATP channels and ICC as they are electrically coupled. We added a statement in the Results section to clarify the rationale of pinacidil application.

We agree with the reviewer point that these experiments do not directly incorporate potential involvement of Cav 1.3 channels. Data in this study show that ICC-SM Ca²⁺ transients depend upon Ca²⁺ influx via L-type Ca²⁺ channels, and since isradipine had nearly the same or a lesser effect on Ca²⁺ transients than nicardipine, Ca_V 1.2 appear dominant as the Ca²⁺ entry mechanism. This set of experiments were targeted to determine the contributions of T-type Ca²⁺ channels. The results demonstrate that at negative potentials, where the open probability of L-type Ca²⁺ channels is low, T-type Ca²⁺ channels are available to facilitate Ca²⁺ influx and sustain pacemaker activity. We agree that patch clamp experiments would further support the conclusions, and we will incorporate them in future studies.

16) The authors state that ICC in general do not express KATP channels, but wasn't that analysis made for another type of ICC (ICC-MY?)? If that is so, they should check for Kir6 and SUR expression in these purified ICC-SM cells.

ICC express message for Kir6 and SUR, however KATP currents cannot be resolved in colonic ICC (Huang et al. 2018). At present it is unknown whether Kir6 and SUR are not translated or not trafficked to the plasma membranes to create functional KATP channels in ICC.

17) Previous studies of ICC-SM in mouse have used 0.01 – 1 μm nifedipine (Yoneda et al., 2002; 2003) and Ni2+ to dissect out contributions of L-and T- type channels to the initial and plateau phases of the slow waves in these cells. That data should be discussed at least to some extent. Do the authors think that the pattern of calcium events (fast and transient vs prolonged) correspond to the initial and sustained phases of the slow waves measured in previous studies, in which it was concluded that T- and L-type calcium channels respectively contributed?

Both of Yoneda et al. papers (Yoneda et al., 2002; 2003) concluded that; L-type and T-type Ca²⁺ channels contribute to slow waves in the colon. L-type Ca²⁺ channels completely abolished slow waves where T-type channels are involved in the initial component of the slow wave. Our study highlighting the Ca²⁺ signaling in ICC-SM is consistent with conclusions of the Yoneda et al. papers. Although the comparison between the electrical activity and Ca²⁺ dynamics from different studies is difficult to clearly interpret. Remember that the electrical activity was recorded (most likely) from SMCs and we are imaging Ca²⁺ dynamics in most experiments exclusively from ICC. Figure 15 summarizes the sequence of events in ICC, and these events drive the depolarization-dependent activation of Ca²⁺ action potentials/ Ca²⁺ entry in SMCs. We have taken the reviewer’s suggestion and added more information about the conclusions of Yoneda et al. in the Discussion.

Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Supplementary file 1. Table summary of gene primers sequences of Kit, Ano1, Myh11, Uchl1, Cacna1c, Cacna1d, Cacna1g, Cacna1h, and Orai1-3.

elife-64099-supp1.docx^{(14.3KB, docx)}

Transparent reporting form

elife-64099-transrepform.docx^{(246.3KB, docx)}

Data Availability Statement

All data generated or analysed during this study are included in the manuscript and supporting files.

[bib1] Amberg GC, Navedo MF, Santana LF. On the loose: uncaging Ca2+-induced Ca2+ release in smooth muscle. Journal of General Physiology. 2006;127:221–223. doi: 10.1085/jgp.200609510. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib2] Anekonda TS, Quinn JF, Harris C, Frahler K, Wadsworth TL, Woltjer RL. L-type voltage-gated calcium channel blockade with isradipine as a therapeutic strategy for alzheimer's disease. Neurobiology of Disease. 2011;41:62–70. doi: 10.1016/j.nbd.2010.08.020. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib3] Baker SA, Hennig GW, Ward SM, Sanders KM. Temporal sequence of activation of cells involved in Purinergic neurotransmission in the Colon. The Journal of Physiology. 2015;593:1945–1963. doi: 10.1113/jphysiol.2014.287599. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib4] Baker SA, Drumm BT, Saur D, Hennig GW, Ward SM, Sanders KM. Spontaneous ca(2+) transients in interstitial cells of cajal located within the deep muscular plexus of the murine small intestine. The Journal of Physiology. 2016;594:3317–3338. doi: 10.1113/JP271699. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib5] Bayguinov O, Ward SM, Kenyon JL, Sanders KM. Voltage-gated Ca2+ currents are necessary for slow-wave propagation in the canine gastric antrum. American Journal of Physiology. Cell Physiology. 2007;293:C1645–C1659. doi: 10.1152/ajpcell.00165.2007. [DOI] [PubMed] [Google Scholar]

[bib6] Beckett EA, Bayguinov YR, Sanders KM, Ward SM, Hirst GD. Properties of unitary potentials generated by intramuscular interstitial cells of cajal in the murine and guinea-pig gastric fundus. The Journal of Physiology. 2004;559:259–269. doi: 10.1113/jphysiol.2004.063016. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib7] Bell DC, Butcher AJ, Berrow NS, Page KM, Brust PF, Nesterova A, Stauderman KA, Seabrook GR, Nürnberg B, Dolphin AC. Biophysical properties, pharmacology, and modulation of human, neuronal L-type (alpha(1D), Ca(V)1.3) voltage-dependent calcium currents. Journal of Neurophysiology. 2001;85:816–827. doi: 10.1152/jn.2001.85.2.816. [DOI] [PubMed] [Google Scholar]

[bib8] Berezin I, Huizinga JD, Daniel EE. Interstitial cells of cajal in the canine Colon: a special communication network at the inner border of the circular muscle. The Journal of Comparative Neurology. 1988;273:42–51. doi: 10.1002/cne.902730105. [DOI] [PubMed] [Google Scholar]

[bib9] Berjukow S, Marksteiner R, Gapp F, Sinnegger MJ, Hering S. Molecular mechanism of calcium channel block by isradipine role of a drug-induced inactivated channel conformation. The Journal of Biological Chemistry. 2000;275:22114–22120. doi: 10.1074/jbc.M908836199. [DOI] [PubMed] [Google Scholar]

[bib10] Berridge MJ. Inositol trisphosphate and calcium signalling mechanisms. Biochimica Et Biophysica Acta (BBA) - Molecular Cell Research. 2009;1793:933–940. doi: 10.1016/j.bbamcr.2008.10.005. [DOI] [PubMed] [Google Scholar]

[bib11] Burns AJ, Lomax AE, Torihashi S, Sanders KM, Ward SM. Interstitial cells of cajal mediate inhibitory neurotransmission in the stomach. PNAS. 1996;93:12008–12013. doi: 10.1073/pnas.93.21.12008. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib12] Burns AJ, Herbert TM, Ward SM, Sanders KM. Interstitial cells of cajal in the guinea-pig gastrointestinal tract as revealed by c-Kit immunohistochemistry. Cell and Tissue Research. 1997;290:11–20. doi: 10.1007/s004410050902. [DOI] [PubMed] [Google Scholar]

[bib13] Butorac C, Krizova A, Derler I. Review: structure and activation mechanisms of CRAC channels. Advances in Experimental Medicine and Biology. 2020;1131:547–604. doi: 10.1007/978-3-030-12457-1_23. [DOI] [PubMed] [Google Scholar]

[bib14] Chauvet S, Jarvis L, Chevallet M, Shrestha N, Groschner K, Bouron A. Pharmacological characterization of the native Store-Operated calcium channels of cortical neurons from embryonic mouse brain. Frontiers in Pharmacology. 2016;7:486. doi: 10.3389/fphar.2016.00486. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib15] Chen J, Sanderson MJ. Store-operated calcium entry is required for sustained contraction and Ca2+ oscillations of airway smooth muscle. The Journal of Physiology. 2017;595:3203–3218. doi: 10.1113/JP272694. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib16] Corsetti M, Costa M, Bassotti G, Bharucha AE, Borrelli O, Dinning P, Di Lorenzo C, Huizinga JD, Jimenez M, Rao S, Spiller R, Spencer NJ, Lentle R, Pannemans J, Thys A, Benninga M, Tack J. First translational consensus on terminology and definitions of colonic motility in animals and humans studied by manometric and other techniques. Nature Reviews Gastroenterology & Hepatology. 2019;16:559–579. doi: 10.1038/s41575-019-0167-1. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib17] Cousins HM, Edwards FR, Hickey H, Hill CE, Hirst GD. Electrical coupling between the myenteric interstitial cells of cajal and adjacent muscle layers in the guinea-pig gastric antrum. The Journal of Physiology. 2003;550:829–844. doi: 10.1113/jphysiol.2003.042176. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib18] D'Antona G, Hennig GW, Costa M, Humphreys CM, Brookes SJ. Analysis of motor patterns in the isolated guinea-pig large intestine by spatio-temporal maps. Neurogastroenterology and Motility. 2001;13:483–492. doi: 10.1046/j.1365-2982.2001.00282.x. [DOI] [PubMed] [Google Scholar]

[bib19] Derler I, Schindl R, Fritsch R, Heftberger P, Riedl MC, Begg M, House D, Romanin C. The action of selective CRAC channel blockers is affected by the orai pore geometry. Cell Calcium. 2013;53:139–151. doi: 10.1016/j.ceca.2012.11.005. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib20] Drumm BT, Hennig GW, Battersby MJ, Cunningham EK, Sung TS, Ward SM, Sanders KM, Baker SA. Clustering of Ca2+ transients in interstitial cells of cajal defines slow wave duration. Journal of General Physiology. 2017;149:703–725. doi: 10.1085/jgp.201711771. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib21] Drumm BT, Hwang SJ, Baker SA, Ward SM, Sanders KM. Ca2+ signalling behaviours of intramuscular interstitial cells of cajal in the murine Colon. The Journal of Physiology. 2019a;597:3587–3617. doi: 10.1113/JP278036. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib22] Drumm BT, Hennig GW, Baker SA, Sanders KM. Applications of Spatio-temporal mapping and particle analysis techniques to quantify intracellular Ca2+ signaling in situ. Journal of Visualized Experiments. 2019b;7:58989. doi: 10.3791/58989. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib23] Drumm BT, Rembetski BE, Messersmith K, Manierka MS, Baker SA, Sanders KM. Pacemaker function and neural responsiveness of subserosal interstitial cells of cajal in the mouse Colon. The Journal of Physiology. 2020a;598:651–681. doi: 10.1113/JP279102. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib24] Drumm BT, Rembetski BE, Huynh K, Nizar A, Baker SA, Sanders KM. Excitatory cholinergic responses in mouse Colon intramuscular interstitial cells of cajal are due to enhanced ca. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. 2020b;34:10073–10095. doi: 10.1096/fj.202000672R. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib25] Dupont G, Goldbeter A. Properties of intracellular Ca2+ waves generated by a model based on ca(2+)-induced Ca2+ release. Biophysical Journal. 1994;67:2191–2204. doi: 10.1016/S0006-3495(94)80705-2. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib26] Durdle NG, Kingma YJ, Bowes KL, Chambers MM. Origin of slow waves in the canine Colon. Gastroenterology. 1983;84:375–382. doi: 10.1016/S0016-5085(83)80136-X. [DOI] [PubMed] [Google Scholar]

[bib27] Faussone Pellegrini MS, Cortesini C, Romagnoli P. Ultrastructure of the Tunica muscularis of the cardial portion of the human esophagus and stomach, with special reference to the so-called cajal's interstitial cells] Archivio Italiano Di Anatomia E Di Embriologia. Italian Journal of Anatomy and Embryology. 1977;82:157–177. [PubMed] [Google Scholar]

[bib28] Faussone-Pellegrini MS. Histogenesis, structure and relationships of interstitial cells of cajal (ICC): from morphology to functional interpretation. European Journal of Morphology. 1992;30:137–148. [PubMed] [Google Scholar]

[bib29] Fill M, Copello JA. Ryanodine receptor calcium release channels. Physiological Reviews. 2002;82:893–922. doi: 10.1152/physrev.00013.2002. [DOI] [PubMed] [Google Scholar]

[bib30] Gallego D, Gil V, Aleu J, Aulí M, Clavé P, Jiménez M. Purinergic and nitrergic junction potential in the human Colon. American Journal of Physiology-Gastrointestinal and Liver Physiology. 2008;295:G522–G533. doi: 10.1152/ajpgi.00510.2007. [DOI] [PubMed] [Google Scholar]

[bib31] Gibson A, McFadzean I, Wallace P, Wayman CP. Capacitative Ca2+ entry and the regulation of smooth muscle tone. Trends in Pharmacological Sciences. 1998;19:266–269. doi: 10.1016/s0165-6147(98)01222-x. [DOI] [PubMed] [Google Scholar]

[bib32] Gomez-Pinilla PJ, Gibbons SJ, Bardsley MR, Lorincz A, Pozo MJ, Pasricha PJ, Van de Rijn M, West RB, Sarr MG, Kendrick ML, Cima RR, Dozois EJ, Larson DW, Ordog T, Farrugia G. Ano1 is a selective marker of interstitial cells of cajal in the human and mouse gastrointestinal tract. American Journal of Physiology-Gastrointestinal and Liver Physiology. 2009;296:G1370–G1381. doi: 10.1152/ajpgi.00074.2009. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib33] Hara Y, Kubota M, Szurszewski JH. Electrophysiology of smooth muscle of the small intestine of some mammals. The Journal of Physiology. 1986;372:501–520. doi: 10.1113/jphysiol.1986.sp016022. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib34] Hennig GW, Hirst GD, Park KJ, Smith CB, Sanders KM, Ward SM, Smith TK. Propagation of pacemaker activity in the guinea-pig antrum. The Journal of Physiology. 2004;556:585–599. doi: 10.1113/jphysiol.2003.059055. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib35] Hirano Y, Fozzard HA, January CT. Characteristics of L- and T-type Ca2+ currents in canine cardiac purkinje cells. American Journal of Physiology-Heart and Circulatory Physiology. 1989;256:H1478–H1492. doi: 10.1152/ajpheart.1989.256.5.H1478. [DOI] [PubMed] [Google Scholar]

[bib36] Hotta A, Okada N, Suzuki H. Mibefradil-sensitive component involved in the plateau potential in submucosal interstitial cells of the murine proximal Colon. Biochemical and Biophysical Research Communications. 2007;353:170–176. doi: 10.1016/j.bbrc.2006.12.003. [DOI] [PubMed] [Google Scholar]

[bib37] Huang X, Lee SH, Lu H, Sanders KM, Koh SD. Molecular and functional characterization of inwardly rectifying K + currents in murine proximal Colon. The Journal of Physiology. 2018;596:379–391. doi: 10.1113/JP275234. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib38] Huizinga JD, Shin A, Chow E. Electrical coupling and pacemaker activity in colonic smooth muscle. American Journal of Physiology-Cell Physiology. 1988;255:C653–C660. doi: 10.1152/ajpcell.1988.255.5.C653. [DOI] [PubMed] [Google Scholar]

[bib39] Huizinga JD, Thuneberg L, Klüppel M, Malysz J, Mikkelsen HB, Bernstein A. W/kit gene required for interstitial cells of cajal and for intestinal pacemaker activity. Nature. 1995;373:347–349. doi: 10.1038/373347a0. [DOI] [PubMed] [Google Scholar]

[bib40] Hwang SJ, Blair PJ, Britton FC, O'Driscoll KE, Hennig G, Bayguinov YR, Rock JR, Harfe BD, Sanders KM, Ward SM. Expression of anoctamin 1/TMEM16A by interstitial cells of cajal is fundamental for slow wave activity in gastrointestinal muscles. The Journal of Physiology. 2009;587:4887–4904. doi: 10.1113/jphysiol.2009.176198. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib41] Hwang SJ, Blair PJ, Durnin L, Mutafova-Yambolieva V, Sanders KM, Ward SM. P2Y1 purinoreceptors are fundamental to inhibitory motor control of murine colonic excitability and transit. The Journal of Physiology. 2012;590:1957–1972. doi: 10.1113/jphysiol.2011.224634. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib42] Ishikawa K, Komuro T. Characterization of the interstitial cells associated with the submuscular plexus of the guinea-pig Colon. Anatomy and Embryology. 1996;194:49–55. doi: 10.1007/BF00196314. [DOI] [PubMed] [Google Scholar]

[bib43] Kaßmann M, Szijártó IA, García-Prieto CF, Fan G, Schleifenbaum J, Anistan YM, Tabeling C, Shi Y, le Noble F, Witzenrath M, Huang Y, Markó L, Nelson MT, Gollasch M. Role of ryanodine type 2 receptors in elementary ca. Journal of the American Heart Association. 2019;8:e010090. doi: 10.1161/JAHA.118.010090. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib44] Keef KD, Ward SM, Stevens RJ, Frey BW, Sanders KM. Electrical and mechanical effects of acetylcholine and substance P in subregions of canine Colon. American Journal of Physiology-Gastrointestinal and Liver Physiology. 1992;262:G298–G307. doi: 10.1152/ajpgi.1992.262.2.G298. [DOI] [PubMed] [Google Scholar]

[bib45] Keef KD, Anderson U, O'Driscoll K, Ward SM, Sanders KM. Electrical activity induced by nitric oxide in canine colonic circular muscle. American Journal of Physiology-Gastrointestinal and Liver Physiology. 2002;282:G123–G129. doi: 10.1152/ajpgi.00217.2001. [DOI] [PubMed] [Google Scholar]

[bib46] Kito Y, Ward SM, Sanders KM. Pacemaker potentials generated by interstitial cells of cajal in the murine intestine. American Journal of Physiology-Cell Physiology. 2005;288:C710–C720. doi: 10.1152/ajpcell.00361.2004. [DOI] [PubMed] [Google Scholar]

[bib47] Kito Y, Suzuki H. Modulation of slow waves by hyperpolarization with potassium channel openers in antral smooth muscle of the guinea-pig stomach. The Journal of Physiology. 2003;548:175–189. doi: 10.1113/jphysiol.2002.035550. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib48] Koh SD, Bradley KK, Rae MG, Keef KD, Horowitz B, Sanders KM. Basal activation of ATP-sensitive potassium channels in murine colonic smooth muscle cell. Biophysical Journal. 1998;75:1793–1800. doi: 10.1016/S0006-3495(98)77621-0. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib49] Komuro T, Tokui K, Zhou DS. Identification of the interstitial cells of cajal. Histology and Histopathology. 1996;11:769–786. [PubMed] [Google Scholar]

[bib50] Komuro T. Comparative morphology of interstitial cells of cajal: ultrastructural characterization. Microscopy Research and Technique. 1999;47:267–285. doi: 10.1002/(SICI)1097-0029(19991115)47:4<267::AID-JEMT5>3.0.CO;2-O. [DOI] [PubMed] [Google Scholar]

[bib51] Komuro T. Structure and organization of interstitial cells of cajal in the gastrointestinal tract. The Journal of Physiology. 2006;576:653–658. doi: 10.1113/jphysiol.2006.116624. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib52] Koschak A, Reimer D, Huber I, Grabner M, Glossmann H, Engel J, Striessnig J. alpha 1d (Cav1.3) Subunits Can Form L-type Ca 2+Channels Activating at Negative Voltages. Journal of Biological Chemistry. 2001;276:22100–22106. doi: 10.1074/jbc.M101469200. [DOI] [PubMed] [Google Scholar]

[bib53] Langton P, Ward SM, Carl A, Norell MA, Sanders KM. Spontaneous electrical activity of interstitial cells of cajal isolated from canine proximal Colon. PNAS. 1989;86:7280–7284. doi: 10.1073/pnas.86.18.7280. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib54] Lee MY, Ha SE, Park C, Park PJ, Fuchs R, Wei L, Jorgensen BG, Redelman D, Ward SM, Sanders KM, Ro S. Transcriptome of interstitial cells of cajal reveals unique and selective gene signatures. PLOS ONE. 2017;12:e0176031. doi: 10.1371/journal.pone.0176031. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib55] Leigh WA, Del Valle G, Kamran SA, Drumm BT, Tavakkoli A, Sanders KM, Baker SA. A high throughput machine-learning driven analysis of ca. Cell Calcium. 2020;91:102260. doi: 10.1016/j.ceca.2020.102260. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib56] Lies B, Gil V, Groneberg D, Seidler B, Saur D, Wischmeyer E, Jiménez M, Friebe A. Interstitial cells of cajal mediate nitrergic inhibitory neurotransmission in the murine gastrointestinal tract. American Journal of Physiology-Gastrointestinal and Liver Physiology. 2014;307:G98–G106. doi: 10.1152/ajpgi.00082.2014. [DOI] [PubMed] [Google Scholar]

[bib57] Lyfenko AD, Dirksen RT. Differential dependence of store-operated and excitation-coupled Ca2+ entry in skeletal muscle on STIM1 and Orai1. The Journal of Physiology. 2008;586:4815–4824. doi: 10.1113/jphysiol.2008.160481. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib58] Navedo MF, Amberg GC. Local regulation of L-type Ca²⁺ channel sparklets in arterial smooth muscle. Microcirculation. 2013a;20:290–298. doi: 10.1111/micc.12021. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib59] Navedo MF, Santana LF. CaV1.2 sparklets in heart and vascular smooth muscle. Journal of Molecular and Cellular Cardiology. 2013b;58:67–76. doi: 10.1016/j.yjmcc.2012.11.018. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib60] Nowycky MC, Fox AP, Tsien RW. Three types of neuronal calcium channel with different calcium agonist sensitivity. Nature. 1985;316:440–443. doi: 10.1038/316440a0. [DOI] [PubMed] [Google Scholar]

[bib61] Park KJ, Hennig GW, Lee HT, Spencer NJ, Ward SM, Smith TK, Sanders KM. Spatial and temporal mapping of pacemaker activity in interstitial cells of cajal in mouse ileum in situ. American Journal of Physiology-Cell Physiology. 2006;290:C1411–C1427. doi: 10.1152/ajpcell.00447.2005. [DOI] [PubMed] [Google Scholar]

[bib62] Plujà L, Albertí E, Fernández E, Mikkelsen HB, Thuneberg L, Jiménez M. Evidence supporting presence of two pacemakers in rat Colon. American Journal of Physiology-Gastrointestinal and Liver Physiology. 2001;281:G255–G266. doi: 10.1152/ajpgi.2001.281.1.G255. [DOI] [PubMed] [Google Scholar]

[bib63] Prakriya M, Lewis RS. Store-Operated calcium channels. Physiological Reviews. 2015;95:1383–1436. doi: 10.1152/physrev.00020.2014. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib64] Putney JW. The physiological function of store-operated calcium entry. Neurochemical Research. 2011;36:1157–1165. doi: 10.1007/s11064-010-0383-0. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib65] Putney JW. Forms and functions of store-operated calcium entry mediators, STIM and orai. Advances in Biological Regulation. 2018;68:88–96. doi: 10.1016/j.jbior.2017.11.006. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib66] Ro S, Park C, Jin J, Zheng H, Blair PJ, Redelman D, Ward SM, Yan W, Sanders KM. A model to study the phenotypic changes of interstitial cells of cajal in gastrointestinal diseases. Gastroenterology. 2010;138:1068–1078. doi: 10.1053/j.gastro.2009.11.007. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib67] Rosli RM, Heitmann PT, Kumar R, Hibberd TJ, Costa M, Wiklendt L, Wattchow DA, Arkwright J, de Fontgalland D, Brookes SJH, Spencer NJ, Dinning PG. Distinct patterns of myogenic motor activity identified in isolated human distal Colon with high-resolution manometry. Neurogastroenterology & Motility. 2020;32:e13871. doi: 10.1111/nmo.13871. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib68] Rüegg UT, Hof RP. Pharmacology of the calcium antagonist isradipine. Drugs. 1990;40:3–9. doi: 10.2165/00003495-199000402-00004. [DOI] [PubMed] [Google Scholar]

[bib69] Rumessen JJ, Vanderwinden JM, Hansen A, Horn T. Ultrastructure of interstitial cells in subserosa of human Colon. Cells Tissues Organs. 2013;197:322–332. doi: 10.1159/000346314. [DOI] [PubMed] [Google Scholar]

[bib70] Rumessen JJ, Thuneberg L. Plexus muscularis profundus and associated interstitial cells. I. light microscopical studies of mouse small intestine. The Anatomical Record. 1982;203:115–127. doi: 10.1002/ar.1092030111. [DOI] [PubMed] [Google Scholar]

[bib71] Sanders KM, Stevens R, Burke E, Ward SW. Slow waves actively propagate at Submucosal surface of circular layer in canine Colon. American Journal of Physiology-Gastrointestinal and Liver Physiology. 1990;259:G258–G263. doi: 10.1152/ajpgi.1990.259.2.G258. [DOI] [PubMed] [Google Scholar]

[bib72] Sanders KM, Hwang SJ, Ward SM. Neuroeffector apparatus in gastrointestinal smooth muscle organs. The Journal of Physiology. 2010;588:4621–4639. doi: 10.1113/jphysiol.2010.196030. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib73] Sanders KM, Koh SD, Ro S, Ward SM. Regulation of gastrointestinal motility--insights from smooth muscle biology. Nature Reviews Gastroenterology & Hepatology. 2012;9:633–645. doi: 10.1038/nrgastro.2012.168. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib74] Sanders KM, Ward SM, Koh SD. Interstitial cells: regulators of smooth muscle function. Physiological Reviews. 2014a;94:859–907. doi: 10.1152/physrev.00037.2013. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib75] Sanders KM, Salter AK, Hennig GW, Koh SD, Perrino BA, Ward SM, Baker SA. Responses to enteric motor neurons in the gastric fundus of mice with reduced intramuscular interstitial cells of cajal. Journal of Neurogastroenterology and Motility. 2014b;20:171–184. doi: 10.5056/jnm.2014.20.2.171. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib76] Santana LF, Navedo MF, Amberg GC, Nieves-Cintrón M, Votaw VS, Ufret-Vincenty CA. Calcium sparklets in arterial smooth muscle. Clinical and Experimental Pharmacology and Physiology. 2008;35:1121–1126. doi: 10.1111/j.1440-1681.2007.04867.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib77] Scholze A, Plant TD, Dolphin AC, Nürnberg B. Functional expression and characterization of a voltage-gated CaV1.3 (alpha1D) calcium channel subunit from an insulin-secreting cell line. Molecular Endocrinology. 2001;15:1211–1221. doi: 10.1210/mend.15.7.0666. [DOI] [PubMed] [Google Scholar]

[bib78] Serio R, Barajas-Lopez C, Daniel EE, Berezin I, Huizinga JD. Slow-wave activity in Colon: role of network of submucosal interstitial cells of cajal. American Journal of Physiology-Gastrointestinal and Liver Physiology. 1991;260:G636–G645. doi: 10.1152/ajpgi.1991.260.4.G636. [DOI] [PubMed] [Google Scholar]

[bib79] Smith TK, Reed JB, Sanders KM. Origin and propagation of electrical slow waves in circular muscle of canine proximal Colon. American Journal of Physiology-Cell Physiology. 1987a;252:C215–C224. doi: 10.1152/ajpcell.1987.252.2.C215. [DOI] [PubMed] [Google Scholar]

[bib80] Smith TK, Reed JB, Sanders KM. Interaction of two electrical pacemakers in muscularis of canine proximal Colon. American Journal of Physiology-Cell Physiology. 1987b;252:C290–C299. doi: 10.1152/ajpcell.1987.252.3.C290. [DOI] [PubMed] [Google Scholar]

[bib81] Sonkusare SK, Bonev AD, Ledoux J, Liedtke W, Kotlikoff MI, Heppner TJ, Hill-Eubanks DC, Nelson MT. Elementary Ca2+ signals through endothelial TRPV4 channels regulate vascular function. Science. 2012;336:597–601. doi: 10.1126/science.1216283. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib82] Takeda Y, Nystoriak MA, Nieves-Cintrón M, Santana LF, Navedo MF. Relationship between Ca2+ sparklets and sarcoplasmic reticulum Ca2+ load and release in rat cerebral arterial smooth muscle. American Journal of Physiology. Heart and Circulatory Physiology. 2011;301:H2285–H2294. doi: 10.1152/ajpheart.00488.2011. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib83] Thomas AP, Bird GS, Hajnóczky G, Robb-Gaspers LD, Putney JW. Spatial and temporal aspects of cellular calcium signaling. The FASEB Journal. 1996;10:1505–1517. doi: 10.1096/fasebj.10.13.8940296. [DOI] [PubMed] [Google Scholar]

[bib84] Thuneberg L. Interstitial cells of cajal: intestinal pacemaker cells? Advances in Anatomy, Embryology, and Cell Biology. 1982;71:1–130. [PubMed] [Google Scholar]

[bib85] Trebak M, Zhang W, Ruhle B, Henkel MM, González-Cobos JC, Motiani RK, Stolwijk JA, Newton RL, Zhang X. What role for store-operated Ca²⁺ entry in muscle? Microcirculation. 2013;20:330–336. doi: 10.1111/micc.12042. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib86] Tseng GN, Boyden PA. Multiple types of Ca2+ currents in single canine purkinje cells. Circulation Research. 1989;65:1735–1750. doi: 10.1161/01.RES.65.6.1735. [DOI] [PubMed] [Google Scholar]

[bib87] Vanderwinden JM, Rumessen JJ, Liu H, Descamps D, De Laet MH, Vanderhaeghen JJ. Interstitial cells of cajal in human Colon and in Hirschsprung's disease. Gastroenterology. 1996;111:901–910. doi: 10.1016/S0016-5085(96)70057-4. [DOI] [PubMed] [Google Scholar]

[bib88] Vanderwinden JM, Rumessen JJ, Bernex F, Schiffmann SN, Panthier JJ. Distribution and ultrastructure of interstitial cells of cajal in the mouse Colon, using antibodies to kit and kit(W-lacZ) mice. Cell and Tissue Research. 2000;302:155–170. doi: 10.1007/s004419900170. [DOI] [PubMed] [Google Scholar]

[bib89] Vierra NC, Kirmiz M, van der List D, Santana LF, Trimmer JS. Kv2.1 mediates spatial and functional coupling of L-type calcium channels and ryanodine receptors in mammalian neurons. eLife. 2019;8:e49953. doi: 10.7554/eLife.49953. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib90] Ward SM, Keller RG, Sanders KM. Structure and organization of electrical activity of canine distal Colon. American Journal of Physiology-Gastrointestinal and Liver Physiology. 1991;260:G724–G735. doi: 10.1152/ajpgi.1991.260.5.G724. [DOI] [PubMed] [Google Scholar]

[bib91] Ward SM, Burns AJ, Torihashi S, Sanders KM. Mutation of the proto-oncogene c-kit blocks development of interstitial cells and electrical rhythmicity in murine intestine. The Journal of Physiology. 1994;480:91–97. doi: 10.1113/jphysiol.1994.sp020343. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib92] Ward SM, Burns AJ, Torihashi S, Harney SC, Sanders KM. Impaired development of interstitial cells and intestinal electrical rhythmicity in steel mutants. American Journal of Physiology-Cell Physiology. 1995;269:C1577–C1585. doi: 10.1152/ajpcell.1995.269.6.C1577. [DOI] [PubMed] [Google Scholar]

[bib93] Ward SM, Beckett EA, Wang X, Baker F, Khoyi M, Sanders KM. Interstitial cells of cajal mediate cholinergic neurotransmission from enteric motor neurons. The Journal of Neuroscience. 2000a;20:1393–1403. doi: 10.1523/JNEUROSCI.20-04-01393.2000. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib94] Ward SM, Ordog T, Koh SD, Baker SA, Jun JY, Amberg G, Monaghan K, Sanders KM. Pacemaking in interstitial cells of cajal depends upon calcium handling by endoplasmic reticulum and mitochondria. The Journal of Physiology. 2000b;525:355–361. doi: 10.1111/j.1469-7793.2000.t01-1-00355.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib95] Won KJ, Sanders KM, Ward SM. Interstitial cells of cajal mediate mechanosensitive responses in the stomach. PNAS. 2005;102:14913–14918. doi: 10.1073/pnas.0503628102. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib96] Xu X, Best PM. Postnatal changes in T-type calcium current density in rat atrial myocytes. The Journal of Physiology. 1992;454:657–672. doi: 10.1113/jphysiol.1992.sp019285. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib97] Xu W, Lipscombe D. Neuronal ca(V)1.3alpha(1) L-type channels activate at relatively hyperpolarized membrane potentials and are incompletely inhibited by dihydropyridines. The Journal of Neuroscience. 2001;21:5944–5951. doi: 10.1523/JNEUROSCI.21-16-05944.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib98] Yoneda S, Takano H, Takaki M, Suzuki H. Properties of spontaneously active cells distributed in the submucosal layer of mouse proximal Colon. The Journal of Physiology. 2002;542:887–897. doi: 10.1113/jphysiol.2002.018705. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib99] Yoneda S, Takano H, Takaki M, Suzuki H. Effects of nifedipine and nickel on plateau potentials generated in submucosal interstitial cells distributed in the mouse proximal Colon. Journal of Smooth Muscle Research. 2003;39:55–65. doi: 10.1540/jsmr.39.55. [DOI] [PubMed] [Google Scholar]

[bib100] Yoneda S, Fukui H, Takaki M. Pacemaker activity from submucosal interstitial cells of cajal drives high-frequency and low-amplitude circular muscle contractions in the mouse proximal Colon. Neurogastroenterology and Motility. 2004;16:621–627. doi: 10.1111/j.1365-2982.2004.00546.x. [DOI] [PubMed] [Google Scholar]

[bib101] Youm JB, Zheng H, Koh SD, Sanders KM. Na-K-2Cl cotransporter and Store-Operated Ca2+ Entry in Pacemaking by Interstitial Cells of Cajal. Biophysical Journal. 2019;117:767–779. doi: 10.1016/j.bpj.2019.07.020. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib102] Zheng H, Park KS, Koh SD, Sanders KM. Expression and function of a T-type Ca2+ conductance in interstitial cells of cajal of the murine small intestine. American Journal of Physiology-Cell Physiology. 2014;306:C705–C713. doi: 10.1152/ajpcell.00390.2013. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib103] Zheng H, Drumm BT, Earley S, Sung TS, Koh SD, Sanders KM. SOCE mediated by STIM and orai is essential for pacemaker activity in the interstitial cells of cajal in the gastrointestinal tract. Science Signaling. 2018;11:eaaq0918. doi: 10.1126/scisignal.aaq0918. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib104] Zhu MH, Kim TW, Ro S, Yan W, Ward SM, Koh SD, Sanders KM. A ca(2+)-activated cl(-) conductance in interstitial cells of cajal linked to slow wave currents and pacemaker activity. The Journal of Physiology. 2009;587:4905–4918. doi: 10.1113/jphysiol.2009.176206. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib105] Zhu MH, Sung IK, Zheng H, Sung TS, Britton FC, O'Driscoll K, Koh SD, Sanders KM. Muscarinic activation of Ca2+-activated cl- current in interstitial cells of cajal. The Journal of Physiology. 2011;589:4565–4582. doi: 10.1113/jphysiol.2011.211094. [DOI] [PMC free article] [PubMed] [Google Scholar]

[bib106] Zhu MH, Sung TS, O'Driscoll K, Koh SD, Sanders KM. Intracellular ca(2+) release from endoplasmic reticulum regulates slow wave currents and pacemaker activity of interstitial cells of cajal. American Journal of Physiology-Cell Physiology. 2015;308:C608–C620. doi: 10.1152/ajpcell.00360.2014. [DOI] [PMC free article] [PubMed] [Google Scholar]

PERMALINK

Ca2+ signaling driving pacemaker activity in submucosal interstitial cells of Cajal in the murine colon

Salah A Baker

Wesley A Leigh

Guillermo Del Valle

Inigo F De Yturriaga

Sean M Ward

Caroline A Cobine

Bernard T Drumm

Kenton M Sanders

Roles

Abstract

Introduction

Results

ICC-SM distribution within the submucosal plexus

Figure 1. Distribution of Kit+ submucosal interstitial cells of Cajal (ICC-SM) in the murine colon.

Figure 2. Temporal sequence of Ca2+ transients firing in submucosal interstitial cells of Cajal (ICC-SM) and smooth muscle cells (SMCs).

Figure 3. Propagating Ca2+ waves in submucosal interstitial cells of Cajal (ICC-SM) network.

Pacemaker activity of ICC-SM drives responses in SMCs

Video 1. Simultaneous dual-color imaging of submucosal interstitial cells of Cajal (ICC-SM) and smooth muscle cells (SMCs) in the colon.

Global Ca2+-firing patterns in ICC-SM

Figure 4. Submucosal interstitial cells of Cajal (ICC-SM) Ca2+ signaling activity is entrained.

ICC-SM Ca2+ signals composed of multiple Ca2+-firing sites

Figure 5. Ca2+ transients in submucosal interstitial cells of Cajal (ICC-SM) arise from multiple firing sites.

Figure 6. Submucosal interstitial cells of Cajal (ICC-SM) Ca2+ transient initiation sites.

Video 2. High spatial resolution ICC-SM Ca2+ signals composed of multiple Ca2+-firing sites.

Ca2+ influx mechanisms are required for initiation of clustered Ca2+ transients in ICC-SM

Figure 7. The effect of lowering [Ca2+]o on Ca2+ transients in submucosal interstitial cells of Cajal (ICC-SM).

Molecular expression of Ca2+entry channels in ICC-SM

Figure 8. Molecular expression of genes related to Ca2+ entry channels.

L-type Ca2+ channels are important for organization of Ca2+ transients in ICC-SM

Figure 9. L-type Ca2+ channel antagonist, nicardipine effects on submucosal interstitial cells of Cajal (ICC-SM) Ca2+ transients.

Figure 9—figure supplement 1. Isradipine effects on Ca2+ transients in submucosal interstitial cells of Cajal (ICC-SM).

T-type Ca2+ channels contribute to Ca2+ transients in ICC-SM

Figure 10. T-type Ca2+ channel antagonist, NNC 55–0396 effects on ICC-SM Ca2+ transients.

Figure 10—figure supplement 1. Effects of T-type Ca2+ channel antagonists, TTA-A2 and Z-944 on submucosal interstitial cells of Cajal (ICC-SM) Ca2+ transients.

Effects of membrane hyperpolarization on Ca2+ transients in ICC-SM

Figure 11. The effects of membrane hyperpolarization and voltage-dependent Ca2+ entry block on submucosal interstitial cells of Cajal (ICC-SM) Ca2+ transients.

Figure 11—figure supplement 1. The effects of pinacidil of Ca2+ transients in submucosal interstitial cells of Cajal (ICC-SM).

Contributions of intracellular Ca2+ stores and release channels in ICC-SM Ca2+ activity

Figure 12. Intracellular Ca2+ stores contributions in submucosal interstitial cells of Cajal (ICC-SM) Ca2+ transients firing.

Figure 13. Ryanodine receptors (RyRs) contributing to Ca2+ release in submucosal interstitial cells of Cajal (ICC-SM).

Figure 13—figure supplement 1. IP3 receptors (IP3Rs) contribution to Ca2+ transients in submucosal interstitial cells of Cajal (ICC-SM).

Figure 13—figure supplement 2. Effects of 2-APB and Tetracaine on submucosal interstitial cells of Cajal (ICC-SM) Ca2+ transients.

Figure 14. SOCE role in maintaining submucosal interstitial cells of Cajal (ICC-SM) Ca2+ transients.

Discussion

Figure 15. Role of voltage-dependent Ca2+ entry in the pacemaker function of submucosal interstitial cells of Cajal (ICC-SM).

Figure 15—figure supplement 1. Schematic showing how Ca2+ signaling in the pacemaker function of submucosal interstitial cells of Cajal (ICC) is coupled to smooth muscle cells (SMCs).

Materials and methods

Key resources table.

Animals

Generation of Kit-iCre-GCaMP6f/Acta2-RCaMP1.07 mice

Tissue preparation

Immunohistochemistry

Cell sorting and quantitative PCR

Ca2+ imaging

Ca2+ imaging analysis

Drugs and solutions

Statistical analysis

Acknowledgements

Funding Statement

Contributor Information

Funding Information

Additional information

Competing interests

Author contributions

Ethics

Additional files

Data availability

References

Decision letter

Roles

Author response

Associated Data

Supplementary Materials

Data Availability Statement

ACTIONS

PERMALINK

RESOURCES

Similar articles

Ca²⁺ signaling driving pacemaker activity in submucosal interstitial cells of Cajal in the murine colon

Figure 1. Distribution of Kit⁺ submucosal interstitial cells of Cajal (ICC-SM) in the murine colon.

Figure 2. Temporal sequence of Ca²⁺ transients firing in submucosal interstitial cells of Cajal (ICC-SM) and smooth muscle cells (SMCs).

Figure 3. Propagating Ca²⁺ waves in submucosal interstitial cells of Cajal (ICC-SM) network.

Global Ca²⁺-firing patterns in ICC-SM

Figure 4. Submucosal interstitial cells of Cajal (ICC-SM) Ca²⁺ signaling activity is entrained.

ICC-SM Ca²⁺ signals composed of multiple Ca²⁺-firing sites

Figure 5. Ca²⁺ transients in submucosal interstitial cells of Cajal (ICC-SM) arise from multiple firing sites.

Figure 6. Submucosal interstitial cells of Cajal (ICC-SM) Ca²⁺ transient initiation sites.

Video 2. High spatial resolution ICC-SM Ca²⁺ signals composed of multiple Ca^2+-firing sites.

Ca²⁺ influx mechanisms are required for initiation of clustered Ca²⁺ transients in ICC-SM

Figure 7. The effect of lowering [Ca²⁺]_o on Ca²⁺ transients in submucosal interstitial cells of Cajal (ICC-SM).

Molecular expression of Ca²⁺entry channels in ICC-SM

Figure 8. Molecular expression of genes related to Ca²⁺ entry channels.

L-type Ca²⁺ channels are important for organization of Ca²⁺ transients in ICC-SM

Figure 9. L-type Ca²⁺ channel antagonist, nicardipine effects on submucosal interstitial cells of Cajal (ICC-SM) Ca²⁺ transients.

Figure 9—figure supplement 1. Isradipine effects on Ca²⁺ transients in submucosal interstitial cells of Cajal (ICC-SM).

T-type Ca²⁺ channels contribute to Ca²⁺ transients in ICC-SM

Figure 10. T-type Ca²⁺ channel antagonist, NNC 55–0396 effects on ICC-SM Ca²⁺ transients.

Figure 10—figure supplement 1. Effects of T-type Ca²⁺ channel antagonists, TTA-A2 and Z-944 on submucosal interstitial cells of Cajal (ICC-SM) Ca²⁺ transients.

Effects of membrane hyperpolarization on Ca²⁺ transients in ICC-SM

Figure 11. The effects of membrane hyperpolarization and voltage-dependent Ca²⁺ entry block on submucosal interstitial cells of Cajal (ICC-SM) Ca²⁺ transients.

Figure 11—figure supplement 1. The effects of pinacidil of Ca²⁺ transients in submucosal interstitial cells of Cajal (ICC-SM).

Contributions of intracellular Ca²⁺ stores and release channels in ICC-SM Ca²⁺activity

Figure 12. Intracellular Ca²⁺ stores contributions in submucosal interstitial cells of Cajal (ICC-SM) Ca²⁺ transients firing.

Figure 13. Ryanodine receptors (RyRs) contributing to Ca²⁺ release in submucosal interstitial cells of Cajal (ICC-SM).

Figure 13—figure supplement 1. IP₃ receptors (IP₃Rs) contribution to Ca²⁺ transients in submucosal interstitial cells of Cajal (ICC-SM).

Figure 13—figure supplement 2. Effects of 2-APB and Tetracaine on submucosal interstitial cells of Cajal (ICC-SM) Ca²⁺ transients.

Figure 14. SOCE role in maintaining submucosal interstitial cells of Cajal (ICC-SM) Ca²⁺ transients.

Figure 15. Role of voltage-dependent Ca²⁺ entry in the pacemaker function of submucosal interstitial cells of Cajal (ICC-SM).

Figure 15—figure supplement 1. Schematic showing how Ca²⁺ signaling in the pacemaker function of submucosal interstitial cells of Cajal (ICC) is coupled to smooth muscle cells (SMCs).

Ca²⁺ imaging

Ca²⁺ imaging analysis